Changes in ovarian structures, blood biochemical and hormonal profile in double Ovsynch treated conceived and non-conceived Jaffarabadi buffaloes

R J RAVAL; T K PATBANDHA; B J TRANGADIA; A J DHAMI; F S KAVANI

doi:10.56093/ijans.v92i8.115682

Authors

R J RAVAL Kamdhenu University, Gujarat
T K PATBANDHA
B J TRANGADIA Kamdhenu University, Gujarat
A J DHAMI Anand Agricultural University, Anand-388001, Gujarat, India
F S KAVANI Anand Agricultural University, Anand-388001, Gujarat, India

https://doi.org/10.56093/ijans.v92i8.115682

Keywords:

Double Ovsynch, Hormonal profile, Jaffarabadi buffalo, Ovarian changes

Abstract

The present study comprised 12 post-pubertal acyclic buffalo heifers (40-50 months of age) and 6 postpartum lactating acyclic buffalo cows. Animals were monitored for ovarian changes by ultrasonography along with blood sampling on different days of hormonal treatment, on day 27 of protocol and on day 12, 21 and 35 post-insemination. Total 10 animals (55.56%; 9/12 heifers; 1/6 buffalo) conceived at fix timed artificial insemination (FTAI) and remaining 8 animals were considered as non-conceived for comparison. Significantly higher number of large follicles was observed on day 7 with reduction in mean diameter of subordinate follicles at day 26, decreased plasma levels of FSH on day 17, 24 and 26; increased plasma level of LH on day 27, with higher insulin level in conceived animals as compared to non-conceived animals. Significant reduction in number of large follicles was recorded on day 21 post- AI in conceived animals. The difference in the levels of progesterone was statistically significant between conceived and non-conceived animals at day 35 post-AI. There were highly significant positive correlations among population of different size follicles, total follicles and largest and subordinate follicles diameter, whereas they had negative correlation with plasma protein and cholesterol both in conceived and non-conceived animals, but their correlations with plasma LH, insulin and estrogen were significant and positive only among non-conceived group.

Downloads

Download data is not yet available.

References

Anitha, A, Rao S K, Suresh J, Moorthy P R S and Reddy Y K. 2011. A body condition score (BCS) system in Murrah buffaloes. Buffalo Bulletin 30(1): 79–99.

Anonymous. 2016. AGRESCO report of Cattle Breeding Farm, JAU, Junagadh. Submitted for Twelfth Combined Joint Agricultural Research Council meeting of SAUs Gujarat held at NAU, Navsari, Gujarat, India, pp. 6–8.

Barile V L, Terzano G M, Allegrini S, Maschio M, Razzano M, Neglia G and Pacelli C. 2007. Relationship among preovulatory follicle, corpus luteum and progesterone in oestrus synchronized buffaloes. Italian Journal of Animal Sciences 6: 663–66. DOI: https://doi.org/10.4081/ijas.2007.s2.663

Butler W R. 1999. Effects of energy balance on folicular development and first ovulation in postpartum dairy cows. Journal of Reproduction and Fertility 54: 411–24.

Campanile G, Di Palo R, Neglia G, Vecchio D, Gasparrini B, Prandi A, Galiero G and D’occhio M J. 2007. Corpus luteum function and embryonic mortality in buffaloes treated with a GnRH agonist, hCG and progesterone. Theriogenology 67: 1393–98. DOI: https://doi.org/10.1016/j.theriogenology.2007.03.001

Chaudhary N J, Patel D M, Dhami A J, Vala K B, Hadiya K K and Patel J A. 2018. Effect of Doublesynch and Estradoublesynch protocols on estrus induction, conception rate, plasma progesterone, protein, and cholesterol profile in anestrus Gir heifers. Veterinary World 11: 542-48. DOI: https://doi.org/10.14202/vetworld.2018.542-548

Cirit U, Ak K and Ileri I K. 2007. New strategies to improve the efficiency of the Ovsynch protocol in primiparous dairy cows. Bulletin of the Veterinary Institute in Pulawy 51: 47–51.

De Rensis F, Ronci G, Guarneri P, Nguyen B X, Presicce G A, Huszenicza G and Scaramuzzi R J. 2005. Conception rate after fixed time insemination following ovsynch protocol with and without progesterone supplementation in cyclic and non-cyclic Mediterranean Italian buffaloes (Bubalus bubalis). Theriogenology 63: 1824–31. DOI: https://doi.org/10.1016/j.theriogenology.2004.07.024

Dhindsa S S, Honparkhe M, Grewal R S and Brar P S. 2016. Fertility enhancement through Doublesynch protocol in buffalo during summer season. Indian Veterinary Journal 93: 17–19.

Dirandeh E, Roodbari A R and Colazo M G. 2015. Double-ovsynch, compared with presynch with or without GnRH, improves fertility in heat-stressed lactating dairy cows. Theriogenology 83: 438–43. DOI: https://doi.org/10.1016/j.theriogenology.2014.10.011

ICAR. 2013. Nutrient Requirements of Animal-Cattle and Buffalo. 3rd Edn. Indian Council of Agricultural Research, New Delhi, India.

Kaneko H, Krishi H, Watanabe G, Taya K, Sasamoto S and Hasegawa Y. 1995. Changes in plasma concentrations of immunoreactive inhibin, estradiol and FSH associated with follicular waves during the estrous cycle of the cow. Journal of Reproduction and Development 41: 311–20. DOI: https://doi.org/10.1262/jrd.41.311

Kavani F S, Khasatiya C T, Sthanki D J, Thakor D B, Dhami A J and Panchal M T. 2005. Studies on postpartum biochemical and hormonal profile of fertile and infertile oestrus cycles in Surti buffaloes. Indian Journal of Animal Reproduction 26: 1–6.

Kumar Parveen. 2015. ‘Comparative study on Ovsynch versus Doublesynch protocol for estrus synchronization during summer and winter season in buffalo.’ Ph.D. Thesis, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana, India.

Lopes A S, Butler S T, Gilbert R O and Butler W R. 2007. Relationship of preovulatory follicle size, estradiol concentrations and season to pregnancy outcome in dairy cows. Animal Reproduction Science 99: 34–43. DOI: https://doi.org/10.1016/j.anireprosci.2006.04.056

Lynch C O, Kenny D A, Childs S and Diskin M G. 2010. The relationship between periovulatory endocrine and follicular activity on corpus luteum size, function, and subsequent embryo survival. Theriogenology 73(2): 190–98. DOI: https://doi.org/10.1016/j.theriogenology.2009.08.012

Malik R K. 2005. ‘Studies on ovarian follicular dynamics during early postpartum period, anestrus condition and hormonal therapies for induction of estrus in Murrah buffaloes (Bubalus bubalis).’ Ph.D. Thesis, CCS Haryana Agricultural University, Hisar, India.

Martins J P, Policelli R K, Neuder L M, Raphael W and Pursley J R. 2011. Effects of cloprostenol sodium at final prostaglandin F2α of Ovsynch on complete luteolysis and pregnancy per artificial insemination in lactating dairy cows. Journal of Dairy Science 94: 2815–24. DOI: https://doi.org/10.3168/jds.2010-3652

Mohan K, Sarkar M and Prakash B S. 2009. Efficiency of Heatsynch protocol in estrous synchronization, ovulation and conception of dairy buffaloes (Bubalus bubalis). Asian Australasian Journal of Animal Sciences 22: 774–80. DOI: https://doi.org/10.5713/ajas.2009.80524

Nebel R L and Mcgilliard M L. 1993. Interactions of high milk yield and reproductive performance in dairy cows. Journal of Dairy Science 76: 3257–68. DOI: https://doi.org/10.3168/jds.S0022-0302(93)77662-6

Patel K R, Dhami A J, Hadiya K K, Savalia K K and Sarvaiya N P. 2013. Effect of CIDR and Ovsynch protocols on estrus response, fertility and plasma progesterone and biochemical profile in true anoestrus crossbred cows. Indian Journal of Animal Production and Management 29: 50–58.

Perry G A, Smith M F, Roberts A J, Mac Neil M D and Geary T W. 2007. Relationship between size of the ovulatory follicle and pregnancy success in beef heifers. Journal of Animal Science 85: 684–89. DOI: https://doi.org/10.2527/jas.2006-519

Prajapati J P, Patel D M, Dhami A J, Patel J A, Hadiya K K and Chaudhary D V. 2018. Role of various estrus induction and synchronization protocols in influencing plasma progesterone, metabolic profile and fertility in acyclic buffaloes. Indian Journal of Veterinary Science and Biotechnology 14: 1–7. DOI: https://doi.org/10.21887/ijvsbt.v14i1.12988

Pursley J R, Mee M O and Wiltbank M C. 1995. Synchronization of ovulation in dairy cows using PGF2alpha and GnRH. Theriogenology 44: 915–23. DOI: https://doi.org/10.1016/0093-691X(95)00279-H

Silva E, Sterry R A and Fricke P M. 2007. Assessment of a practical method for identifying anovular dairy cows synchronized for first postpartum timed artificial insemination. Journal of Dairy Science 90: 3255–62. DOI: https://doi.org/10.3168/jds.2006-779

Snedecor G W and Cochran W G. 1994. Statistical Methods. 8th Edn. Oxford and IBH Publishing Company, Calcutta, India.

Souza A H, Ayres H, Ferreira R M and Wiltbank M C. 2008. A new presynchronization system (Double-ovsynch) increases fertility at first postpartum timed AI in lactating dairy cows. Theriogenology 70(2): 208–15. DOI: https://doi.org/10.1016/j.theriogenology.2008.03.014

Stevenson J. 2017. Ovsynch goes double. Hoard’s dairy man. Available at http://hourds. com/print-article-4777-permanent. html> accessed on 17 July, 2017

Vasconcelos J L M, Silcox R W, Rosa G J M, Pursely J R and Wiltbank M C. 1999. Synchronization rate, size of the ovulatory follicle and pregnancy rate after synchronization of ovulation beginning on different days of the estrous cycle in lactating dairy cows. Theriogenology 52: 1067–78. DOI: https://doi.org/10.1016/S0093-691X(99)00195-8

Wiltbank M C, Arvalho C P D, Keskin A et al. 2011. Effect of progesterone concentration during follicle development on subsequent ovulation, fertilization, and early embryo development in lactating dairy cows. Biology of Reproduction 85: 685 (Abstract). DOI: https://doi.org/10.1093/biolreprod/85.s1.685