Association of neuropeptide-Y gene polymorphic variants with quantitative traits in Jabalpur colour and Kadaknath chicken

PRATIBHA PADWAR; M S THAKUR

doi:10.56093/ijans.v91i9.116461

Authors

PRATIBHA PADWAR Nanaji Deshmukh Veterinary Science University, Jabalpur, Madhya Pradesh 482 001 India
M S THAKUR Nanaji Deshmukh Veterinary Science University, Jabalpur, Madhya Pradesh 482 001 India

https://doi.org/10.56093/ijans.v91i9.116461

Keywords:

Chicken, Jabalpul colour, Kadaknath, NPY gene, PCR-RFLP, Polymorphism, Quantitative traits

Abstract

The present study included 60 birds of Kadaknath and Jabalpur colour to explore polymorphism of NPY gene and its association with quantitative traits. PCR-RFLP assay of NPY/Dra1 gene (240 bp) locus revealed three genotypes, viz. AA (240 bp/240 bp), AB (240/161/79 bp) and BB (161/79 bp) in Kadaknath and Jabalpur colour chicken. Genotype AA (240 bp/240 bp) was predominately found in both the chicken population. The population of Kadaknath was in Hardy Weinberg equilibrium, while Jabalpur colour was not in HWE at NPY gene locus. Analysis of variance showed highly significant differences between breeds, whereas the effect of genotypes and breed Ã— genotype interaction was non-significant. The means of body weight at sexual maturity, body weight at 20, 30 and 40 weeks, egg weight and egg production at 40 week and shape index were significantly higher in Jabalpur colour than Kadaknath. Although the effect of genotypes was non-significant but comparatively higher adult body weight was recorded for AA genotype in Jabalpur colour and AB genotype in Kadaknath at 20, 30 and 40 weeks of age. Similarly, AA genotype was marginally superior in Jabalpur colour, while genotype BB exhibited marginal superiority in Kadaknath for egg production at 40 weeks of age.

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References

Alameri M S S, Al-Anbari E H H and Erzuky W M. 2019. Association the Neuropeptides-Y (NPY) gene polymorphisms

with egg production traits in Iraqi local Brown chicken. Biochemical and Cellular Archives 19: 1381–88.

Chau T V and Nguyen T N. 2016. Single nucleotide polymorphism in candidate genes associated with egg production traits native NOI chicken of Vietnam. International Journals of Plants, Animals and Environmental Science 6: 162–69.

Harvey W R. 1990. User’s guide for LSMLMW and MIXMOL PC-2 Version. Ohio, U.S.A.

John S W, Weitzner G, Rozen R and Scriver C R. 1991. A rapid procedure for extracting genomic DNA from leukocytes. DOI: https://doi.org/10.1093/nar/19.2.408

Nucleic Acids Research 19: 408.

Kumar N, Belay Z N, Asfaw Y T and Kebede E. 2014. Evaluation of egg quality traits of Rhode Island Red and Bovans White under intensive management in Mekelle, Ethiopia. Journal of Agriculture and Veterinary Science 7: 71–75. DOI: https://doi.org/10.9790/2380-07227175

Li H F, Zhu W Q, Chen K W, Wu X, Tang Q P, Gao Y S, Song W J and Xu H L. 2009. Polymorphism in NPY and IGF-1 genes associate with reproductive traits in Wenchang chicken. African Journal of Biotechnology 8: 4744–48.

Nguyen T N, Nguyen H X, Chau T V, Nguyen T A, Tran N D and Nguyen T H N. 2015. Effect of polymorphism on egg

production in indigenous NOI chicken. Journal of Experimental Biology and Agricultural Sciences 3: 487–93.

Parmar S N S, Thakur M S, Tomar S S and Pillai P V A. 2006. Evaluation of egg quality traits in indigenous Kadaknath breed of poultry. Livestock Research and Rural Development 18: 1–4.

Rath P K, Mishra P K, Mallick B K and Behura N C. 2015. Evaluation of different egg quality traits and interpretation of

their mode of inheritance in White Leghorns. Veterinary World 8: 449–52.

Sambrook J and Russell D. 2001. Molecular Cloning: A Laboratory Manual. 3rd edn. Cold Spring Harbor Laboratory

Press, Cold Spring Harbor, NY.

Singh R A. 1985. Poultry Production. 2nd edn, Kalyani Publishers, Daryaganj, New Delhi (India).

Thakur M S, Parmar S N S, Chaudhari M V and Bhardwaj J K. 2009. Growth hormone gene polymorphism and its association with egg production in Kadaknath chicken. Livestock Research for Rural Development 21: 1–9.

Xu H, Zeng H, Luo C, Zhang D, Wang Q, Sun L, Yang L, Zhou M, Nie Q and Zhang X. 2011. Genetic effects of

polymorphisms in candidate genes and the QTL region on chicken age at first egg. BMC Genetics 12: 1–9.

Yeh F C, Yang R C and Boyle T. 1999. POPGENE 32 – Version 1.31. Population genetics software. http.//www.ualberta.ca/~fyeh/fyeh.