Effect of Superoxide dismutase on cryopreservation of mithun semen
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https://doi.org/10.56093/ijans.v92i6.116624
Keywords:
Cryopreservation, Kinetic profiles, Mithun, Semen, Superoxide dismutaseAbstract
The present study was designed to assess the effect of superoxide dismutase (SOD) on post thaw semen quality parameters (SQPs), kinetic profiles and seminal biochemical profiles in mithun. A total of 25 ejaculates were selected and each was split into four equal aliquots after dilution with Tris-citrate-glycerol (TCG) extender such as Group I: control, Group II, III and IV contained 50, 100 and 150 U/ml of SOD, respectively. Cryopreserved and thawed samples were analysed for their motility parameters (progressive forward and in bovine cervical mucus penetration test [BCMPT]), kinetic and velocity parameters by computer assisted sperm analyser (CASA), viability, sperm morphological and nuclear abnormalities, acrosomal, plasma membrane and nuclear integrities and sperm enzymatic leakage and biochemical (sperm cholesterol and antioxidant and oxidative stress) profiles. Study revealed a significant (p <0.05) enhancement in viability, sperm morphological and nuclear normalities, acrosome integrity, motility (progressive and BCMPT), sperm cholesterol content and reduction in leakage of intracellular enzymes in Group III. Moreover, intactness of acrosome and biochemical membranes were protected significantly (p < 0.05) in addition to significant (p < 0.05) improvement in kinetic and velocity profiles in extender containing 100 U/mL SOD. These results clearly suggest that however the cryopreservation of mithun’s spermatozoa in TCG was comparable with other species and inclusion of 100 U/mL SOD holds a clear advantage over control or 50 or 150 U/mL SOD. It can be concluded that SOD supplementation in semen extender can be effectively utilized to reduce the oxidative stress and improved the antioxidant profiles with cascading beneficial effects on cryopreserved semen quality parameters in mithun.Downloads
References
Aitken J and Fisher H. 1994. Reactive oxygen species generation and human spermatozoa: the balance of benefit and risk. Bioassays 16(4): 259–67.
Aitken R J, De Luliis G N, Finnie J M, Hedges A and McLachlan R. 2010. Analysis of the relationships between oxidative stress, DNA damage and sperm vitality in a patient population: Development of diagnostic criteria. Human Reproduction
(10): 2415–26.
Alvarez J G and Storey B T. 1992. Evidence for increased lipid peroxidative damage and loss of superoxide dismutase activity
as a model of sub lethal cryo damage to human sperm during cryopreservation. Journal of Andrology 13(3): 232–41.
Asadpour R, Jafari R and Tayefi-Nasrabadi H. 2012. The effect of antioxidant supplementation in semen extenders on semen
quality and lipid peroxidation of chilled bull spermatozoa. Iranian Journal of Veterinary Research 13(3): 246–49.
Barth A D and Oko R J. 1989. Preparation of semen for morphological examination. Abnormal Morphology of Bovine
Spermatozoa. Iowa State University Press, Ames, IA. p. 8–18. Bernardini A, Hozbor F, Sanchez E, Fornés M, Alberio R and Cesari A. 2011. Conserved ram seminal plasma proteins bind to the sperm membrane and repair cryopreservation damage. Theriogenology 76: 436–47.
Bilodeau J F, Blanchette S, Gagnon C and Sirard M A. 2001. Thiols prevent H2O2-mediated loss of sperm motility in cryopreserved bull semen. Theriogenology 56(2): 275–86. Bligh E G and Dyer W J. 1959. A rapid method of total lipid extraction and purification. Canadian Journal of Biochemistry and Physiology 37: 911–17.
Brooks D E. 1990. Biochemistry of the male accessory glands. Marshall’s Physiology of Reproduction.(Ed.) Lamming G E. 4th edn. Churchill Livingstone, Edinburgh. pp. 569–690. Buckland R B. 1971. The activity of six enzymes of chicken seminal plasma and sperm. 1. Effect of in vitro storage and full sib families on enzyme activity and fertility. Poultry Science 50(6): 1724–34.
Chatterjee S, de Lamirande E and Gagnon C. 2001. Cryopreservation alters membrane sulfhydryl status of bull spermatozoa: Protection by oxidized glutathione. Molecular Reproduction and Development 60: 498–506. Cocchia N, Pasolini M P, Mancini R, Petrazzuolo O, Cristofaro I, Rosapane I, Sica A, Tortora G, Lorizio R, Paraggio G and Mancini A. 2011. Effect of SOD (superoxide dismutase)
protein supplementation in semen extenders on motility, viability, acrosome status and ERK (extracellular signalregulated kinase) protein phosphorylation of chilled stallion spermatozoa. Theriogenology 75: 1201–10.
Corteel J M. 1980. Effects du plasma séminal sur la survie et la fertilité des spermatozoids conservés in vitro. Reproduction Nutrition Development 20(4): 1111-23. de Lamirande E and Gagnon C. 1995. Impact of reactive oxygen species on spermatozoa: A balancing act between beneficial and detrimental effects. Human Reproduction 10(1): 15–21.
El-Sisy G A, El- Nattat W S and El- Sheshtawy R I. 2008. Effect of superoxide dismutase and catalase on viability of cryopreserved buffalo spermatozoa. Global Veterinaria 2(2): 61–65. Gavella M, Lipovac V, Vucic M and Rocic B. 1996. Relationship of sperm superoxide ismutase-like activity with other sperm specific enzymes and experimentally induced lipid peroxidation in infertile men. Andrologia 28(4): 223–29.
Griveau J F, Dumont E, Renard P, Callegari J P and Le Lannou D. 1995. Reactive oxygen species, lipid peroxidation and enzymatic defence systems in human spermatozoa. Journal of Reproduction and Fertility 103(1): 17–26. Gundogan M. 2006. Some reproductive parameters and seminal plasma constituents in relation to season in Akkaraman and Awassi Rams. Turkish Journal of Veterinary and Animal Science 30(1): 95–100.
Holt W V. 2000. Fundamental aspects of sperm cryobiology: The importance of species and individual differences. Theriogenology 53(1): 47–58.
Jayaganthan P, Perumal P, Balamurugan T C, Verma R P, Singh L P, Pattanaik A K and Meena K. 2013. Effects of Tinospora cordifolia supplementation on semen quality and hormonal profile of ram. Animal Reproduction Science 140(1): 47–53.
Jeyendran R S, Vander Ven H H, Parez-Pelaez M, Crabo B G and Zaneweld L J D. 1984. Development of an assay to assess the functional integrity of the human membrane and its relationship to other semen characteristics. Journal of Reproduction and Fertility 70: 219–28.
Kankofer M, Kolm G, Aurich J and Aurich C. 2005. Activity of glutathione peroxidase, super oxide dismutase and catalaseand lipid peroxidation intensity in stallion semen during storage at 5°C. Theriogenology 63(5): 1354–65.
Kobayashi M, Kakizono T and Nagai S. 1991. Astaxanthin production by a green alga, Haematococcus pluvialis accompanied with morphological changes in acetate media. Journal of Fermentation and Bioengineering 71(5): 335–39.
Kumar R, Jagan Mohanarao G, Arvind R and Atreja S K. 2011. Freeze-thaw induced genotoxicity in buffalo (Bubalus bubalis)
spermatozoa in relation to total antioxidant status. Molecular Biology Report 38(3): 1499–1506. Lasley J F and Bogart R. 1944. A comparative study of epididymal and ejaculated spermatozoa of boar. Journal of Animal Science 3: 360–70.
Maxwell W M C and Stojanov T. 1996. Liquid storage of ram semen in the absence or presence of some antioxidants. Reproduction, Fertility and Development 8(6): 1013–20.
Moore A I, Squires E L and Graham J K. 2005. Adding cholesterol to the stallion sperm plasma membrane improves cryosurvival.
Cryobiology 51(3): 241–49.
Perumal P, Chang S, Baruah K K and Srivastava N. 2018. Administration of slow release exogenous melatonin modulates, oxidative stress profiles and in vitro fertilizing ability of the cryopreserved Mithun spermatozoa. Theriogenology 120: 79–90.
Perumal P, Chang S, Khate K, Vupru K and Bag S. 2019. Feeding supplementation of Flaxseed oil modulates semen production
and its quality parameters, freezability, oxidative stress profiles, scrotal and testicular biometrics and endocrinological
profiles in Mithun. Theriogenology 136: 47–59.
Perumal P, Selvaraju S, Selvakumar S, Barik A K, Mohanty D N, Das R K, Das S and Mishra P C. 2011. Effect of pre-freeze addition of cysteine hydrochloride and reduced glutathione in semen of crossbred Jersey bulls on sperm parameters and conception rates. Reproduction in Domestic Animal 46(4): 636–41.
Perumal P, Srivastava S K, Ghosh S K and Baruah K K. 2014. Computer-assisted sperm analysis of freezable and nonfreezable
Mithun (Bos frontalis) semen. Journal of Animals 2014: 1–6.
Perumal P, Vupru K and Rajkhowa C. 2013. Effect of addition of reduced glutathione on the liquid storage (5°C) of Mithun
(Bos frontalis) semen. Indian Journal of Animal Sciences 83(10): 1024–28.
Perumal P. 2014. Effect of superoxide dismutase on the liquid storage (5oC) of Mithun (Bos frontalis) semen. Journal of
Animals 2014: 1–9. Prasad J K, Kumar S, Mohan G, Agarwal S K and Shankar U.
Simple modified method for cervical mucus penetration test for quality assessment of bull semen. Indian Journal of
Animal Sciences 69: 103–05.
Reitman S and Frankel S A. 1957. Colorimetric method for the determination of serum oxaloacetic and glutamic pyruvate
transaminase. American Journal of Clinical Pathology 28: 56–63.
Salisbury G W, VanDemark N L and Lodge J R. 1985. Physiology of Reproduction and Artificial Insemination of Cattle. 2nd ed.
W.H. Freeman and Company. p. 268–274. Shoae A and Zamiri M J. 2008. Effect of butylated hydroxytoluene
on bull sperm frozen in egg yolk-citrate extender. Animal Reproduction Science 104(2): 414–18.
Sinha M P, Sinha A K, Sinka B K and Prasad P I. 1996. The effect of glutathione on motility, enzyme leakage and fertility of
frozen goat semen. Theriogenology 41: 237–43.
Suleiman S A, Ali M E, Zaki M S, Malik E M E A and Nast M A. 1996. Lipid peroxidation and human sperm motility:
Protective role of vitamin E. Journal of Andrology 17(5): 530–37.
Watson P F. 1975. Use of Giemsa stain to detect change in acrosome of frozen ram spermatozoa. Veterinary Record 97: 12–15.
Wotten I D P. 1964. Micro-analysis in Medical Biochemistry. 4th edn. J and A Churchill Ltd, London.
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