Genetic polymorphism of four candidate genes in dairy cattle of Kashmir, India


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Authors

  • RUKSANA SHAH Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Jammu and Kashmir 190 006 India
  • N A GANAI Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Jammu and Kashmir 190 006 India
  • S SHANAZ Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Jammu and Kashmir 190 006 India
  • F D SHEIKH Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Jammu and Kashmir 190 006 India
  • H M KHAN Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Jammu and Kashmir 190 006 India
  • NUSRAT N KHAN Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Jammu and Kashmir 190 006 India
  • AMBREEN HAMADANI Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Jammu and Kashmir 190 006 India
  • SAFEER ALAM Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Jammu and Kashmir 190 006 India

https://doi.org/10.56093/ijans.v91i10.117218

Keywords:

Candidate genes, Dairy cattle, Kashmir, Polymorphism

Abstract

Genetic information is necessary to devise strategic plans aimed to improve the genetic merit of dairy cattle. Exploration of quantitative, qualitative, and molecular genetics is important to improve dairy cattle performance. The aim of the study was to detect the presence of and to identify the polymorphism of kappa-casein (κ-CN), betalactoglobulin (β-LG), prolactin (PRL) and pituitary inhibition factor 1 (PIT-1) in 120 dairy cows of two genetic groups; Jersey and crossbred HF cows (60 each) maintained at an organized farm in Kashmir. PCR-RFLP of all selected candidate genes revealed ‘KK’, ‘Kk’ and ‘kk’ for HF crossbred cows and ‘KK’ and ‘Kk’ genotypes in Jersey cows for κ-CN gene. All animals under study showed 2, viz. Bb and bb genotypes for β-LG gene. In crossbred cows, all 3 patterns ‘RR’, ‘Rr’ and ‘rr’ were present but in Jersey cows, the genotype ‘rr’ was absent for PRL gene. In the genetic groups under study, 2 genotypes ‘Pp’ and ‘pp’ were found for PIT-1 gene. Genotypes Kk, Bb, Rr and pp were most prevalent in our selected population for κ-CN, β-LG, PRL and PIT-1 genes, respectively. Frequencies of K, b, R and p were found to be high for κ-CN, β-LG, PRL and PIT-1 genes, respectively. Chi-square analysis and p-values showed that the population does not vary significantly and proves that the population is in equilibrium. Our populations of Jersey and crossbred Friesian Holstein cattle were polymorphic. Genotypes Kk, Bb, Rr and pp were most prevalent in our population for κ-CN, β-LG, PRL and PIT-1 genes, respectively. The Chi-square and p-values showed that the population does not vary significantly and proves that the population is in equilibrium.

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Submitted

2021-10-26

Published

2021-10-26

Issue

Vol. 91 No. 10 (2021)

Section

Short-Communication

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The copyright of the articles published in The Indian Journal of Animal Sciences is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.

How to Cite

SHAH, R., GANAI, N. A., SHANAZ, S., SHEIKH, F. D., KHAN, H. M., KHAN, N. N., HAMADANI, A., & ALAM, S. (2021). Genetic polymorphism of four candidate genes in dairy cattle of Kashmir, India. The Indian Journal of Animal Sciences, 91(10), 851–855. https://doi.org/10.56093/ijans.v91i10.117218
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