Genetic diversity of Pasteurella multocida isolates of Indian origin

MADHU MISHRA; PRASAD THOMAS; SANTOSH KUMAR; SANTOSH K GUPTA; RAJESH K AGARWAL; VISWAS K NAGALEEKAR

doi:10.56093/ijans.v91i11.118129

Authors

MADHU MISHRA Government of Madhya Pradesh, Teekamgarh, Madhya Pradesh
PRASAD THOMAS ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India
SANTOSH KUMAR ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India
SANTOSH K GUPTA Krishi Vigyan Kendra, Saraiya, Muzaffarpur, Bihar
RAJESH K AGARWAL ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India
VISWAS K NAGALEEKAR ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India

https://doi.org/10.56093/ijans.v91i11.118129

Keywords:

Epidemiological typing, India, MLST, Pasteurella multocida

Abstract

In the current study, correlation among specific capsular types, sequence types and specific hosts were observed. Study proves high diversity with respect to small ruminant isolates belonging to capsular type A. Higher sharing of sequence types among small ruminant, porcine and avian hosts indicate the close association of these isolates getting adapted for diverse host species. The study also shows that CC229, mostly from small ruminant hosts, was genetically related to HS-specific ST122 indicating recent diversification and possibly, similar genotype and pathogenesis.

Downloads

Download data is not yet available.

References

Aiswarya V, Yogesh A C, Bharat B B, Rafiyuddin A, Mathakiya and Roy A. 2017. Multilocus sequence typing of P. multocida isolates of buffalo origin from Gujarat state of India. Buffalo Bulletin 36(2): 385–99.

Einarsdottir T, Gunnarsson E, Sigurdardottir O G, Jorundsson E, Fridriksdottir V, Thorarinsdottir G E and Hjartardottir S. 2016. Variability of Pasteurella multocida isolated from Icelandic sheep and detection of the toxA gene. Journal of Medical Microbiology 65(9): 897–904.

Hall T A. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41: 95–98.

Hotchkiss E J, Hodgson J C, Lainson F A and Zadoks R N. 2011. Multilocus sequence typing of a global collection of

Pasteurella multocida isolates from cattle and other host species demonstrates niche association. BMC Microbiology

: 115–15.

Jolley K A, Chan M S and Maiden M C. 2004. mlstdbNetdistributed multi-locus sequence typing (MLST) databases.

BMC Bioinformatics 5: 86.

Letunic I and Bork P. 2016. Interactive tree of life (iTOL) v3: An online tool for the display and annotation of phylogenetic and other trees. Nucleic Acids Research 44(W1): W242–45.

Maiden M C J, Bygraves J A, Feil E, Morelli G, Russell J E, Urwin R, Zhang Q, Zhou J, Zurth K, Caugant D A, Feavers I

M, Achtman M and Spratt B G. 1998. Multilocus sequence typing: A portable approach to the identification of clones within populations of pathogenic microorganisms. Proceedings of the National Academy of Sciences 95(6): 3140–45.

Mir R, Thomas P, Viswas K, Gupta S, Verma J, Singh A and Singh V. 2011. Multilocus sequence typing of Indian isolates

of Pasteurella multocida. Indian Journal of Comparative Microbiology, Immunology and Infectious Diseases 32: 30–35.

Petersen A, Bisgaard M, Townsend K and Christensen H. 2014. MLST typing of Pasteurella multocida associated with

haemorrhagic septicaemia and development of a real-time PCR specific for haemorrhagic septicaemia associated isolates. Veterinary Microbiology 170(3): 335–41.

Pors S E, Hansen M S, Christensen H, Jensen H E, Petersen A and Bisgaard M. 2011. Genetic diversity and associated

pathology of Pasteurella multocida isolated from porcine pneumonia. Veterinary Microbiology 150(3): 354–61.

Sarangi L N, Thomas P, Gupta S K, Kumar S, Viswas K N and Singh V P. 2016. Molecular epidemiology of Pasteurella

multocida circulating in india by multilocus sequence typing. Transboundary and Emerging Diseases 63(2): e286–92.

Shivachandra S B, Viswas K N and Kumar A A. 2011. A review of hemorrhagic septicemia in cattle and buffalo. Animal Health Research Reviews 12(1): 67–82.

Townsend K M, Boyce J D, Chung J Y, Frost A J and Adler B. 2001. Genetic Organization of Pasteurella multocida cap Loci and development of a multiplex capsular PCR typing system. Journal of Clinical Microbiology 39(3): 924–29.

Townsend K M, Frost A J, Lee C W, Papadimitriou J M and Dawkins H J S. 1998. Development of PCR assays for speciesand type-specific identification of Pasteurella multocida isolates. Journal of Clinical Microbiology 36(4): 1096–100.