Association of single nucleotide polymorphism in leptin gene with growth traits of Jamunapari goat

MAHESH SHIVANANAD DIGE; RAKESH KAUSHIK; CHINMOY MISHRA; MAHIMA VERMA; RVS PAWAIYA; SAKET BHUSAN; PRAMOD KUMAR ROUT

doi:10.56093/ijans.v91i11.118133

Authors

MAHESH SHIVANANAD DIGE ICAR-Central Institute of Goat Research, Makhdum, Mathura, Uttar Pradesh 281 122 India
RAKESH KAUSHIK ICAR-Central Institute of Goat Research, Makhdum, Mathura, Uttar Pradesh 281 122 India
CHINMOY MISHRA College of Veterinary Science and Animal Husbandry, Odisha University of Agriculture and Technology, Bhubaneswar, Odisha
MAHIMA VERMA ICAR-Central Institute of Goat Research, Makhdum, Mathura, Uttar Pradesh 281 122 India
RVS PAWAIYA ICAR-Central Institute of Goat Research, Makhdum, Mathura, Uttar Pradesh 281 122 India
SAKET BHUSAN ICAR-Central Institute of Goat Research, Makhdum, Mathura, Uttar Pradesh 281 122 India
PRAMOD KUMAR ROUT ICAR-Central Institute of Goat Research, Makhdum, Mathura, Uttar Pradesh 281 122 India

https://doi.org/10.56093/ijans.v91i11.118133

Keywords:

Association, Goat, Growth, Jamunapari, Leptin, Single nucleotide polymorphism

Abstract

The current study sought to identify single nucleotide polymorphisms (SNPs) in the leptin gene and investigate their relationship with growth traits in goats. Phenotypic data for birth weight (BW), weaning weight (WW), body weight at six month (6MW), body weight at nine month (9MW) and body weight at twelve month (12MW) from Jamunapari goats were recorded. Seven pairs of primers were designed to amplify the leptin gene spanning promoter, exonic, intronic and 3â€™ UTR regions by polymerase chain reaction. High Resolution Melting (HRM) was employed for genotyping, and DNA sequencing was utilized to mining SNPs. Four novel SNPs at g.584C>T, g.4226T>C, g.4158T>C and g.4133T>A were identified in promoter region and 3' UTR region of leptin gene. Statistical analysis revealed that SNPs at g.584C>T was associated with weaning and post weaning stages of growth. Moreover, goats with the CT genotype had higher body weight at WW, 6MW, 9MW and 12MW than those with CC genotype. The results of this study demonstrated that SNPs in leptin gene was significantly associated with growth traits in goat. These findings suggest that an SNP in the caprine leptin gene could be one of the candidates for improving body weight in the Jamunapari goat.

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