Leucaena leucocephala leaves and prickly pear (Opuntia ficus-indica) as substitutes for alfalfa hay (Medicago sativa L.) and their effects on the gas and methane production, prevalence of methanogens and patterns of ruminal fermentation in vitro

JESUS BERNARDO PÁEZ LERMA; MANUEL MURILLO ORTIZ; KARINA AIDE ARAIZA PONCE; SANDRA CONSUELO MARTINEZ ESTRADA; NICOLAS OSCAR SOTO CRUZ; J NATIVIDAD GURROLA REYES

doi:10.56093/ijans.v91i11.118158

Authors

JESUS BERNARDO PÁEZ LERMA National Technological Institute of Mexico
MANUEL MURILLO ORTIZ Faculty of Veterinary Medicine and Zootechnics, Juarez University of the State of Durango
KARINA AIDE ARAIZA PONCE Faculty of Veterinary Medicine and Zootechnics, Juarez University of the State of Durango
SANDRA CONSUELO MARTINEZ ESTRADA National Polytechnic Institute, Mexico
NICOLAS OSCAR SOTO CRUZ Instituto PolitÃ©cnico Nacional, CIIDIR-Unidad Durango. Sigma 119. Durango, MÃ©xico. 34220
J NATIVIDAD GURROLA REYES National Polytechnic Institute, Mexico

https://doi.org/10.56093/ijans.v91i11.118158

Keywords:

In vitro fermentation, Leucaena leucocephala, Methane, Prickly pear

Abstract

The aim of the study was to evaluate the replacement of alfalfa hay with Leucaena leucocephala leaves and prickly pear (Opuntia ficus-indica) as an alternative forage, on gas and methane production, ruminal fermentation patterns and total amount of methanogens during in vitro fermentation. Four treatments were evaluated: (T1) alfalfa hay + concentrate (50 : 50%, DM); (T2) alfalfa hay + Leucaena leaves + concentrate (30 : 20 : 50%, DM); (T3) alfalfa hay + prickly pear + concentrate (30 : 20 : 50%, DM) and (T4) alfalfa hay + Leucaena leaves + prickly pear + concentrate (30 : 10 : 10 : 50%, DM). The higher gas production from of soluble fraction (A) and rate gas production (Kd) values, ammonia-N (NH3-N) concentrations and propionate were found in T1 and lower in T2 (p<0.05). Nevertheless, the inclusion of Leucaena leucocephala in the diet decreased the methane production and did not affect the total of methanogens. Results showed that the replacement of alfalfa hay by Leucaena leucocephala and Opuntia ficus-indica in a diet do not affect the main variables of in vitro gas production, ruminal fermentation patterns and methanogenic microorganisms populations.

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