Evaluation of Bone Morphogenetic Protein-4 gene polymorphism for growth traits in Indian goat breeds
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Authors
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VAISHALI KHARE
Nanaji Deshmukh Veterinary Science University, Jabalpur, Madhya Pradesh 482 001 India
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SHRIKANT JOSHI
Nanaji Deshmukh Veterinary Science University, Jabalpur, Madhya Pradesh 482 001 India
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MOHAN SINGH THAKUR
Nanaji Deshmukh Veterinary Science University, Jabalpur, Madhya Pradesh 482 001 India
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AJIT PRATAP SINGH
Nanaji Deshmukh Veterinary Science University, Jabalpur, Madhya Pradesh 482 001 India
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RAJESH VANDRE
Nanaji Deshmukh Veterinary Science University, Jabalpur, Madhya Pradesh 482 001 India
Keywords:
BMP-4, Goat, Growth traits, PCR-SSCPAbstract
The genetic improvement of production traits can be made through marker assisted selection using a candidate
gene approach. Bone morphogenetic proteins (BMPs) are multifunctional growth factors that belong to the
transforming growth factor b (TGF-b) superfamily. This study aimed to detect the genetic polymorphism of BMP-4
in different goat breeds by polymerase chain reaction-single strand confirmational polymorphism (PCR-SSCP) and
association of polymorphic variants with growth traits. The amplified fragments of BMP4 gene of 380 bp size were
analysed using SSCP in non-denaturing PAGE and the results showed the presence of two genotypes: AA (47-
67%) and BB (33-53%) in Barbari, Sirohi and Black Bengal breed. The association of BMP-4 polymorphism with
different growth trait parameters showed non-significant effect of genotypes. However, some genotypes showed
non-significant superiority over others. Further research on a large population is required to validate the role of the
BMP-4 gene in goat growth traits.
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