Copro-prevalence of Cryptosporidium in pigs of selected districtsin West coast of India: A preliminary study

CHETHAN KUMAR  H B; CHANDISHA  D S; SOLOMON  RAJKUMAR; SHIVASHARANAPPA  N; SUSITHA  R; UDHARWAR  S V; CHAKURKAR  E B

doi:10.56093/ijans.v93i12.119435

Authors

CHETHAN KUMAR H B ICAR-National Institute of Veterinary Epidemiology and Disease Informatics, Yelahanka, Bengaluru, Karnataka 560 064 India
CHANDISHA D S Wad-wad curca, Tiswadi, Goa
SOLOMON RAJKUMAR ICAR-Central Coastal Agricultural Research Institute, Old Goa, Goa
SHIVASHARANAPPA N ICAR-National Institute of Veterinary Epidemiology and Disease Informatics, Yelahanka, Bengaluru, Karnataka 560 064 India
SUSITHA R ICAR-Central Coastal Agricultural Research Institute, Old Goa, Goa
UDHARWAR S V Krishi Vigyan Kendra, Old Goa, Goa
CHAKURKAR E B ICAR-Central Island Agricultural Research Institute, Bathubasti, Port Blair

DOI:

https://doi.org/10.56093/ijans.v93i12.119435

Keywords:

Cryptosporidium, India, Pigs, Prevalence, West coast

Abstract

In India, Cryptosporidium spp. have been detected and characterised from humans and domestic animals, mostly ruminants. Although pigs can act as an important reservoir of Cryptosporidium infection to humans, comprehensive studies have not been conducted in pig population in India. Hence, present study attempt to elucidate the prevalence and diversity of Cryptosporidium infection in pigs in the selected districts of three states situated in the West coast of India. The pig faecal samples (n=221) were subjected to coprological examination using modified Ziehl Neelsen (mZN) staining method, followed by polymerase chain reaction to detect Cryptosporidium spp. Using mZN staining method, 1.35% (95% CI, 0.46%–3.91%) of the samples were found positive for Cryptosporidium oocyst. However, the DNA from Cryptosporidium positive faecal samples could not be amplified using polymerase chain reaction probably because of the low sensitivity of the PCR and or low oocyst number in the faecal sample. Cryptosporidium spp. of pig origin have zoonotic potential and a certain proportion of pigs infected with Cryptosporidium may be apparently healthy. Therefore, the pig farmers need to be made aware of hygienic practices while handling pigs and pig manure and and general public must be made sensitised about the good agricultural practices and standard food hygiene practices to prevent foodborne Cryptosporidium infection.

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