Effect of Azolla cristata with or without enzyme supplementation on blood biochemistry and intestinal histomorphology of broiler chicken

S ADIL; SHOUKAT ARA; M A WANI; M T BANDAY; S A KAMIL

doi:10.56093/ijans.v92i9.120118

Authors

S ADIL Sher-e-Kashmir University of Agricultural Sciences and Technology, Kashmir, Srinagar, Jammu and Kashmir 190 006 India
SHOUKAT ARA Sher-e-Kashmir University of Agricultural Sciences and Technology, Kashmir, Srinagar, Jammu and Kashmir 190 006 India
M A WANI Sher-e-Kashmir University of Agricultural Sciences and Technology, Kashmir, Srinagar, Jammu and Kashmir 190 006 India
M T BANDAY Sher-e-Kashmir University of Agricultural Sciences and Technology, Kashmir, Srinagar, Jammu and Kashmir 190 006 India
S A KAMIL Sher-e-Kashmir University of Agricultural Sciences and Technology, Kashmir, Srinagar, Jammu and Kashmir 190 006 India

https://doi.org/10.56093/ijans.v92i9.120118

Keywords:

Azolla, Blood biochemistry, Broiler chicken, Enzyme, Histomorphology

Abstract

A study was undertaken to determine the effect of partially replacing soybean protein with Azolla (AZ) with or without enzyme on blood biochemistry and intestinal histomorphology of broiler chicken. The experiment was performed on 210 Cobb broiler chicks randomly assigned to 7 treatment groups each replicated 3 times with 10 birds in each replicate. Basal diet was offered to the control (T1) group, whereas soybean protein was replaced with 5 (T2), 10 (T3) and 15% (T4) AZ and the other three groups, i.e. T5, T6 and T7 contained the respective inclusion levels of AZ, but were supplemented with cocktail enzyme. The results revealed that there was no statistical difference on blood glucose, cholesterol, protein, SGPT and SGOT levels among various treatment groups including control. Also there was a no significant effect in villus height (V), crypt depth (C) and V/C ratio among various treatment groups when compared to control. Highest villus height was observed in the control group (T₁) and there was reduction in villus height in all the groups wherein AZ was included in the diet either alone or with enzyme supplementation. It is thus concluded that dietary inclusion of AZ had no adverse effect on the blood biochemistry and intestinal histomorphology when provided alone or in combination with enzyme supplementation, indicating that AZ could be included up to 15% level in the diet of broiler chicken without any adverse effect.

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References

Ara S, Adil S, Ahmad M, Banday M T and Rather A Q. 2012. Effect of Azolla on small intestine of broiler chicken. Indian Journal of Poultry Science 47(2): 241–43.

Ara S, Adil S and Khan M A. 2018. Effect of aquatic fern, Azolla cristata in diet on growth, serum biochemistry and laying performance of chicken. Pakistan Journal of Zoology 50(6): 2325–29. DOI: https://doi.org/10.17582/journal.pjz/2018.50.6.2325.2329

Awad W, Ghareeb K and Bӧhm J. 2008. Intestinal structure and function of broiler on diets supplemented with a synbiotic

containing Enterococcus faecium and oligosaccharides. International Journal of Molecular Science 9: 2205–160.

Bhaskaran K S and Kannapan P. 2015. Nutritional composition of four different species of Azolla. Pelagia Research Library 5(3): 6–12.

Duncan D B. 1955. Multiple range and F tests. Biometrics 11: 1–42. DOI: https://doi.org/10.2307/3001478

Lillie R D. 1965. Histopathological Technique and Practical Histochemistry. New York: Blakiston Div., McGraw-Hills.

Meena G S, Dhaka B L, Singh B, Meena R K and Meena K C. 2017. Effect of Azolla as feed supplement on milk yield in buffaloes. International Journal of Current Microbiology and Applied Science 6(12): 3490–94. DOI: https://doi.org/10.20546/ijcmas.2017.612.406

Parthasarathy R, Kadirvel R and Kathaperumal V. 2002. Azolla as a partial replacement for fishmeal in broiler rations. Indian Veterinary Journal 79: 144–46.

Rana D, Katoch S, Mane B G, Rani D and Sankhyan V. 2017. Carcass characteristic and physico-chemical properties of broiler chicken meat supplemented with Azolla pinnata. Journal of Animal Research 7(6): 1035–41. DOI: https://doi.org/10.5958/2277-940X.2017.00155.3

Saikia N, Sapcota D and Hazarika R. 2014. Effect of feeding Azolla (Azolla pinnata) meal to broilers: A field study in Assam. Indian Journal of Poultry Science 49: 113–14.

Shital S G, Fernandes A P, Darade R V and Zagade B G. 2012. Effect of different levels of Azolla meal on growth performance of Osmanabadi kids. Research Journal of Animal Husbandry and Dairy Science 3: 13–16.