Report on first confirmed outbreaks of contagious agalactia in small ruminants from Maharashtra State of India

VITTHAL  DHAYGUDE; ABHIJIT  BARATE; BHUPESH  KAMDI; JAYANT  SUKARE; DIVYA  SABHARWAL; ASHISH  BHAGWAT; PRASHANT  PAWAR; DUSHYANT  MUGLIKAR

doi:10.56093/ijans.v92i5.120217

Authors

VITTHAL DHAYGUDE Krantisinh Nana Patil College of Veterinary Science, Satara, Maharashtra 412 801 India
ABHIJIT BARATE Krantisinh Nana Patil College of Veterinary Science, Satara, Maharashtra 412 801 India
BHUPESH KAMDI Krantisinh Nana Patil College of Veterinary Science, Satara, Maharashtra 412 801 India
JAYANT SUKARE Krantisinh Nana Patil College of Veterinary Science, Satara, Maharashtra 412 801 India
DIVYA SABHARWAL Krantisinh Nana Patil College of Veterinary Science, Satara, Maharashtra 412 801 India
ASHISH BHAGWAT Krantisinh Nana Patil College of Veterinary Science, Satara, Maharashtra 412 801 India
PRASHANT PAWAR Krantisinh Nana Patil College of Veterinary Science, Satara, Maharashtra 412 801 India
DUSHYANT MUGLIKAR Krantisinh Nana Patil College of Veterinary Science, Satara, Maharashtra 412 801 India

https://doi.org/10.56093/ijans.v92i5.120217

Keywords:

Contagious agalactia, M. agalactiae, PCR, sequencing

Abstract

Mycoplasma agalactiae causes contagious agalactia, a serious, economically important and OIE listed disease
syndrome, in sheep and goats. There are no reports of this disease from Maharashtra state of India. The aim of
the present investigation was to record epidemiological information and to detect M. agalactiae in clinical samples
from natural outbreaks of contagious agalactia. Clinically, anorexia, reluctance to follow the herd, lethargy and fever
were the initial clinical signs noted. Later, mastitis, arthritis and keratoconjunctivitis were noted as important clinical
signs in both sheep and goats. However, all signs were not present in every case. DNA was extracted from mastitic
milk and articular exudate collected from suspected cases and PCR was performed using primers specific for 16S rRNA gene of Mycoplasma spp. The agarose gel electrophoresis of PCR product revealed the presence of bands of expected size (278 bp) indicating positive amplification. The nucleotide sequence analysis of PCR amplicons showed 100% identity with 16S rRNA gene of Mycoplasma agalactiae isolates JF4428 (Accession No. LT578418.1). The outbreaks of contagious agalactia were confirmed for the first time in Maharashtra state of India and carries epidemiological significance.

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