Esterase mediated pyrethroid resistance in Rhipicephalus microplus ticks collected from north-western Himalayas
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Authors
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R GODARA
Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu, Jammu and Kashmir 181 102 India
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R KATOCH
Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu, Jammu and Kashmir 181 102 India
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A YADAV
Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu, Jammu and Kashmir 181 102 India
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KAIFA NAZIM
Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu, Jammu and Kashmir 181 102 India
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OMER MOHI U DIN SOFI
Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu, Jammu and Kashmir 181 102 India
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P K VERMA
Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu, Jammu and Kashmir 181 102 India
Keywords:
Carboxylesterase, Himalayas, Pyrethroids, Resistance, Rhipicephalus microplusAbstract
Larval packet test (LPT) was used to evaluate resistancestatus against deltamethrin and cypermethrin in R. microplusticks collected from 8 districts of Jammu region. Resistanceagainst deltamethrin was detected at level 1 in three isolates,level 2 in two isolates and level 3 in one isolate while twoisolates were found susceptible. Four isolates were foundresistant to cypermethrin whereas, other four isolates weresusceptible. Quantitative analysis of general esteraseactivity revealed a range of 2.466±0.29 to 8.908±1.68 and1.279±0.22 to 5.793±1.24 μmol/min/mg protein for α- andβ-esterase activity, respectively. Multiple pairwisecomparisons of the mean values with susceptible fieldisolate revealed significant elevated levels of both α- andβ-esterases in Jammu isolate having highest RFs to bothdeltamethrin and cypermethrin. It can be concluded thatthe resistance against SPs has developed in R. microplusticks of north-western Himalayan region, India and esterasesplay a significant role in conferring the SPs resistance.Downloads
References
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