Isolation and characterization of sulphate reducing bacteria from goat rumen and its inclusion to improve in vitro feed fermentation

SANDEEP UNIYAL; LAL CHAND CHAUDHARY; ANJU KALA; NEETA AGARWAL

doi:10.56093/ijans.v92i1.120932

Authors

SANDEEP UNIYAL ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India
LAL CHAND CHAUDHARY ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India
ANJU KALA ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India
NEETA AGARWAL ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India

https://doi.org/10.56093/ijans.v92i1.120932

Keywords:

Dissimilatory sulphite reductase gene, Goat rumen, In vitro feed fermentation, Methane, Sulphate reducing bacteria

Abstract

In the present study sulphate-reducing bacteria (SRB) were isolated from the rumen of goats fed a diet of wheat straw and concentrate in 50:50 ratio using specific medium followed by phenotypic and phylogenetic characterization. Based on the biochemical characteristics, four best SRB isolates were evaluated for their efficacy to reduce in vitro CH4 production and stimulate fibre digestion. It was found that true dry matter digestibility (IVTD) and production of metabolites were higher but methane emission was low by inclusion of live culture of SRB4 isolate as compared to control. Sequencing of 16S rDNA revealed 99% homology of SRB4 with Streptococcus caviae strain NR156902. The isolate also exhibited expression of dissimilatory sulphite reductase gene (dsR) gene substantiating sulphate reducing ability of the isolate. The results indicate the ability of SRB4 to reduce in vitro CH4 emissions and improve fibre digestibility, hence can be explored as an effective candidate for microbial feed additive to modify rumen fermentation, so that enteric methane production can be controlled.

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References

Agarwal N, Kamra D N, Chatterjee P N, Kumar R and Chaudhary L C. 2008. In vitro methanogenesis, microbial profile and fermentation of green forages with buffalo rumen liquor as influenced by 2-bromoethane sulphonic acid. Asian Australasian Journal of Animal Science 21: 818–23. DOI: https://doi.org/10.5713/ajas.2008.70336

An D, Dong X and Dong Z. 2005. Prokaryote diversity in the rumen of yak (Bos grunniens) and Jinnan cattle (Bos taurus) estimated by 16S rDNA homology analyses. Anaerobe. 11: 207–15. DOI: https://doi.org/10.1016/j.anaerobe.2005.02.001

Beeman R E and Suflita J M. 1987. Microbial ecology of a shallow unconfined ground water aquifer polluted by municipal landfill leachate. Microbiology Ecology 14: 39–54. DOI: https://doi.org/10.1007/BF02011569

Coleman G S. 1960. A sulphate-reducing bacterium from the sheep rumen. Journal of General Microbiology 22: 423–36. DOI: https://doi.org/10.1099/00221287-22-2-423

Cottyn B G and Boucque C V. 1968. Rapid method for the gas chromatographic determination of volatile fatty acids in rumen fluid. Journal of Agriculture and Food Chemistry 16: 105– 07. DOI: https://doi.org/10.1021/jf60155a002

Dröge S, Limper U, Emtiazi F, Schönig I, Pavlus N, Drzyzga O, Fischer U and König H. 2005. In vitro and in vivo sulphate reduction in the gut contents of the termite Mastotermes darwiniensis and the rose-chafer Pachnoda marginata. Journal of General and Applied Microbiology 51: 57–64. DOI: https://doi.org/10.2323/jgam.51.57

Ellis J E, McIntyre P S, Saleh M, Williams A G and Lloyd D. 1991. Influence of CO2 and low concentrations of O2 on fermentative metabolism of the ruminal ciliate Polyplastron multivesiculatum. Applied and Environmental Microbiology 57: 1400–07. DOI: https://doi.org/10.1128/aem.57.5.1400-1407.1991

Gibson G R, Macfariane G T and Cummings J H. 1993. Sulphate reducing bacteria and hydrogen metabolism in the large intestine. Gut 34: 437–39. DOI: https://doi.org/10.1136/gut.34.4.437

Howard B H and Hungate R E. 1976. Desulfovibrio of the sheep rumen. Applied and Environmental Microbiology 32: 598–602. Huisingh J, McNeill J J and Matrone G. 1974. Sulfate reduction by a Desulfovibrio species isolated from sheep rumen. Applied Microbiology 28: 489–97. DOI: https://doi.org/10.1128/aem.32.4.598-602.1976

ICAR. 2013. Nutrient Requirements of Sheep and Goats. 3rd edn. Indian Council of Agricultural Research. New Delhi, India

Janssen P H and Kirs M. 2008. Structure of the archaeal community of the rumen. Applied and Environmental Microbiology 74: 3619–25. DOI: https://doi.org/10.1128/AEM.02812-07

Jeyanathan J, Martin C and Morgavi D P. 2014. The use of direct- fed microbials for mitigation of ruminant methane emissions: a review. Animal 8: 250–61. DOI: https://doi.org/10.1017/S1751731113002085

Kondo R, Nedwell David B, Purdy Kevin J and Silva S Q. 2004. Detection and enumeration of sulphate-reducing bacteria in estuarine sediments by competitive PCR. Geomicrobiology Journal 21: 145–57. DOI: https://doi.org/10.1080/01490450490275307

Kondo R, Mori Y and Sakami T. 2012. Comparison of sulphate- reducing bacterial communities in Japanese fish farm sediments with different levels of organic enrichment. Microbes and Environments 27(2): 193–99. DOI: https://doi.org/10.1264/jsme2.ME11278

Kumari S, Dahiya R P, Naik S N, Hiloidhari M, Thakur I S, Sharawat I and Kumari N. 2016. Projection of methane emissions from livestock through enteric fermentation: A case study from India. Environmental Development 20: 31–44. DOI: https://doi.org/10.1016/j.envdev.2016.08.001

Lakhani N, Kamra D N, Kala A, Agarwal N, Chaudhary L C and Chaturvedi V B. 2019. Effects of dietary supplementation with rumen modifier and sodium sulphate on methane production and performance of buffalo calves. Animal Nutrition and Feed Technology 19: 169–80. DOI: https://doi.org/10.5958/0974-181X.2019.00016.7

McSweeney C S and Denman S E. 2007. Effect of sulfur supplements on cellulolytic rumen micro-organisms and microbial protein synthesis in cattle fed a high fibre diet. Journal of Applied Microbiology 103: 1757–65. DOI: https://doi.org/10.1111/j.1365-2672.2007.03408.x

Menke K H and Steingass H. 1988. Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid. Animal Research Development 28: 7–55.

Paul S S, Deb S M and Singh D. 2011. Isolation and characterization of novel sulphate-reducing Fusobacterium sp. and their effects on in vitro methane emission and digestion of wheat straw by rumen fluid from Indian riverine buffaloes. Animal Feed Science Technology 167: 132–40. DOI: https://doi.org/10.1016/j.anifeedsci.2011.04.062

Prasada Babu G, Subramanyam P, Sreenivasulu B and Paramageetham C H. 2014. Isolation and identification of sulfate reducing bacterial strains indigenous to sulphur rich barite mines. International Journal of Current Microbiology and Applied Science 3: 788–93

Snedecor G W and Cochran W G. 1994. Statistical Method, 6th edn. The Iowa State University Press, Lowa, USA.

Tkachuk N, Zelena L, Mazur P and Lukash O. 2020. Genotypic, physiological and biochemical features of Desulfovibrio strains in a sulfidogenic microbial community isolated from the soil of ferrosphere. Ecological Questions 31(2): 79–88. DOI: https://doi.org/10.12775/EQ.2020.016

Uniyal S, Chaudhary L C, Kala A, Agarwal N and Kamra D N. 2020. Effect of sulphur supplementation on methane emission, energy partitioning and nutrient utilization in goats. Animal Nutrition and Feed Technology 20: 111–19. DOI: https://doi.org/10.5958/0974-181X.2020.00011.6

Van Soest P J, Robertson J B and Lewis B A. 1991. Methods for dietary fiber, nuteral ditergent fiber and non-starch polysaccharides in relation to animal nutrition. Journal of Dairy Science 74: 3583–97. DOI: https://doi.org/10.3168/jds.S0022-0302(91)78551-2

Wagner M A, Roger J, Flax J L, Brusseau G A and Stahl D A. 1998. Phylogeny of dissimilatory sulfite reductases supports an early origin of sulfate respiration. Journal of Bacteriology 180: 2975–82. DOI: https://doi.org/10.1128/JB.180.11.2975-2982.1998

Wheatherburn M W. 1967. Phenol-hypochlorite reaction for determination of ammonia. Analytical Chemistry 39: 971– 74. DOI: https://doi.org/10.1021/ac60252a045

Yatoo M A, Chaudhary L C, Agarwal N, Chaturvedi V B and Kamra D N. 2018. Effect of feeding of blend of essential oils on enteric methanogenesis, growth, nutrient utilization in growing buffaloes. Asian-Australasian Journal of Animal Sciences 31: 672–76. DOI: https://doi.org/10.5713/ajas.16.0508

Yu Z and Morrison M. 2004. Improved extraction of PCR-quality community DNA from digesta and fecal samples. Biotechniques 36: 808–12. DOI: https://doi.org/10.2144/04365ST04