Detection of F94L marker in myostatin (MSTN/TaqI) gene of Indonesian Sumba Ongole cattle (Bos indicus)
Abstract views: 44 / PDF downloads: 61
Keywords:F94L marker, MSTN/TaqI gene, Sumba Ongole (SO) cattle
Sumba Ongole (SO) cattle are Ongole cattle from India which were imported to Indonesia during the Dutch colonial era and were well adapted on Sumba Island, East Nusa Tenggara, Indonesia. Sumba Ongole (SO) cattle are known for high meat production. This research was conducted to identify one point mutation of g.415C/A (F94L marker) in the myostatin (MSTN) gene (exon 1) using PCR-RFLP method with TaqI restriction enzyme (T*CGA). DNA samples of SO bulls and cows (Total 153) from Sumba Island were used in this study. Results revealed that all samples in this study had CC genotype with C allele as the common allele. The mutation point of g.415C/A was not found in this study. However, one mutation point of g.400G/A was detected in this study without amino acid changes (synonymous). It was concluded that MSTN/TaqI gene in the present study is monomorphic and cannot be used as the genetic marker for productive traits of SO cattle.
Abe T, Hasebe H, Okumura T, Kuchida K, Kawamura T and Kobayashi E. 2009. Detection of DNA polymorphisms in the MSTN gene and the effect of F94L on meat quantity and quality traits in Japanese Black × Limousin F2 population. Journal of Animal and Veterinary Advances 8(8): 1609–15.
Alexander L J, Kuehn L A, Smith T P L, Matukumalli L K, Mote B, Koltes J E, Reecy J, Geary T W, Rule D C and MacNeil M D. 2009. A Limousin specific myostatin allele affects longissimus muscle area and fatty acid profiles in a Wagyu-Limousin F2 population. Journal of Animal Science 87(5): 1576–81. DOI: https://doi.org/10.2527/jas.2008-1531
Anwar S, Volkandari S D, Wulandari A S, Putra W P B, Sophian E and Said S. 2020. Detection of F94L mutation of the MSTN gene in four Indonesian local cattle breeds. Journal of the Indonesian Tropical Animal Agriculture 45(1): 7–14. DOI: https://doi.org/10.14710/jitaa.45.1.7-14
Charlier C, Coppieters W, Farnir F, Grobet L, Leroy P L, Michaux C, Mni M, Schwers A, Vanmanshoven P, Hanset R and Georges M. 1995. The mh gene causing double-muscling in cattle maps to bovine Chromosome 2. Mammalian Genome 6(11): 788–92. DOI: https://doi.org/10.1007/BF00539005
Curi R A, Krauskopf M M, Hadlich J C, Fortes M R S, Vankan D M, Silva J A V, de Oliveira H N and da Mota M D S. 2012. Candidate SNP’s for carcass and meat traits in Nelore animals and in their crosses with Bos taurus. Pesquisa Agropecuaria Brasileira 47(2): 294–301. DOI: https://doi.org/10.1590/S0100-204X2012000200019
Cushman R A, Jr Trait R G, McNeel A K, Forbes E D, Amundson O L, Lents C A, Lindholm-Perry A K, Wood J R, Cupp A S, Smith T P L, Freetly H C and Bennett G L. 2015. A polymorphism in myostatin influences puberty but not fertility in beef heifers, whereas μ-calpain affects first calfbirth weight. Journal of Animal Science 93(1): 117–26. DOI: https://doi.org/10.2527/jas.2014-8505
Esmailizadeh A K, Bottema C D K, Sellick G S, Verbyla A P, Morris C A, Cullen N G and Pitchford W S. 2008. Effects of the myostatin F94L substitution on beef traits. Journal of Animal Science 86(5): 1038–46. DOI: https://doi.org/10.2527/jas.2007-0589
Gholamhoseini G F, Mohammadabadi M R and Asadi F M 2018. Polymorphism of the growth hormone gene and its effect on production and reproduction traits in goat. Iranian Journal of Applied Animal Science 8(4): 653–59.
Gooki F G, Mohammadabadi M, Fozi M A and Soflaei M. 2019. Association of biometric traits with growth hormone gene diversity in raini cashmere goats. Walailak Journal of Science and Technology (WJST) 16(7): 499–508. DOI: https://doi.org/10.48048/wjst.2019.3791
Hartati Y T, Utsunomiya, Tad S, Sonstegard, José F G, Jakaria and Muladno. 2015. Evidence of Bos javanicus × Bos indicus hybridization and major QTLs for birth weight in Indonesian Peranakan Ongole cattle. BMC Genetics. DOI: https://doi.org/10.1186/s12863-015-0229-5
Kumar M, Ratwan P and Dahiya S P. 2020. Potential candidate gene markers for milk fat in bovines: A review. Indian Journal of Animal Sciences 90(5): 667–71.
Mohammadabadi M R, Soflaei M, Mostafavi H and Honarmand M. 2011. Using PCR for early diagnosis of bovine leukemia virus infection in some native cattle. Genetics and Molecular Research 10(4): 2658–63. DOI: https://doi.org/10.4238/2011.October.27.2
Mohammadabadi M R. 2021. Tissue-specific mRNA expression profile of ESR2 gene in goat. Agricultural Biotechnology Journal 12(4): 169–84.
Nei M and Kumar S. 2000. Molecular Evolution and Phylogenetics. Oxford University Press, New York.
Sheng H, Guo Y, Zhang L, Zhang J, Miao M, Tan H, Hu D, Li X, Ding X, Li G and Guo H. 2021. Proteomic studies on the mechanism of myostatin regulating cattle skeletal muscle development. Frontiers in Genetics 12: 1–18. DOI: https://doi.org/10.3389/fgene.2021.752129
Smith J A, Lewis A M, Wiener P and Williams J L. 2000. Genetic variation in the bovine myostatin gene in UK beef cattle: Allele frequencies and haplotype analysis in the South Devon. Animal Genetics 31(5): 306–09. DOI: https://doi.org/10.1046/j.1365-2052.2000.00521.x
Smith T P, Lopez-Corrales N L, Kappes S M and Sonstegard T S. 1997. Myostatin maps to the intervalcontaining the bovine mh locus. Mammalian Genome 8(10): 742–44. DOI: https://doi.org/10.1007/s003359900557
Tantia M S, Vijh R K, Kumar S T B, Mishra B and Reecy J M. 2006. Comparative analysis of GDF 8 (myostatin) in Bos indicus and Bos taurus. DNA Sequence 17(4): 311–13. DOI: https://doi.org/10.1080/10425170600807603
Vankan D M, Waine D and Fortes M. 2010. Real-time PCR genotyping and frequency of the myostatin F94L mutation in beef cattle breeds. Animal 4(4): 530–34. DOI: https://doi.org/10.1017/S175173110999139X
Wu D, Gu M, Wei Z, Bai C, Su G, Liu X, Zhao Y, Yang L and Li G. 2022. Myostatin knockout regulates bile acid metabolism by promoting bile acid synthesis in cattle. Animals 12(2): 1–13. DOI: https://doi.org/10.3390/ani12020205
How to Cite
The copyright of the articles published in The Indian Journal of Animal Sciences vests with the Indian Council of Agricultural Research, who has the right to enter into any agreement with any organization in India or abroad engaged in reprography, photocopying, storage and dissemination of information contained in these journals. The Council has no objection in using the material, provided the information is being utilized for academic purpose but not for commercial use. Due credit line should be given to the ICAR where information will be utilized.