Novel Cold-Inducible RNA-Binding Protein (CIRBP) in goat spermatozoa: A possible cryo-stress reliever during the process of Cryopreservation
![](/public/icon/gor.png)
![](/public/icon/pdf.png)
Keywords:
Zinc, Copper, 2D Gel, MALDI-TOF, Western blotting, CIRBP, cryopreservation, spermatozoa, bucksAbstract
The great expectation of the dairy owners from the frozen semen industry is quality semen with high fertility. Many researchers concentrate in this area to produce frozen semen of high quality and fertility. However, the problem still exists to minimize or reduce the cryoinjury related damages that occur during the process of cryopreservation with the use of all kinds of semen additives. The success rate of the additives added semen is variable at a larger scale. Therefore, any new molecules which can effectively prevent or minimize the cryoinjury are warranted and it could be low cost substances. Our incidental finding and protein identification with molecular weight ≈ 19 kDa (cold-inducible RNA-binding protein CIRBP) in goat spermatozoa seems to be effective in minimizing cryoinjury. The identified CIRBP was confirmed via Western blotting. In future, further studies are needed to confirm the molecular mechanism of the CIRBP on the cryoinjury as semen additives, and application of this protein added semen in the field to improve the level of conception rate in cattle.
Downloads
References
Arangasamy A, Venkata Krishnaiah M, Manohar N, Selvaraju S, Guvvala P R, Soren N M, Reddy I J, Roy K S and Ravindra J P. 2018. Advancement of puberty and enhancement of seminal characteristics by supplementation of trace minerals to bucks. Theriogenology 110: 182–91.
Arunachalam A, Krishnaiah M V, Manohar N, Selvaraju S, Rani G P, Soren N M, Reddy I J and Ravindra J P. 2018.
Cryoprotective role of organic Zn and Cu supplementation in goats (Capra hircus) diet. Cryobiology 81: 117–24.
Dance A, Thundathil J, Blondin P and Kastelic J. 2016. Enhanced early-life nutrition of Holstein bulls increases sperm
production potential without decreasing postpubertal semen quality. Theriogenology 86: 687–94.e2.
Gromova I and Celis J E. 2006. Protein detection in gels by silver staining: A procedure compatible with mass spectrometry. Cell Biology 4: 219–23.
Hemalatha K, Arangasamy A, Selvaraju S, Krishnaiah M V, Rani G P, Mishra A, Soren N B, Reddy I J and Ravindra J P. 2018. Effect of dietary supplementation of organic zinc and copper on in vitro semen fertility in goat. Small Ruminant Research 161: 68–72.
Hezavehei M, Sharafi M, Kouchesfahani H M, Henkel R, Agarwal A, Esmaeili V and Shahverdi A. 2018. Sperm
cryopreservation: A review on current molecular cryobiology and advanced approaches. Reproductive Biomedicine Online 37: 327–39.
Himanshu B. 2019. Dietary flaxseed and coconut oil supplementation effect on semen profile and hormone level in
adult ram. IVRI, Izatnagar, Bareilly, Uttar Pradesh. Horváth A, Szenci O, Nagy K, Végh L and Pribenszky C. 2016.
Stress preconditioning of semen before cryopreservation improves fertility and increases the number of offspring
born: A prospective randomised study using a porcine model. Reproduction, Fertility and Development 28: 475–81.
Masuda T, Itoh K, Higashitsuji H, Hisako H, Nakazawa N, Sakurai T, Liu Y, Tokuchi H, Fujta T, Zhao Y, Nishiyama H, Tanaka T, Fukumoto M, Ikawa M,Okabe M and Fujita J. 2012. Coldinducible RNA-binding protein (Cirp) interacts with Dyrk1b/ Mirk and promotes proliferation of immature male germ cells in mice. Proceedings of the National Academy of Science 109: 10885–90.
Mayasula V K, Arangasamy A, Selvaraju S, Guvvala P R and Ghosh J. 2022. Organic Zinc and Copper supplementationassociated changes in gene expression and protein profiles in Buck spermatozoa. Biological Trace Elements Research 200:1626–39. DOI: https://doi.org/10.1007/s12011-021-02796-x
Narasimhaiah M, Arunachalam A, Sellappan S, Krishnaiah M V, Rani G P, Ghosh S, Chandra V, Ghosh J and Kumar H. 2018.Organic zinc and copper supplementation on antioxidant protective mechanism and their correlation with sperm functional characteristics in goats. Reproduction in Domestic Animals 53: 644–54.
Nishiyama H, Danno S, Kaneko Y, Itoh K, Yokoi H, Fukumoto M, Mill'an J L, Matsuda T, Yoshida O and Fujita J. 1998.
Decreased expression of cold-inducible RNA-binding protein (CIRP) in male germ cells at elevated temperature. American Journal of Pathology 152: 289.
Pribenszky C, Horváth A, Végh L, Huang S-Y, Kuo Y-H and Szenci O. 2011. Stress preconditioning of Boar permatozoa:
A new approach to enhance semen quality. Reproduction in Domestic Animals 46: 26–30.
Qi X L, Xing K, Huang Z, Chen Y, Wang L, Zhang L C, Sheng X H, Wang X G, Ni H M and Guo Y 2020. Comparative transcriptome analysis digs out genes related to antifreeze between fresh and frozen–thawed rooster sperm. Poultry
Science 99: 2841–51.
Roilo M, Kullmann M K and Hengst L. 2018. Cold-inducible RNA-binding protein (CIRP) induces translation of the cellcycle inhibitor p27Kip1. Nucleic Acids Research 46: 3198–210.
Sangeeta S, Arangasamy A, Kulkarni S and Selvaraju S 2015. Role of amino acids as additives on sperm motility, plasma membrane integrity and lipid peroxidation levels at pre-freeze and post-thawed ram semen. Animal Reproduction Science 161: 82–8.
Sekar B, Arangasamy A, Naidu S J, Reddy I J and Bhatta R. 2021. Organic mineral supplementation on differential protein profile of Osmanabadi bucks (Capra hircus). Reproductive Biology 21: 100533. Xia Z, Zheng X, Zheng H, Liu X, Yang Z and Wang X. 2012. Cold-inducible RNA-binding protein (CIRP) regulates target
mRNA stabilization in the mouse testis. FEBS Lett 586: 3299–308.
Yash Pal, A Arangasamy, R A Legha, J Singh, R S Bansal, S K Khurana and Tandon S N. 2011. Freezability and fertility of
Marwari stallion semen. Indian Journal of Animal Science 81:445–92.
Downloads
Submitted
Published
Issue
Section
License
The copyright of the articles published in The Indian Journal of Animal Sciences is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.