Physiological parameters and serum electrolyte changes after betaine supplementation in lactating Murrah buffaloes during hot-humid season

JYOTSANA SHAKKARPUDE; ADITYA MISHRA; DEEPIKA D CAESAR; ANAND K JAIN; SANJU MANDAL; DANVEER S YADAV; ARCHANA JAIN; AAMRAPALI BHIMTE; BHAVNA AHIRWAR

doi:10.56093/ijans.v93i8.123025

Authors

JYOTSANA SHAKKARPUDE Nanaji Deshmukh Veterinary Science University, Jabalpur, Madhya Pradesh 482 004 India
ADITYA MISHRA College of Veterinary Science and Animal Husbandry, Jabalpur, Madhya Pradesh
DEEPIKA D CAESAR College of Veterinary Science and Animal Husbandry, Jabalpur, Madhya Pradesh
ANAND K JAIN College of Veterinary Science and Animal Husbandry, Jabalpur, Madhya Pradesh
SANJU MANDAL College of Veterinary Science and Animal Husbandry, Jabalpur, Madhya Pradesh
DANVEER S YADAV Nanaji Deshmukh Veterinary Science University, Jabalpur, Madhya Pradesh 482 004 India
ARCHANA JAIN Nanaji Deshmukh Veterinary Science University, Jabalpur, Madhya Pradesh 482 004 India
AAMRAPALI BHIMTE Nanaji Deshmukh Veterinary Science University, Jabalpur, Madhya Pradesh 482 004 India
BHAVNA AHIRWAR College of Veterinary Science and Animal Husbandry, Rewa, Madhya Pradesh

https://doi.org/10.56093/ijans.v93i8.123025

Keywords:

Betaine, Buffalo, Rectal temperature, Serum chloride, Serum sodium

Abstract

Betaine acts as a methyl donor in metabolism and serves as an organic osmolyte, which is used by cells for protection against osmotic stress and high temperatures. The present study was carried out to investigate the effect of betaine on physiological parameters and serum electrolyte changes in postpartum lactating Murrah buffaloes during the hot-humid season. The study was performed at the Livestock Farm Complex, N.D.V.S.U., Jabalpur, in 2019. Eighteen postpartum Murrah buffaloes were randomly divided into three groups for the experiment. T1 was a control group. T2 and T3 groups were supplemented with betaine at 50 g/animal/day and 100 g/animal/day, respectively. Betaine was supplemented in the feed from day 5 postpartum and continued up to 4 months. The rectal temperature was found to be significantly lower in the T2 and T3 groups as compared to the T1 group on day 90 postpartum. The pulse rate was lower in the T2 and T3 groups as compared to the control group on day 105 postpartum. It was concluded that betaine improves serum sodium, potassium, and the chloride concentration as compared to the control group and reduces heat stress in lactating buffaloes.

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References

Chambers S T. 1995. Betaines: Their significance for bacteria and the renal tract. Clinical Science 88: 25–27. DOI: https://doi.org/10.1042/cs0880025

Craig S A S. 2004. Betaine in human nutrition. American Journal of Clinical Nutrition 80: 539–49. DOI: https://doi.org/10.1093/ajcn/80.3.539

Cronje P B. 2005. Heat stress in livestock – the role of the gut in its aetiology and a potential role for betaine in its alleviation. Recent Advances in Animal Nutrition in Australia 15: 107–22.

Deshpande A, Singh S V, Somagond Y M, Sheoran P, Naskar S and Chahal V P. 2020. Physio-biochemical responses and growth performance of buffalo heifers to betaine supplementation during hot humid season under field conditions. Indian Journal of Animal Sciences 90(3): 85–92. DOI: https://doi.org/10.56093/ijans.v90i3.102523

DiGiacomo K, Simpson S, Leury B J and Dunshea F R. 2016. Dietary betaine impacts the physiological responses to moderate heat conditions in a dose dependent manner in sheep. Animals 6(51): 1–13. DOI: https://doi.org/10.3390/ani6090051

Dunshea F R, Oluboyede K, DiGiacomo K, Leury B J and Cottrell J J. 2019. Betaine improves milk yield in grazing dairy cows supplemented with concentrates at high temperatures. Animals 9(57): 1–7. DOI: https://doi.org/10.3390/ani9020057

Hall LW, Dunshea F R, Allen J D, Rungruang S, Collier J L, Long N M and Collier R J. 2016. Evaluation of dietary betaine in lactating Holstein cows subjected to heat stress. Journal of Dairy Science 99(12): 9745–53. DOI: https://doi.org/10.3168/jds.2015-10514

ICAR. 2013. Nutrient Requirements of Cattle and Buffaloes. Indian Council of Agricultural Research, New Delhi, India.

Mendoza S M, Boyd R D, Ferket P R and van Heugten E. 2017. Effects of dietary supplementation of the osmolyte betaine on growing pig performance and serological and hematological indices during thermoneutral and heat-stressed conditions. Journal of Animal Science 95(11): 5040–53. DOI: https://doi.org/10.2527/jas2017.1905

NRC. 1971. A guide to environmental research on animals. National Academy of Science Washington, DC.

Petronini P G, De Angelis E M, Borghetti P, Borghetti A F and Wheeler K P. 1992. Modulation by betaine of cellular responses to osmotic stress. Biochemical Journal 282: 69–73. DOI: https://doi.org/10.1042/bj2820069

Raheja N. 2017. ‘Effect of betaine supplementation on reproductive performance of Karan Fries cows during hot humid season.’ M.V.Sc. Thesis (Veterinary Gynaecology and Obstetrics), National Dairy Research Institute, Karnal, India.

Saeed M, Babazadeh D, Naveed M, Arain M A, Hassan F U and Chao S. 2017. Reconsidering betaine as a natural anti-heat stress agent in poultry industry: A review. Tropical Animal Health and Production 49(7): 1329–38. DOI: https://doi.org/10.1007/s11250-017-1355-z

Shankhpal S S, Waghela C R, Sherasia P L, Srivastava A K and Sridhar V. 2018. Betaine supplementation and milk production during heat stress in Crossbred cows. Indian Journal of Animal Nutrition 35(4): 386–90. DOI: https://doi.org/10.5958/2231-6744.2018.00058.0

Shankhpal S S, Waghela C R, Sherasia P L, Srivastava A K and Sridhar V. 2019. Effect of feeding betaine hydrochloride and bypass fat supplement on feed intake, milk yield and physiological parameters in lactating buffaloes during heat stress. Indian Journal of Dairy Science 72(3): 297–301. DOI: https://doi.org/10.33785/IJDS.2019.v72i03.009

Williams S R O, Milner T C, Garner J B, Moate P J, Jacobs J L, Hannah M C, Wales W J and Marett L C. 2021. Dietary fat and betaine supplements offered to lactating cows affect dry matter intake, milk production and body temperature responses to an acute heat challenge. Animals 11(11): 3110. DOI: https://doi.org/10.3390/ani11113110

Zhang L, Ying S J, An W J, Lian H, Zhou G B and Han Z Y. 2014. Effects of dietary betaine supplementation subjected to heat stress on milk performances and physiology indices in dairy cow. Genetics and Molecular Research 13(3): 7577–86. DOI: https://doi.org/10.4238/2014.September.12.25