Hepatoprotective and nephroprotective efficacy of Cichorium intybus following imidacloprid induced subchronic toxicity in WLH cockerels
Abstract views: 153 / PDF downloads: 137
https://doi.org/10.56093/ijans.v92i8.123891
Keywords:
Chicory, Hepatic, Insecticide, Renal, SilymarinAbstract
The current research work was undertaken to evaluate the hepatoprotective and nephroprotective potential of Cichorium intybus following subchronic exposure of imidacloprid in white leghorn (WLH) chicks. Thirty, 6 to 8 weeks old chicks of 300-350 g weight were randomly and equally divided into five groups. Group I served as control and was fed normal grower ration and other were fed medicated ration containing Cichorium intybus leaf powder @ 5000 ppm in Group II, imidacloprid @ 100 ppm in Group III, imidacloprid @ 100 ppm + silymarin @ 100 ppm in Group IV and imidacloprid @ 100 ppm + Cichorium intybus leaf powder @ 5000 ppm in Group V, respectively, for 8 weeks. Biochemical parameters at 4th and 8th week revealed a significant increase in alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, γ-glutamyl transferase, lactate dehydrogenase, total bilirubin, indirect bilirubin, creatinine, blood urea nitrogen (BUN) with an increase in absolute and relative organ weights of liver and kidney after 8 weeks and a significant decline in total protein, albumin and globulin in imidacloprid treated Group III as compared to control (Group I). However, the simultaneous administration of Cichorium intybus leaf powder (CILP) in Group V revealed amelioration in these parameters at par with Groups I and IV. Thus, amelioration of imidacloprid induced hepatotoxic and nephrotoxic effects following simultaneous CILP administration indicates hepatoprotective and nephroprotective potential of Cichorium intybus in imidacloprid intoxicated cockerels.
Downloads
References
Abd-Elhakim Y, Mohammed H H and Mohamed W A. 2018. Imidacloprid impacts on neurobehavioral performance,
oxidative stress, and apoptotic events in the brain of adolescent and adult rats. Journal of Agricultural and Food Chemistry 66(51): 13513–24.
Abu Zeid E H, Alam R T H, Ali S A and Hendawi M Y. 2019. Dose-related impacts of imidacloprid oral intoxication on brain and liver of rock pigeon (Columba livia domestica), residues analysis in diferent organs. Ecotoxicology and Environmental Safety 167: 60–68. DOI: https://doi.org/10.1016/j.ecoenv.2018.09.121
Al-Snafi A E. 2016. Medical importance of Cichorium intybus: A review. IOSR Journal of Pharmacy 6: 41–56. Aly H A, Lightfoot D A and El-Shemy H A. 2009. Modulatory role of lipoic acid on lipopolysaccharide induced oxidative
stress in adult rat sertoli cells in vitro. Chemico-Biological Interactions 182(2): 112–18.
Arfat Y, Mahmood N, Tahir M U, Rashid M, Anjum S, Zhao F, Li D J, Sun Y L, Hu L, Zhihao C and Yin C. 2014. Efect of
imidacloprid on hepatotoxicity and nephrotoxicity in male albino mice. Toxicology Reports 1: 554–61. DOI: https://doi.org/10.1016/j.toxrep.2014.08.004
Badawy M H, Ahmed N S and Attia A M. 2018. Sub-acute oral toxicity of imidacloprid and fipronil pesticide mixture in male albino rats; biochemical and reproductive toxicity evaluation. Journal of Materials and Environmental Science 9(8): 2431–37.
Burtis C A and Ashwood E R. 1999. Tietz Textbook of Clinical Chemistry. 3rd edition. Saunders, Philadelphia. Dufour D R, Lott J A, Nolte F S, Gretch D R and Koff R S. 2001. Diagnosis and monitoring of hepatic injury. II. Recommendations for use of laboratory tests in screening, diagnosis and monitoring. Clinical Chemistry 47: 1133–35.
Edelstein C L. 2008. Biomarkers of acute kidney injury. Advances in Chronic Kidney Disease 15(3): 222–34. DOI: https://doi.org/10.1053/j.ackd.2008.04.003
Elgengaihi S, Mossa A T, Refaie A A and Aboubaker D. 2016. Hepatoprotective efficacy of Cichorium intybus L. extract
against carbon tetrachloride induced liver damage in rats. Journal of Dietary Supplements 13(5): 570–84.
El-Halwagy M E A, Hussein R H, Hamza A H and Al-Bishri W M. 2018. Hepatoprotective effect of alpha lipoic acid versus intoxication with imidacloprid widely used in KSA in albino rats. International Journal of Pharmaceutical Research and Allied Sciences 7(3): 224–32.
Emam H, Emam A and Abdel-Daim M. 2018. Antioxidant capacity of omega-3-fatty acids and vitamin E against imidaclopridinduced hepatotoxicity in Japanese quails. Environmental Science and Pollution Research 25(12): 11694–702. DOI: https://doi.org/10.1007/s11356-018-1481-9
Goldberg D M. 1980. Structural, functional and clinical aspects of γ-Glumatyltransferase. CRC Critical Reviews in Clinical Laboratory Sciences 12(1): 1–58. DOI: https://doi.org/10.3109/10408368009108725
Graham S S, Gail L W and Robin M W. 2013. Clinical pathology in non-clinical toxicology testing. Haschek and Rousseaux’s Handbook of Toxicologic Pathology. 3rd edition. pp 565–94. DOI: https://doi.org/10.1016/B978-0-12-415759-0.00018-2
Gupta M, Singh S P and Pankaj N K. 2014. Evaluation of protective effect of Erythrina variegata and Spirulina
platensis in cockerels intoxicated with imidacloprid. Journal of Veterinary Pharmacology and Toxicology 13(2): 146–51.
Helal E, Samia M, Atef M and Ghada A. 2011. Effect of Cichorium intybus L. on fatty liver induced by oxytetracycline in albino rats. Egyptian Journal of Hospital Medicine 45: 522–35. DOI: https://doi.org/10.21608/ejhm.2011.16381
Jeschke P, Nauen R, Schindler M and Elbert A. 2010. Overview of the status and global strategy for neonicotinoids. Journal of Agricultural and Food Chemistry 59: 2897–2908. Kapoor U, Srivastava M K and Srivastava L P. 2011. Toxicological impact of technical imidacloprid on ovarian morphology, hormones and antioxidant enzymes in female rats. Food and Chemical Toxicology 49: 3086–89. DOI: https://doi.org/10.1016/j.fct.2011.09.009
Keshk W A, Soliman N A, Ali D A and Elseady W S. 2019. Mechanistic evaluation of AMPK/SIRT1/FXR signalling
axis, inflammation and redox status in thioacetamide induced liver cirrhosis: The role of Cichorium intybus L. (chicory) supplemented diet. Journal of Food and Biochemistry 43(8): e12938.
Khalil S R, Awad A, Mohammed H H and Nassan M A. 2017. Imidacloprid insecticide exposure induces stress and disrupts glucose homeostasis in male rats. Environmental Toxicology and Pharmacology 55: 165–74. DOI: https://doi.org/10.1016/j.etap.2017.08.017
Li G Y, Gao H Y, Huang J, Lu J, Gu J K and Wang J H. 2014. Hepatoprotective effect of Cichorium intybus L., a traditional
Uyghur medicine, against carbon tetrachloride-induced hepatic fibrosis in rats. World Journal of Gastroenterology 20: 4753–60. DOI: https://doi.org/10.3748/wjg.v20.i16.4753
Maletha D, Singh S P, Ramanarayanan S, Pandey D and Taj G. 2020. In vitro phytochemical analysis and evaluation
of antioxidant activity of Cichorium intybus. Journal of Veterinary Pharmacology and Toxicology 19(2): 10–13.
Maletha D, Singh S P, Ramanarayanan S, Pandey D and Taj G. 2021. The ameliorating potential of Cichorium intybus against imidacloprid induced intoxication in WLH cockerels. Journal of Veterinary Pharmacology and Toxicology 20(1): 52–56.
Mathur N, Pande K D, Aeri V, Kishore A, Joshi V, Madaan A and Verma R. 2014. Determination of antioxidant and
hepatoprotective ability of flavonoids of Cichorium intybus. International Journal of Toxicological and harmacological
Research 6(4): 107–112.
Mousa M A, Osman A S and Hady H A. 2017. Performance, immunology and biochemical parameters of Moringa oleifera and/or Cichorium intybus addition to broiler chicken ration. Journal of Veterinary Medicine and Animal Health 9(10): 255–63. DOI: https://doi.org/10.5897/JVMAH2017.0611
Pushparaj P N, Low H K, Manikandan J, Tan B H K and Tan C H. 2007. Antidiabetic effects of Cichorium intybus
in streptozotocin induced diabetic rats. Journal of Ethnopharmocology 111(2): 430–34.
Saeed M, Baloch A R, Wang M, Soomro R N, Baloch A M, Bux B A, Arian M A, Faraz S S and Zakriya H M. 2015.
Use of Cichorium intybus leaf extract as growth promoter, hepatoprotectant and immune modulant in broilers. Journal of Animal Production Advances 5(1): 585–91. DOI: https://doi.org/10.5455/japa.20150118041009
Sarwar M S. 2013. ‘Effect of chicory leaves extract, extracted in water at different pH levels (3,7 and 12) on growth, nutrient digestibility, hematology, immune response and economics of broilers.’ M.Sc. Hons. Thesis, University of Agriculture, Faisalabad, Pakistan. Sharma M, Afaque A, Dwivedi S, Jairajpuri Z S, Shamsi Y, Khan M F, Khan M I and Ahmed D. 2019. Cichorium intybus attenuates streptozotocin induced diabetic cardiomyopathy via inhibition of oxidative stress and inflammatory response in rats. Interdisciplinary Toxicology 12(3): 111–19. DOI: https://doi.org/10.2478/intox-2019-0013
Snedecor G W and Cochran W G. 1967. Statistical Methods. 6th Edition. Iowa State University Press, Ames, lowa.
Street R A, Sidana J and Prinsloo G. 2013. Cichorium intybus: Traditional uses, phytochemistry, pharmacology and
toxicology. Evidence Based Complementary and Alternative Medicine. https://doi.org/10.1155/2013/579319. DOI: https://doi.org/10.1155/2013/579319
Thapa B R and Walia A. 2007. Liver function tests and their interpretation. Indian Journal of Pediatrics 74: 663–71. DOI: https://doi.org/10.1007/s12098-007-0118-7
Zaman R, Alam A, Jafri M, Sofi G and Ahmed G. 2017. Nephroprotective effect of Beekh Kasni (Roots of Cichorium
intybus) in the form of methanolic and aqueous extract in gentamycin induced rat models. Journal of Pharmacognosy and Phytochemistry 6: 337–41.
Zuo Y, Wang C, Zhou J, Sachdeva A and Ruelos V C. 2008. Simultaneous determination of creatinine and uric acid in
human urine by high performance liquid chromatography. Analytical Sciences 24(12): 1589–92.
Downloads
Submitted
Published
Issue
Section
License
The copyright of the articles published in The Indian Journal of Animal Sciences is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.