Myostatin (GDF8) gene and its intriguing role in regulating growth in poultry
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Keywords:
Association, Gene editing, Growth marker, Myostatin, RNAi, SNPsAbstract
Myostatin, also called as growth differentiating factor 8 (GDF8), a negative regulator of growth in chicken has been characterized at nucleotide and protein level. The total length of the coding frame is 1128 bp encoding 375 amino acids pro-myostatin consisting of first 23 amino acids forming the signal peptide, next 243 amino acids as pre-protein and remaining 109 amino acids forming mature peptide. The protein in dimeric form is biologically active in chicken. This gene harbours many SNPs at promoter, 5’-UTR, exons, introns and 3’-UTR across the breeds of chicken in which coding region had higher level of polymorphism than non-coding regions. The SNPs had significant association with many economic traits such as growth traits, carcass traits, and immune response traits. The body weights of poultry can be rapidly enhanced by knocking down the expression of myostatin gene by RNAi and knocking out the gene by gene-editing, and in one generation more than 26% improvement in body weight was achieved. It is concluded that myostatin has been a promising molecule associated with controlling growth in chicken which may be considered to determine genetic markers for growth and other economically important traits for further use in selection programme, and to improve growth traits rapidly by inhibiting its expression through RNAi and gene editing in poultry.
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References
Abdel-Gawad M M, Azab M E, Ismail R S, Nafeaa A A and A El-Magd M E. 2017. The action of myo-pro gene on the morphology of skeletal muscle of Fayoumi chicken. Benha Veterinary Medical Journal 33(2): 129–36. DOI: https://doi.org/10.21608/bvmj.2017.30013
Abdel-Gawad M M, Azaba M E, Ismaila R S, Nafeaa A A and El- Magd M E A. 2019. Effect of myostatin inhibitor (myostatin-propeptide) on expression of myogenic genes in chick embryo. Arabian Journal of Medical Sciences 2(2): 30–34.
Allen D L and Unterman T G. 2007. Regulation of myostatin expression and myoblast differentiation by FoxO and SMAD transcription factors. American Journal of Physiology-Cell Physiology 292(1): 188–99. DOI: https://doi.org/10.1152/ajpcell.00542.2005
Amthor H, Huang R, McKinnell I, Christ B, Kambadur R, Sharma M and Patel K. 2002. The regulation and action of myostatin as a negative regulator of muscle development during avian embryogenesis. Developmental Biology 251(2): 241–57. DOI: https://doi.org/10.1006/dbio.2002.0812
Andermatt I and Stoeckli E T. 2014. RNAi-based gene silencing in chicken brain development. Methods in Molecular Biology 1082: 253–66. DOI: https://doi.org/10.1007/978-1-62703-655-9_17
Baron E E, Wenceslau A A, Alvares L E, Nones K, Ruy D C, Schmidt G S and Ledur M C. 2002. High level of polymorphism in the myostatin chicken gene. Proc. 7th World Congress on Genetics Applied to Livestock Production, Montpellier, France.
Bhattacharya T K. 2017. Development of an improved coloured broiler chicken line through MAS. ICAR-DPR News 17(2): 2.
Bhattacharya T K and Chatterjee R N. 2013. Polymorphism of the myostatin gene and its association with growth traits in chicken. Poultry Science 92(4): 910–15. DOI: https://doi.org/10.3382/ps.2012-02736
Bhattacharya T K and Chatterjee R N. 2014. ‘Improvement in meat production with RNAi in poultry.’ ICAR News 20(4): 7.
Bhattacharya T K, Chatterjee R N, Dushyanth K, Paswan C, Shukla R and Shanmugam M. 2015a. Polymorphism and expression of insulin-like growth factor 1 (IGF1) gene and its association with growth traits in chicken. British Poultry Science 56(4): 398–407. DOI: https://doi.org/10.1080/00071668.2015.1041098
Bhattacharya T K, Chatterjee R N, Dushyanth K and Shukla R. 2015. Cloning, characterization and expression of myostatin (growth differentiating factor-8) gene in broiler and layer chicken (Gallus gallus). Molecular Biology Reports 42(2): 319–27. DOI: https://doi.org/10.1007/s11033-014-3753-x
Bhattacharya T K, Chatterjee R N, Sharma R P, Niranjan M and Rajkumar U. 2011. Associations between novel polymorphisms at the 5′-UTR region of the prolactin gene and egg production and quality in chickens. Theriogenology 75(4): 655–61. DOI: https://doi.org/10.1016/j.theriogenology.2010.10.005
Bhattacharya T K, Chatterjee R N, Sharma R P, Raj Kumar U and Niranjan M. 2011. Genetic polymorphism of exon 1 of myostatin gene in a broiler and a layer chicken line. Indian Veterinary Journal 88(5): 34–36.
Bhattacharya T K, Chatterjee R N, Sharma R P, Rajkumar U, Niranjan M and Reddy B L N. 2011. Exon-2 of Myostatin gene in chicken. Indian Veterinary Journal 88: 39–40.
Bhattacharya T K, Paswan C, Chatterjee R N, Bhanja S K and Dange M. 2018. Development of an improved coloured broiler chicken line through MAS. ICAR-DPR News 18(1): 3.
Bhattacharya T K, Shukla R, Chatterjee R N and Bhanja S K. 2019. Comparative analysis of silencing expression of myostatin (MSTN) and its two receptors (ACVR2A and ACVR2B) genes affecting growth traits in knock down chicken. Scientific Reports 9(1):1-13. DOI: https://doi.org/10.1038/s41598-019-44217-z
Bhattacharya T K, Shukla R, Chatterjee R N and Dushyanth K. 2017. Knock down of the myostatin gene by RNA interference increased body weight in chicken. Journal of Biotechnology 241: 61–68. DOI: https://doi.org/10.1016/j.jbiotec.2016.11.012
Boschiero C, Gheyas A, Godoy T, Moreira G, Grade C V C, Gasparin G and Coutinho L. 2013. SNPS and INDELS detection in the chicken myostatin gene and its intergenic regions. 5th Symposium on Animal Functional Genomics- ISAFG 2013.
Chen P R, Suh Y, Shin S, Woodfint R M, Hwang S and Lee K. 2019. Exogenous expression of an alternative splicing variant of myostatin prompts leg muscle fiber hyperplasia in Japanese quail. International Journal of Molecular Sciences 20(18): 4617. DOI: https://doi.org/10.3390/ijms20184617
Crisa A, Marchitelli C, Savarese M C and Valentini A. 2003. Sequence analysis of myostatin promoter in cattle. Cytogenetic and Genome Research 102(1-4): 48–52. DOI: https://doi.org/10.1159/000075724
Dementeva N V, Mitrofanova O V, Tyshchenko V I, Terletskiy V P and Yakovlev A F. 2017. The rate of weight gain and productivity of a chicken broiler cross with various polymorphic types of the myostatin gene. Russian Journal of Genetics: Applied Research 7(1): 1–5. DOI: https://doi.org/10.1134/S207905971701004X
Divya D, Bhattacharya T K, Gnana Prakash M, Chatterjee R N, Shukla R, Vishnu G and Dushyanth K. 2018. Molecular characterization and expression profiling of BMP 3 gene in broiler and layer chicken. Molecular Biology Reports 45(4): 477–95. DOI: https://doi.org/10.1007/s11033-018-4184-x
Dou T, Li Z, Wang K, Liu L, Rong H, Xu Z and Jia J. 2018. Regulation of myostatin expression is associated with growth and muscle development in commercial broiler and DMC muscle. Molecular Biology Reports 45(4): 511–22. DOI: https://doi.org/10.1007/s11033-018-4187-7
Dushyanth K, Bhattacharya T K, Paswan C, Sitaramamma T, Shukla R and Chatterjee R N. 2016a. Structural variation in Fstn Exon 3 gene and its association with growth traits in chicken. Journal of Animal Research 6: 471–74. DOI: https://doi.org/10.5958/2277-940X.2016.00048.6
Dushyanth K, Bhattacharya T K, Paswan C, Shukla R, Vishnu P G and Chatterjee R N. 2016b. Characterization of exon4 of FSTN gene and its association with growth traits in PD-1 broiler chicken. Journal of Animal Research 6(5): 967–69. DOI: https://doi.org/10.5958/2277-940X.2016.00126.1
Dushyanth K, Bhattacharya T K, Shukla R, Chatterjee R N, Sitaramamma T, Paswan C and Guru Vishnu P. 2016c. Gene expression and polymorphism of Myostatin gene and its association with growth traits in chicken. Animal Biotechnology 27(4): 269–77. DOI: https://doi.org/10.1080/10495398.2016.1182541
Dybus A, Proskura W S, Sadkowski S and Pawlina E. 2013. A single nucleotide polymorphism in exon 3 of the myostatin gene in different breeds of domestic pigeon (Columba livia var. domestica). Veterinarni Medicina 58(1): 32–38. DOI: https://doi.org/10.17221/6654-VETMED
Gonzalez-Cadavid N F, Taylor W E, Yarasheski K, Sinha-Hikim I, Ma K, Ezzat S and Bhasin S. 1998. Organization of the human myostatin gene and expression in healthy men and HIV-infected men with muscle wasting. Proceedings of the National Academy of Sciences 95(25): 14938–43. DOI: https://doi.org/10.1073/pnas.95.25.14938
Gu Z L, Zhang H F, Zhu D H and Li H. 2002. Single nucleotide polymorphism analysis of the chicken Myostatin gene in different chicken lines. Acta Genetica Sinica 29(7): 599–606.
Gu Z, Zhu D, Li N, Li H, Deng X and Wu C. 2004. The single nucleotide polymorphisms of the chicken myostatin gene are associated with skeletal muscle and adipose growth. Science in China Series C: Life Sciences 47(1): 25–30.
Guru Vishnu P B, Bhattacharya T K, Kumar P, Chaterjee R N, Ravi Kumar G V P P S, Paswan C and Rajendra Prasad A. 2017. Expression profiling of activin type IIB receptor during ontogeny in broiler and indigenous chicken. Animal Biotechnology 28(1): 26–36. DOI: https://doi.org/10.1080/10495398.2016.1194287
Heintzman N D and Ren B. 2007. The gateway to transcription: identifying, characterizing and understanding promoters in the eukaryotic genome. Cellular and Molecular Life Sciences 64(4): 386–400. DOI: https://doi.org/10.1007/s00018-006-6295-0
Hill E W, McGivney B A, Gu J, Whiston R and MacHugh D E. 2010. A genome-wide SNP-association study confirms a sequence variant (g. 66493737C> T) in the equine myostatin (MSTN) gene as the most powerful predictor of optimum racing distance for Thoroughbred racehorses. BMC Genomics 11(1): 1–10. DOI: https://doi.org/10.1186/1471-2164-11-552
Hu W, Chen S, Zhang R and Lin Y. 2013. Single nucleotide polymorphisms in the upstream regulatory region alter the expression of myostatin. In Vitro Cellular and Developmental Biology-Animal 49(6): 417–23. DOI: https://doi.org/10.1007/s11626-013-9621-5
Ivey F M, Roth S M, Ferrell R E, Tracy B L, Lemmer J T, Hurlbut D E and Hurley B F. 2000. Effects of age, gender, and myostatin genotype on the hypertrophic response to heavy resistance strength training. Journals of Gerontology Series A: Biological Sciences and Medical Sciences 55(11): 641–48. DOI: https://doi.org/10.1093/gerona/55.11.M641
Jain H, Singh S, Kadam M and Sarkhel B C. 2010. Knockdown of the myostatin gene by RNA interference in caprine fibroblast cells. Journal of Biotechnology 145(2): 99–102. DOI: https://doi.org/10.1016/j.jbiotec.2009.10.017
Jeanplong F, Sharma M, Somers W G, Bass J J and Kambadur R. 2001. Genomic organization and neonatal expression of the bovine myostatin gene. Molecular and Cellular Biochemistry 220(1): 31–37. DOI: https://doi.org/10.1023/A:1010801511963
Kanjanaworakul P, Sawatdichaikul O and Poompuang S. 2016. cDNA sequence and protein bioinformatics analyses of MSTN in African catfish (Clarias gariepinus). Molecular Biology Reports 43(4): 283–93. DOI: https://doi.org/10.1007/s11033-016-3961-7
Khaerunnisa I, Pramujo M and Arief I. 2016. Polymorphism of the T4842G myostatin gene is associated with carcass characteristics in Indonesian chickens. International Journal of Poultry Science 15(8): 316–24. carcass characteristics in Indonesian chickens. DOI: https://doi.org/10.3923/ijps.2016.316.324
Kim G D, Lee J H, Song S, Kim S W, Han J S, Shin S P and Park S. 2020. Generation of myostatin-knockout chickens mediated by D10A-Cas9 nickase. FASEB Journal 34(4): 5688–96. DOI: https://doi.org/10.1096/fj.201903035R
Kim S, Park K, Shin C, Cho N H, Ko J J, Koh I and Kwack K. 2013. Diplotyper: diplotype-based association analysis. BMC Medical Genomics 6(2): 1–8. DOI: https://doi.org/10.1186/1755-8794-6-S2-S5
Kocamis H, Kirkpatrick-Keller D C, Richter J and Killefer J. 1999. The ontogeny of myostatin, follistatin and activin-B mRNA expression during chicken embryonic development. Growth, Development, and Aging: GDA 63(4): 143–50.
Kubota K, Sato F, Aramaki S, Soh T, Yamauchi N and Hattori M A. 2007. Ubiquitous expression of myostatin in chicken embryonic tissues: its high expression in testis and ovary. Comparative Biochemistry and Physiology Part A: Molecular and Integrative Physiology 148(3): 550–55. DOI: https://doi.org/10.1016/j.cbpa.2007.07.004
Kumar R, Singh S P, Kumari P and Mitra A. 2014. Small interfering RNA (siRNA)-mediated knockdown of myostatin influences the expression of myogenic regulatory factors in caprine foetal myoblasts. Applied Biochemistry and Biotechnology 172(3): 1714–24. DOI: https://doi.org/10.1007/s12010-013-0582-7
Kumar S B, Dilbaghi N, Ahlawat S P S, Mishra B, Tantia M S and Vijh R K. 2007. Genetic relationship among chicken populations of India based on SNP markers of myostatin gene (GDF 8). International Journal of Poultry Science 6(9): 684–88. DOI: https://doi.org/10.3923/ijps.2007.684.688
Lee J, Kim D H and Lee K. 2020. Muscle hyperplasia in Japanese quail by single amino acid deletion in MSTN propeptide. International Journal of Molecular Sciences 21(4): 1504. DOI: https://doi.org/10.3390/ijms21041504
Lee J H, Kim S W and Park T S. 2017. Myostatin gene knockout mediated by Cas9-D10A nickase in chicken DF1 cells without off-target effect. Asian Australasian Journal of Animal Sciences 30(5): 743. DOI: https://doi.org/10.5713/ajas.16.0695
Lee S J. 2010. Extracellular regulation of myostatin: a molecular rheostat for muscle mass. Immunology, Endocrine and Metabolic Agents in Medicinal Chemistry (Formerly Current Medicinal Chemistry-Immunology, Endocrine and Metabolic Agents) 10(4): 183–94. DOI: https://doi.org/10.2174/187152210793663748
McCroskery S, Thomas M, Maxwell L, Sharma M and Kambadur R. 2003. Myostatin negatively regulates satellite cell activation and self-renewal. Journal of Cell Biology 162: 1135–47. DOI: https://doi.org/10.1083/jcb.200207056
McFarland D C, Velleman S G, Pesall J E and Liu C. 2007. The role of myostatin in chicken (Gallus domesticus) myogenic satellite cell proliferation and differentiation. General and Comparative Endocrinology 151(3): 351–57. DOI: https://doi.org/10.1016/j.ygcen.2007.02.006
McPherron A C, Lawler A M and Lee S J. 1997. Regulation of skeletal muscle mass in mice by a new TGF-p superfamily member. Nature 387(6628): 83–90. DOI: https://doi.org/10.1038/387083a0
Mota L S, Curi R A, Palmieri D A, Borges A S, Lopes C R, Barbosa J D and Gimenes M A. 2006. Sequence characterization of coding regions of the myostatin gene (GDF8) from Brazilian Murrah buffaloes (Bubalus bubalis) and comparison with the Bos taurus sequence. Genetics and Molecular Biology 29: 79–82. DOI: https://doi.org/10.1590/S1415-47572006000100016
Paswan C, Bhattacharya T K, Nagaraj C S, Chaterjee R N and Jayashankar M R. 2014. SNPs in minimal promoter of myostatin (GDF-8) gene and its association with body weight in broiler chicken. Journal of Applied Animal Research 42(3): 304–09. DOI: https://doi.org/10.1080/09712119.2013.846859
Petersen J L, Mickelson J R, Rendahl A K, Valberg S J, Andersson L S, Axelsson J and McCue M E. 2013. Genome-wide analysis reveals selection for important traits in domestic horse breeds. PLoS Genetics 9(1): e1003211. DOI: https://doi.org/10.1371/journal.pgen.1003211
Rao M, Baraban J H, Rajaii F and Sockanathan S. 2004. In vivo comparative study of RNAi methodologies by in ovo electroporation in the chick embryo. Developmental dynamics: an official publication of the American Association of Anatomists 231(3): 592–600. DOI: https://doi.org/10.1002/dvdy.20161
Reardon K A, Davis J, Kapsa R M, Choong P and Byrne E. 2001. Myostatin, insulin-like growth factor-1 and leukemia inhibitory factor mRNAs are upregulated in chronic human disuse muscle atrophy. Muscle and Nerve: Official Journal of the American Association of Electrodiagnostic Medicine 24(7): 893–99. DOI: https://doi.org/10.1002/mus.1086
Rı́os R, Carneiro I, Arce V M and Devesa J. 2002. Myostatin is an inhibitor of myogenic differentiation. American Journal of Physiology-Cell Physiology 282(5): C993-99. DOI: https://doi.org/10.1152/ajpcell.00372.2001
Salerno M S, Thomas M, Forbes D, Watson T, Kambadur R and Sharma M. 2004. Molecular analysis of fiber type-specific expression of murine myostatin promoter. American Journal of Physiology-Cell Physiology 287(4): C1031-40. DOI: https://doi.org/10.1152/ajpcell.00492.2003
Satheesh P, Bhattacharya T K, Kumar P, Chatterjee R N, Dhara S K, Paswan C and Dushyanth K. 2016. Gene expression and silencing of activin receptor type 2A (ACVR2A) in myoblast cells of chicken. British Poultry Science 57(6): 763–70. DOI: https://doi.org/10.1080/00071668.2016.1219693
Sato F, Kurokawa M, Yamauchi N and Hattori M A. 2006. Gene silencing of myostatin in differentiation of chicken embryonic myoblasts by small interfering RNA. American Journal of Physiology-Cell Physiology 291(3): 538–45. DOI: https://doi.org/10.1152/ajpcell.00543.2005
Scheuermann G N, Bilgili S F, Tuzun S and Mulvaney D R. 2004. Comparison of chicken genotypes: myofiber number in pectoralis muscle and myostatin ontogeny. Poultry Science 83(8): 1404–12. DOI: https://doi.org/10.1093/ps/83.8.1404
Schuelke M, Wagner K R, Stolz L E, Hübner C, Riebel T, Kömen W and Lee S J. 2004. Myostatin mutation associated with gross muscle hypertrophy in a child. New England Journal of Medicine 350(26): 2682–88. DOI: https://doi.org/10.1056/NEJMoa040933
Sharma M, Langley B, Bass J and Kambadur R. 2001. Myostatin in muscle growth and repair. Exercise and Sport Sciences Reviews 29(4): 155-58. DOI: https://doi.org/10.1097/00003677-200110000-00004
Song X C, Xu C, Yue Z G, Wang L, Wang G W and Yang F H. 2016. Bioinformatic analysis based on the complete coding region of the MSTN gene within and among different species. Genetics and Molecular Research 15(10.4238). DOI: https://doi.org/10.4238/gmr.15025031
Spiller M P, Kambadur R, Jeanplong F, Thomas M, Martyn J K, Bass J J and Sharma M. 2002. The myostatin gene is a downstream target gene of basic helix-loop-helix transcription factor MyoD. Molecular and Cellular Biology 22(20): 7066– 82. DOI: https://doi.org/10.1128/MCB.22.20.7066-7082.2002
Starck C S and Sutherland-Smith A J. 2011. The C313Y Piedmontese mutation decreases myostatin covalent dimerisation and stability. BMC Research Notes 4(1): 1–12. DOI: https://doi.org/10.1186/1756-0500-4-442
Tao Z, Zhu C, Song C, Song W, Ji G, Shan Y and Li H. 2015. Lentivirus-mediated RNA interference of myostatin gene affects MyoD and Myf5 gene expression in duck embryonic myoblasts. British Poultry Science 56(5): 551–58. DOI: https://doi.org/10.1080/00071668.2015.1085958
Tries R S, Chen T, Da Vies M V, Tomkinson K N, Pearson A A, Shakey Q A and Wolfman N M. 2001. GDF-8 propeptide binds to GDF-8 and antagonizes biological activity by inhibiting GDF-8 receptor binding. Growth Factors 18(4): 251–59. DOI: https://doi.org/10.3109/08977190109029114
Thomas M, Langley B, Berry C, Sharma M, Kirk S, Bass J and Kambadur R. 2000. Myostatin, a negative regulator of muscle growth, functions by inhibiting myoblast proliferation. Journal of Biological Chemistry 275(51): 40235–43. DOI: https://doi.org/10.1074/jbc.M004356200
Tripathi A K, Aparnathi M K, Vyavahare S S, Ramani U V, Rank D N and Joshi C G. 2012. Myostatin gene silencing by RNA interference in chicken embryo fibroblast cells. Journal of Biotechnology 160(3-4): 140–45. DOI: https://doi.org/10.1016/j.jbiotec.2012.03.001
Vishnu P G, Bhattacharya T K, Kumar P, Chatterjee R N, Paswan C and Dushyanth K. 2016. Single nucleotide polymorphism in the 5′-upstream region of activin A receptor, type IIB in Aseel Chicken. Journal of Animal Research 6(2): 221–25. DOI: https://doi.org/10.5958/2277-940X.2015.00164.3
Wang Y, Xu H Y, Gilbert E R, Peng X, Zhao X L, Liu Y P and Zhu Q. 2014. Detection of SNPs in the TBC1D1 gene and their association with carcass traits in chicken. Gene 547(2): 288–94. DOI: https://doi.org/10.1016/j.gene.2014.06.061
Wilt F H and Hake S. 2004. Principles of Developmental Biology. Inc. ISBN 0-393-97430-8: pp450.
Wolfma N M, McPherron A C, Pappano W N, Davies M V, Song K, Tomkinson K N and Lee S J. 2003. Activation of latent myostatin by the BMP-1/tolloid family of metalloproteinases. Proceedings of the National Academy of Sciences 100(26): 15842–46. DOI: https://doi.org/10.1073/pnas.2534946100
Xue L, Qian K, Qian H, Li L, Yang Q and Li M. 2006. Molecular cloning and characterization of the myostatin gene in croceine croaker, Pseudosciaena crocea. Molecular Biology Reports 33(2): 129–35. DOI: https://doi.org/10.1007/s11033-006-0015-6
Ye X, Brown S R, Nones K, Coutinho L L, Dekkers J C and Lamont S J. 2007. Associations of myostatin gene polymorphisms with performance and mortality traits in broiler chickens. Genetics Selection Evolution 39(1): 73–89. DOI: https://doi.org/10.1186/1297-9686-39-1-73
Zammit P S. 2017. Function of the myogenic regulatory factors Myf5, MyoD, Myogenin and MRF4 in skeletal muscle, satellite cells and regenerative myogenesis. In Seminars in Cell and Developmental Biology 72: 19–32. DOI: https://doi.org/10.1016/j.semcdb.2017.11.011
Zandi S, Zamani P and Mardani K. 2013. Myostatin gene polymorphism and its association with production traits in western azerbaijan native chickens. Iranian Journal of Applied Animal Science 3: 611–15.
Zhang G X, Zhao X H, Wang J Y, Ding F X and Zhang L. 2012. Effect of an exon 1 mutation in the myostatin gene on the growth traits of the Bian chicken. Animal Genetics 43(4): 458–59. DOI: https://doi.org/10.1111/j.1365-2052.2011.02274.x
Zhang X X, Ran J S, Lian T, Li Z Q, Yang C W, Jiang X S and Liu Y P. 2019. The single nucleotide polymorphisms of myostatin gene and their associations with growth and carcass traits in Daheng broiler. Brazilian Journal of Poultry Science 21(3). DOI: https://doi.org/10.1590/1806-9061-2018-0808
Zhiliang G U, Zhu D, Li N, Li H, Deng X and Wu C. 2004. The single nucleotide polymorphisms of the chicken myostatin gene are associated with skeletal muscle and adipose growth. Science in China Series C: Life Sciences 47(1): 25–30. DOI: https://doi.org/10.1360/02yc0201
Zhu J, Li Y, Shen W, Qiao C, Ambrosio F, Lavasani M and Huard J. 2007. Relationships between transforming growth factor-β1, myostatin, and decorin: implications for skeletal muscle fibrosis. Journal of Biological Chemistry 282(35): 25852–63. DOI: https://doi.org/10.1074/jbc.M704146200
Zimmers T A, Davies M V, Koniaris L G, Haynes P, Esquela A F, Tomkinson K N and Lee S J. 2002. Induction of cachexia in mice by systemically administered myostatin. Science 296(5572): 1486–88. DOI: https://doi.org/10.1126/science.1069525
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