Preliminary studies on selection of a vaccine candidate of Classical Swine Fever Virus

A RAMESH; K KUMANAN

doi:10.56093/ijans.v93i10.125817

Authors

A RAMESH Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu 600 051 India
K KUMANAN Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu

https://doi.org/10.56093/ijans.v93i10.125817

Keywords:

Classical swine fever, PK-15, Vaccine candidate

Abstract

Classical swine fever is an economically important and endemic disease of swine in India. Though vaccination is the best way of controlling the disease, currently available vaccines are not very effective. Cell culture adapted local strains of viral pathogens could be a better choice for large scale vaccine production and better immunity. Two local isolates and one lapinized strain of Classical swine fever virus were adapted to PK-15 cells and assessed for their suitability as a vaccine candidate. The CSFV isolate, CSFV-Ind-TN/Veng-09-020–Vn, with a TCID₅₀ titre of 9.5 after 40 continuous passages was found to give better seroconversion, good protection against virulent CSFV challenge and could be a better candidate vaccine strain.

Downloads

Download data is not yet available.

References

Chander V, Nandi S, Ravishankar C and Upmanyu V. 2014. Classical swine fever in pigs: recent developments and future perspectives. Animal Health Research Reviews 15: 87–101. DOI: https://doi.org/10.1017/S1466252314000024

Gonzalez S M, Simo M P, Munoz M, Bohorquez J A, Rosell R, Summerfield A, Domingo M, Ruggli N and Ganges L. 2015. Efficacy of a live attenuated vaccine in classical swine fever virus postnatally persistently infected pigs, Veterinary Research.

Kumar H, Mahajan V, Sharma S,Alka, Singh R,Arora K, Banga H S, Verma S, Kaur P, Meenakshi K and Sandhu K S. 2007. Concurrent pasteurellosis and classical swine fever in Indian pig. Journal of Swine Health and Production 15: 279–83.

Manoharan S, Vadivoo V S, Aishwarya Devi J, Sri Latha K, Kumanan K and Sai Kumar G. 2011. Outbreak of Classical swine fever in Andhra Pradesh. Indian Veterinary Journal 88: 13–15.

Rathnapraba S, Vadivoo V S, Manoharan S, Logesh K and Kumanan K. 2012. Molecular detection of classical swine fever from a field outbreak in Tamil Nadu. Indian Journal of Veterinary Pathology 36: 22–27.

Rathnapraba S, Kumanan K, Vijayarani K, Palanisammi M and Gunaseelan L. 2014. Molecular typing of classical swine fever viruses from field outbreaks in Southern India. Indian Journal of Animal Sciences 84: 723–27.

Reed L J and Muench H. 1938. A simple method of estimating fifty per cent endpoints. The American Journal of Hygiene 27: 493–97. DOI: https://doi.org/10.1093/oxfordjournals.aje.a118408

Rout M and Saikumar G. 2012. Pathology of classical swine fever in slaughtered pigs. Indian Journal of Veterinary Pathology 36: 136–42.