Comparative evaluation of pinhole and section ligation release castration techniques in male dogs
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Keywords:
Castration, Dog, Pinhole, Section ligation releaseAbstract
The present study was carried out to compare two minimally invasive surgical in situ castration techniques in dogs. Male dogs (n-12) brought to the department for elective castration were randomly divided into two groups comprising six in each group. In Group I, pinhole (PH) castration, and section ligation release (SLR) castration in group II were performed. Physiological parameters, testicular dimensions, haemato-biochemical analysis, ultrasonographic examination and pain scores were recorded on 0th, 3rd, 14th and 28th day. Testicular tissue biopsy sample for histopathological examination were taken on 0th and 28th day. Physiological parameters, testicular breadth and height revealed non-significant variation, while significant difference was observed in testicular circumference and volume in both the groups. Non-significant difference was noted in haemoglobin, packed cell volume, total leucocyte count and differential leucocyte count within or in between the groups. Cortisol and MDA were significantly higher on 3rd day, whereas GSH was significantly lower on 3rd day in comparison to other time intervals in both the groups. Pain scores were significantly higher on 3rd day in both the groups and were also significantly more in group II. Testosterone levels were markedly reduced in both the techniques, but values were significantly lesser in group II as compared to group I. Ultrasonographic and histopathological examination revealed marked testicular degeneration in both the techniques. It was concluded that pinhole castration technique is minimally invasive, less time consuming, economical and has less post-operative complications in comparison to section ligation and release technique.
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Abou-Ahmed H M, Mahmoud H, Kammar E L, Mahmoud S, EL-Neweshy, Ramadan E Abdel-Wahed. 2012. Comparative evaluation of three in situ castration techniques for sterilizing Donkeys: Incision ligation (a novel technique), section ligation release, and pinhole. Journal of Equine Veterinary Science 32: 711–18. DOI: https://doi.org/10.1016/j.jevs.2012.02.016
Ahmad N and Noakes D E. 1995. Ultrasound imaging in determining the presence of testicular degeneration in two male goats. British Veterinary Journal 151: 101–10. DOI: https://doi.org/10.1016/S0007-1935(05)80069-7
Ahmad N, Noakes D E and Subandrio A L. 1991. B mode real time ultrasonographic imaging of testis and epididymis of sheep and goats. Veterinary Research 4: 491–96. DOI: https://doi.org/10.1136/vr.128.21.491
Al-Asadi R and Al-Kadi K K. 2012. Non-surgical castration in bucks a comparative study between chemical castration and external ligation of the spermatic cord. AL-Qadisiyah Journal of Veterinary Medicine Sciences 11(1): 92–102. DOI: https://doi.org/10.29079/vol11iss1art176
Arteaga A A, Albert D B and Leonardo F C B. 2005. Relationship between semen quality and pixel–intensity of testicular ultrasonograms after scrotal insulation in beef bulls. Theriogenology 64: 408–15. DOI: https://doi.org/10.1016/j.theriogenology.2004.12.008
Baba M A, Fazili M R, Athar H, Mir M S, Moulvi B A and Khan I. 2012. Comparison of analgesic effect of meloxicam with ketoprofen in male stray dogs undergoing pinhole castration. Asian Journal of Animal Sciences 6(4): 164–73. DOI: https://doi.org/10.3923/ajas.2012.164.173
Baba M A, Fazili M R, Athar H, Mir M S, Moulvi B A and Khan I. 2013. Pinhole castration technique: An alternative to orchiectomy in stray dogs. Animal Reproduction Science 137(2): 113–18. DOI: https://doi.org/10.1016/j.anireprosci.2012.11.015
Bergh A, Collin O and Lisboan E. 2001. Effects of cute graded reductions in testicular blood flow on testicular morphology in the adult rat. Biology of Reproduction 64: 13–20. DOI: https://doi.org/10.1095/biolreprod64.1.13
Beutler E, Duron O and Kelly M K. 1963. Improved method for the determination of blood glutathione. Journal of Laboratory and Clinical Medicine 61(5): 882–88.
Bhagyalakshmi J, Balasundaram K, Selvaraj P, Kathirvel S, Balachandran P and Chandana G S S. 2020. Gross, histology and histochemical analysis of the testis in dog (Canis lupus familiaris). Journal of Entomology and Zoology Studies 8(4): 69–73.
Camkerten I, Sahin T, Borazan G, Gokcen, A, Erel O and Das A. 2009. Evaluation of blood oxidant/antioxidant balance in dogs with sarcoptic mange. Veterinary Parasitology 161: 106–09. DOI: https://doi.org/10.1016/j.vetpar.2008.12.019
Cohen R D H, King B D, Thomas L R and Janzen E D. 1990. Efficacy and stress of chemical versus surgical castration of cattle. Canadian Journal for Animal Science 70: 1063–72. DOI: https://doi.org/10.4141/cjas90-129
Fazili M R, Bhattacharyya H K, Buchoo B A, Kirmani M A, Dirazi M M and Khan I. 2009. Evaluation of pinhole castration technique in rams. Small Ruminant Research 84: 61–64. DOI: https://doi.org/10.1016/j.smallrumres.2009.06.004
Hellyer P, Rodan I, Brunt J, Downing R, Hagedorn J E and Robertson S A. 2007. Pain management guidelines for Dogs and Cats. Journal of American Animal Hospital Association 43: 235–48. DOI: https://doi.org/10.5326/0430235
Kehlet H B. 1991. Neurohumoral response to surgery and pain in man. Proceedings of 6th World Congress on Pain, Elsevier, The Netherlands, Amsterdam, pp. 35-40.
Khade S K. 2006. ‘Comparative evaluation of castration by two different surgical methods in dogs.’ M.V.Sc. thesis, Maharashtra Animal and Fisheries Sciences University, Nagpur.
Kutzler M and Wood A. 2006. Non-surgical methods of contraception and sterilization. Theriogenology 66(3): 514–25. DOI: https://doi.org/10.1016/j.theriogenology.2006.04.014
Mahalingam A, Kumar N, Maiti S K, Sharma A K, Dimri U and Kataria M. 2009. Laparoscopic sterilization vs Open method sterilization in dogs: A comparison of two techniques. Turkish Journal of Veterinary and Animal Sciences 33(5): 427–36. DOI: https://doi.org/10.3906/vet-0805-30
Mahalingam A, Kumar N, Maiti S K, Sharma A K, Dimri U and Kataria M. 2014. Laparoscopic vasectomy vs Laparoscopic sterilization in dogs: A comparison of two techniques. World Journal of Laparoscopic Surgery 7(1): 7–15. DOI: https://doi.org/10.5005/jp-journals-10007-1210
Misk N and Seleim M. 1987. An improved technique for castrating equids. Veterinary Record 115: 372–75.
Munahi K and AbidTh A. 2011. Castration of Iraqi local bucks by bilateral spermatic cord torsion compared with double ligation of spermatic cord. Al-Qadisiyah Journal of Veterinary Medicine and Sciences 10(2): 21–35. DOI: https://doi.org/10.29079/vol10iss2art151
Okwee-Acai J, Acon J, Okello-Owiny D, Agwai B and Oloya J. 2008. Evaluation of pinhole castration as an alternative technique for goat sterilization. Bulletin of Animal Health and Production in Africa 56: 299–306. DOI: https://doi.org/10.4314/bahpa.v56i4.43301
Okwee-Acai J, Akunu B, Agwai B, Ekakoro E, Sajjakambwe P and Acon J. 2012. An evaluation of stress responses, simplicity and cost of pinhole castration as an alternative technique for male dog sterilization. Research Opinions in Animal and Veterinary Sciences 2(1): 55–59.
Placer Z A, Cushman L and Johnson B. 1966. Estimation of product of lipid peroxidation (Malondialdehyde) in biochemical system. Analytical Biochemistry 16: 3359–64. DOI: https://doi.org/10.1016/0003-2697(66)90167-9
Ponvijay K S. 2007. Pinhole Castration: A novel minimally invasive technique for in situ spermatic cord ligation. Veterinary Surgery 36: 74–79. DOI: https://doi.org/10.1111/j.1532-950X.2007.00238.x
Pugh C R, Kode L J and Park R D. 1990. Testicular ultrasound in the normal dog. Veterinary Radiology 31(4): 195–99. DOI: https://doi.org/10.1111/j.1740-8261.1990.tb01810.x
Rinaldi L, Biggeri A, Carbone S and Musella V. 2006. Canine faecal contamination and parasitic risk in city of Naples. BMC Veterinary Research 2(1): 29–30. DOI: https://doi.org/10.1186/1746-6148-2-29
Sachin C. 2015. ‘Comparison of conventional and laparoscopic method of cryptorchidectomy in dogs.’ M.V.Sc thesis, Karnataka Veterinary, Animal and Fisheries Sciences University, Bidar.
Saifzadeh S, Hobbenaghi R, Asri-Rezaei S, Shokouhi S J, Naghadeh B and Rohi S M. 2008. Evaluation of section ligation release (SLR) technique devised for castration in the stallion. Reproduction in Domestic Animals 43: 678–84. DOI: https://doi.org/10.1111/j.1439-0531.2007.00969.x
Sakundech K, Chayanon C, Pongsatorn T, Thongchai B and Aengwanich W. 2020. The influence of duration on pain stress, oxidative stress, and total antioxidant power status in female dogs undergoing ovariohysterectomy. Veterinary World 13(1): 160–64. DOI: https://doi.org/10.14202/vetworld.2020.160-164
Sawhney P. 2016. ‘Burdizzo versus pinhole castration in bucks and cattle calves.’ M.V.Sc. thesis, Nanaji Deshmukh Veterinary Science University, Jabalpur.
Sobhy, A, Kassem M M, Abu-Ahmed H and El-khamary A. 2016. Sterilization of male dogs by bilateral electrocoagulation of testicular blood vessels. Alexandria Journal of Veterinary Sciences 8(1): 87–92. DOI: https://doi.org/10.5455/ajvs.207606
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