Evaluation of ionophore resistance in field isolates of Eimeria tenella from Jammu and Kashmir
Abstract views: 59 / PDF downloads: 109 / PDF downloads: 9
Keywords:Anticoccidial, Eimeria tenella, India, Ionophores, Resistance
The present study was conducted to evaluate resistance against ionophores (salinomycin and maduramicin) in four field isolates (F) of Eimeria tenella collected from four distantly located poultry farms from Union territory of Jammu and Kashmir. The purity of isolates was ascertained by COCCIMORPH and ITS-1 gene amplification for E. tenella. For the drug sensitivity trial, Cobb strain, day-old male broiler chicks were used. At the age of 10 days, the birds were transferred ad random in 13 groups to battery cage system. All birds, except non infected non-medicated control were infected with 105 sporulated oocysts each at two weeks. Prophylactic medication with ionophores commenced two days prior to infection in eight groups (two medicated groups for each of the four isolates). Another four groups served as infected and non-medicated control. Seven days post infection, all the surviving birds were sacrificed to study drug resistance by assessing the global index for individual ionophore compounds. The F-1 exhibited resistance against salinomycin and maduramicin whereas, the F-2 showed comparatively improved global index for both salinomycin and maduramicin thus indicating limited efficacy. The F-3 exhibited partial resistance against salinomycin and limited efficacy in case of maduaramicin, and the F-4 was observed to be resistant against salinomycin whereas partially resistant against maduramicin. The present study provides evidence of resistance against maduramicin and salinomycin in field isolates of E. tenella from Union Territory of Jammu and Kashmir which may aid in management of poultry coccidiosis.
Abbas R Z, Iqbal Z, Sindhu Z D, Khan M N and Arshad M. 2008. Identification of cross-resistance and multiple resistance in Eimeria tenella field isolates to commonly used anticoccidials in Pakistan. Journal of Applied Poultry Research 17(3): 361–68. DOI: https://doi.org/10.3382/japr.2008-00027
Attree E, Sanchez-Arsuaga G, Jones M, Xia D, Marugan-Hernandez V, Blake D and Tomley F. 2021. Controlling the causative agents of coccidiosis in domestic chickens; an eye on the past and considerations for the future. CABI Agriculture and Bioscience 2:37. DOI: https://doi.org/10.1186/s43170-021-00056-5
Blake D P, Pastor-Fernández I, Nolan M J and Tomley F M. 2017. Recombinant anticoccidial vaccines - a cup half full? Infection, Genetics and Evolution 55: 358–65. DOI: https://doi.org/10.1016/j.meegid.2017.10.009
Blake D P, Knox J, Dehaeck B, Huntington B, Rathinam T, Ravipati V, Ayoade S, Gilbert W, Adebambo A O, Jatau I D, Raman M, Parker D, Rushton J and Tomley F M. 2020. Re-calculating the cost of coccidiosis in chickens. Veterinary Research 51: 115. DOI: https://doi.org/10.1186/s13567-020-00837-2
Castañón C A B, Fraga J S, Fernandez S, Gruber A and Costa L F. 2007. Biological shape characterization for automatic image recognition and diagnosis of protozoan parasites of the genus Eimeria. Pattern Recognition 40(7):1899–1910. DOI: https://doi.org/10.1016/j.patcog.2006.12.006
Chapman H D. 2014. Milestones in avian coccidiosis research: A review. Poultry Science 93(3): 501–11. DOI: https://doi.org/10.3382/ps.2013-03634
Davies S F M, Joyner L P and Kendall S B. 1963. Coccidiosis.
Oliver and Boyd, Edinburgh and London.
Holdsworth P A, Conway D P, McKenzie M E, Dayton A D, Chapman H D, Mathis G F, Skinner J T, Mundt H C and Williams R B. 2004. World Association for the Advancement of Veterinary Parasitology (WAAVP) guidelines for evaluating the efficacy of anticoccidial drugs in chickens and turkeys. Veterinary Parasitology 121(3-4):189–212. DOI: https://doi.org/10.1016/j.vetpar.2004.03.006
Hilbrich P. 1978. Krankheiten des Geflugels unter besondered Berucksichitigung der Haltung und Futterung. Hermann Kuhn KG, Schwenningen am Neckar, Germany.
Jenkins M C, O’Brien C N, Fuller L, Mathis G F and Fetterer R. 2014. A rapid method for determining salinomycin and monensin sensitivity in Eimeria tenella. Veterinary Parasitology 206(3-4):153–58. DOI: https://doi.org/10.1016/j.vetpar.2014.09.017
Johnson J and Reid W M. 1970. Anticoccidial drugs: Lesion scoring techniques in battery and floor-pen experiments with chickens. Experimental Parasitology 28(1): 30–36. DOI: https://doi.org/10.1016/0014-4894(70)90063-9
Kumar S, Garg R, Moftah A, Clark E L, Macdonald S E, Chaudhary A S, Sparagano O, Banerjee P S, Kundu K, Tomley F M and Blake D P. 2014. An optimised protocol for molecular identification of Eimeria from chickens. Veterinary Parasitology 199(1-2): 24–31. DOI: https://doi.org/10.1016/j.vetpar.2013.09.026
Kraieski A L, Salles G B C, Muniz E C, Nascimento D V J, Lima Neto A J, Santos I L and Madeira A M B N. 2021. Sensitivity of field isolates of Eimeria acervulina and E. maxima from three regions in Brazil to eight anticoccidial drugs. Poultry Science 100(8): 101233. DOI: https://doi.org/10.1016/j.psj.2021.101233
Metri R, D’Souza P E, Narasimhamurthy H N and Umakanta B. 2012. Efficacy of commonly used coccidiostats against experimental E. tenella infection in broiler chickens. Journal of Veterinary Parasitology 26: 53-56.
Peek H W and Landman W J M. 2011. Coccidiosis in Poultry: Anticoccidial Products, Vaccines and Other Prevention Strategies. Veterinary Quarterly 31:143-61. DOI: https://doi.org/10.1080/01652176.2011.605247
Stephan B, Rommel M, Daugschies A and Haberkorn A. 1997. Studies of resistance to anticoccidials in Eimeria field isolates and pure Eimeria strains. Veterianry Parasitology 69: 19–29. DOI: https://doi.org/10.1016/S0304-4017(96)01096-5
Tan L, Li Y, Yang X, Ke Q, Lei W, Mughal M N, Fang R, Zhou Y, Shen B and Zhao J. 2017. Genetic diversity and drug sensitivity studies on Eimeria tenella field isolates from Hubei Province of China. Parasites and Vectors 10: 137. DOI: https://doi.org/10.1186/s13071-017-2067-y
Yadav A and Gupta S K. 2001. Study of resistance against some ionophores in Eimeria tenella field isolates. Veterinary Parasitology 102(1-2): 69–75. DOI: https://doi.org/10.1016/S0304-4017(01)00512-X
Zhang J J, Wang L X, Ruan W K and An J. 2013. Investigation into the prevalence of coccidiosis and maduramycin drug resistance in chickens in China. Veterinary Parasitology 191(1-2): 29–34. DOI: https://doi.org/10.1016/j.vetpar.2012.07.027
Copyright (c) 2023 The Indian Journal of Animal Sciences
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
The copyright of the articles published in The Indian Journal of Animal Sciences is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.