A novel SNP (c.1311C>T) on heat shock protein 70 (HSP70) gene of Kacang goat in Indonesia

AHMAD FURQON; SUYADI SUYADI; KUSWATI KUSWATI; SUTIKNO SUTIKNO; FERDY SAPUTRA; WIKE ANDRE SEPTIAN

doi:10.56093/ijans.v93i7.132873

Authors

AHMAD FURQON National Research and Innovation Agency of the Republic of Indonesia, Bogor, Indonesia
SUYADI SUYADI University of Brawijaya, Malang, Indonesia https://orcid.org/0000-0002-9165-8873
KUSWATI KUSWATI University of Brawijaya, Malang, Indonesia https://orcid.org/0000-0002-0645-5888
SUTIKNO SUTIKNO National Research and Innovation Agency of the Republic of Indonesia, Bogor 16911, Indonesia
FERDY SAPUTRA National Research and Innovation Agency of The Republic of Indonesia, Bogor, Indonesia
WIKE ANDRE SEPTIAN University of Brawijaya, Malang, Indonesia https://orcid.org/0000-0002-3883-6730

https://doi.org/10.56093/ijans.v93i7.132873

Keywords:

DNA, Mutation, PCR, Polymorphism, Sequencing

Abstract

Heat shock proteins (HSPs) associated with stress reactions play an important role in cell survival by activating numerous regulatory proteins and inducing black apoptosis. This study aimed to identify the potential SNPs of HSP70 gene in Kacang goats in Indonesia. Forty-three Kacang goats were selected from Sidoarjo and Tulungagung districts. The DNA isolated from blood samples was successfully amplified using the polymerase chain reaction (PCR) method with a pair of primers. The PCR products were sequenced in the coding region. The sequences were successfully aligned to determine the potential SNPs. A novel SNP (c.1311C>T) was found in this study. This SNP was categorized as a synonymous mutation. The insertion-deletion (in-del) mutation was also observed at the 1151st
and 1161st nucleotide positions. Based on these mutations, four haplotypes were constructed where haplotype 1 had the highest frequency in Sidoarjo. Indonesian goats had no close relation with Iraqi goats according to Neighbor-Joing with Kimura’s 2-parameter approach. Haplotypes 1 and 2 in Indonesian goats had three different bases with all haplotypes in Iraqi goats in Median Joining Network. The study concluded that a novel SNP of HSP70 gene was identified in Kacang goats.

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