Carrier frequency of autosomal recessive disorders CVM and DUMPS in Sahiwal and Holstein Friesian cattle in Chhattisgarh

VINEET  KUMAR; ASIT  JAIN; TRIPTI  JAIN; KISHORE  MUKHERJEE; AAKANKSHA  RATHORE; KAISER  PARVEEN; FARHEEN  ANSARI

doi:10.56093/ijans.v94i6.132934

Authors

VINEET KUMAR Central Poultry Development Organisation, Northern Region, Chandigarh
ASIT JAIN Nanaji Deshmukh Veterinary Science University, Jabalpur, Madhya Pradesh
TRIPTI JAIN Nanaji Deshmukh Veterinary Science University, Jabalpur, Madhya Pradesh
KISHORE MUKHERJEE Dau Shri Vasudev Chandrakar Kamdhenu Vishwavidyalaya, Anjora, Durg, Chhattisgarh
AAKANKSHA RATHORE Livestock Development Department, Government of Chhattisgarh
KAISER PARVEEN Dau Shri Vasudev Chandrakar Kamdhenu Vishwavidyalaya, Anjora, Durg, Chhattisgarh
FARHEEN ANSARI Nanaji Deshmukh Veterinary Science University, Jabalpur, Madhya Pradesh

https://doi.org/10.56093/ijans.v94i6.132934

Keywords:

CVM, DUMPS, Genetic disorders

Abstract

In the present study, genetic screening of Sahiwal and Holstein Friesian (HF) cattle and its crosses was conducted using the PCR-RFLP technique to detect the presence of CVM and DUMPS disorders. Genomic DNA was extracted from 50 Sahiwal and 100 HF animals and its crosses using commercially available kits. Gene-specific primers were utilized for in vitro amplification of SLC35A3 and UMPS genes by PCR, followed by RFLP analysis using PstI and AvaI restriction endonucleases for CVM and DUMPS, respectively. Subsequently, the PCR products were purified and sequenced to confirm any mutations in the DNA target sequences. Notably, no affected or carrier animals for CVM and DUMPS disorders were identified among the screened animals. Thus, the gene and genotype frequency of CVM and DUMPS carriers was zero, indicating a 100% frequency of normal animals. The study underscores the importance of continued screening for these genetic disorders, involving a larger sample size across the state, and advocates for mandatory screening before utilizing animals for breeding purposes, in adherence to minimum standard protocols.

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