Clinicopathological alterations in subclinical ketosis in Chilika buffaloes, detected by patient-side urine test and influence of risk factors on prevalence

ASHISH  DORA; S K  SENAPATI; S  SATAPATHY; A P  ACHARYA; R C  PATRA

doi:10.56093/ijans.v95i10.133606

Authors

ASHISH DORA Odisha University of Agriculture and Technology, Bhubaneswar-751 003, India
S K SENAPATI Odisha University of Agriculture and Technology, Bhubaneswar-751 003, India
S SATAPATHY Odisha University of Agriculture and Technology, Bhubaneswar-751 003, India
A P ACHARYA Odisha University of Agriculture and Technology, Bhubaneswar-751 003, India
R C PATRA Odisha University of Agriculture and Technology, Bhubaneswar-751 003, India

https://doi.org/10.56093/ijans.v95i10.133606

Keywords:

Chilika buffaloes, Ketosis, Liver function, Milk, Rothera’s test

Abstract

The present study was carried out by recruiting 526 lactating Chilika buffaloes, kept in 29 herds, belonging to small and marginal farmers in 16 villages of three Chilika Lake-adjoining districts-Puri, Khordha and Ganjam, in the state of Odisha, India during the period from December 2019 to June 2020 to assess the prevalence of subclinical ketosis in their native tract, and to weigh the influence of risk factors, namely parity, lactation stage, and milk yield. All the buffaloes were screened by Rothera’s test on urine samples and Ross test on milk samples, that diagnosed subclinical ketosis in 41 animals, constituting 7.79% of the total tested buffaloes. Blood, milk and urine samples were collected from 20 lactating buffaloes, consisting of 10 apparently healthy buffaloes, and another ten with subclinical ketosis and analyzed for serum biochemical parameters, milk fat and solid not fat (SNF). The urine samples were tested using multi-diagnostic urinalysis strip. The highest prevalence of subclinical ketosis was observed in 4th parity (15.38%), followed by 3rd parity (11.20%), and in buffaloes yielding ≥ 3 kg of milk/ day (17.07%). The subclinical ketosis was more common during peak production, and was associated with significant decline in milk production, hepatic dysfunction and had sever hypoglycaemia, along with elevated mean serum triglycerides (mg/dl), activity of alanine aminotransferase (ALT), Aspartate Aminotransferase (AST). Serum calcium, magnesium and phosphorous levelS were significantly lower in buffaloes with subclinical ketosis than their apparently healthy counterparts.

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