Differential detection and molecular characterization of multiple avian oncogenic viruses in backyard poultry of Tamil Nadu, India

M  PRABHU; S  MALMARUGAN; N SWEETLINE  ANNE; S  GOPAL; K  GOPAL; V  KUMAR; J JOHNSON  RAJESWAR

doi:10.56093/ijans.v94.i1.134611

Authors

M PRABHU Tamil Nadu Veterinary and Animal Sciences University, Tirunelveli, Tamil Nadu 627 358 India
S MALMARUGAN Tamil Nadu Veterinary and Animal Sciences University, Tirunelveli, Tamil Nadu 627 358 India
N SWEETLINE ANNE Tamil Nadu Veterinary and Animal Sciences University, Tirunelveli, Tamil Nadu 627 358 India
S GOPAL Department of Animal Husbandry, Dairying and Fisheries, Tamil Nadu
K GOPAL Tamil Nadu Veterinary and Animal Sciences University, Tirunelveli, Tamil Nadu 627 358 India
V KUMAR Tamil Nadu Veterinary and Animal Sciences University, Tirunelveli, Tamil Nadu 627 358 India
J JOHNSON RAJESWAR Tamil Nadu Veterinary and Animal Sciences University, Tirunelveli, Tamil Nadu 627 358 India

https://doi.org/10.56093/ijans.v94.i1.134611

Keywords:

Mareks disease, Multiplex PCR, Native chicken, Oncogenic viruses, Phylogenetic analyses, Reticuloendotheliosis

Abstract

The avian oncogenic viruses include Mareks disease virus (MDV), Avian Leukosis virus (ALV) and Reticuloendotheliosis virus (REV). All the three viruses as etiological agents of single and/or multiple oncogenic infections were reported previously in broiler and layer chickens. The present study describes the detection of these multiple oncogenic viruses by multiplex PCR from suspected samples collected from the native chicken of Tamil Nadu, a southern state of India. Out of 51 samples tested, 10 were found positive for MDV and 25 positive for ALV subgroup E and 6 samples positive for REV. Further, the representative samples were subjected to sequencing and phylogenetic analysis. The molecular characterisation of the present MDV strains showed the close proximity with strains from Tamil Nadu, Punjab, and Kerala states of India with bootstrap value of 97%. Further, the current REV strain showed close relatedness with the previously known Tamil Nadu REV isolate with bootstrap value of 66%. The ALV strain identified from the backyard poultry was found to be endogenous virus and showed close identity with ALV-E China isolate with bootstrap value of 78%.

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References

Adams M J, Lefkowitz E J, King A M Q, Harrach B, Harrison R L, Knowles N J, Kropinski A M, Krupovic M, Kuhn J H and Mushegian A R. Ratification vote on taxonomic proposals to the International Committee on Taxonomy of viruses. Archives of Virology 161: 2921–949.

Adedeji A J, Akanbi O B, Luka P D and Abdu P. 2019. Natural outbreak of Marek’s disease in indigenous chicken and Japanese quail (Coturnix coturnix japonica) in Jos, Plateau State, Nigeria. Open Veterinary Journal 9(2):151–56.

Balachandran C, Pazhanivel N, Vairamuthu S and Murali M B. 2009. Marek’s disease and lymphoid leucosis in chicken-A histopathological survey. Tamil Nadu Journal of Veterinary and Animal Sciences 5: 167–70.

Balasubramaniam A, Saravanajayam M and Arulmozhi A. 2017. Recurrent incidence of Marek’s disease in native breed chickens. Journal of Animal Research 7(4): 789–91.

Boodhoo N, Gurung A, Sharif S and Behboudi S. 2016. Marek’s disease in chickens: A review with focus on immunology. Veterinary Research 47: 119.

Chacón R D, Astolfi-Ferreira C S, Guimarães M B, Torres L N, De la Torre D I, Sá L R M and Piantino Ferreira A J. 2019. Detection and molecular characterization of a natural coinfection of Marek’s disease virus and Reticuloendotheliosis virus in Brazilian Backyard chicken ﬂock. Veterinary Sciences 6(4): 92.

Davidson I. 2005. Veiled and unveiled aspects of infection with oncogenic viruses. Keynote lecture article. Proceedings of the 14th World Veterinary Poultry Congress, Istanbul, Turkey.

Davidson I. 2009. The knowledge that human tumor virology can gain from studies on avian tumor viruses. Advances in Tumor Virology 1: 9 –19.

Davidson I and Borenstein R. 1999. Multiple infection of Chickens and Turkeys with Avian oncogenic viruses: Prevalence and molecular analysis. Acta Virologica 43: 136–142.

Duguma R, Yami A, Dana N, Hassen F and Esatu W. 2005. Marek’s disease in local chicken strains of Ethiopia reared under confined management regime in central Ethiopia. Revue de Médecine Vétérinaire 156(11): 541–46.

Fadly A M and Venugopal K. 2008. Leukosis/sarcoma group, pp. 514–568. (Ed) Saif Y M. Diseases of Poultry, 12th ed. Iowa State Press, Ames, IA.

Fadly A M and Witter R L. 1997. Comparative evaluation of in vitro and in vivo assays for the detection of reticuloendotheliosis virus as a contaminant in a live vaccine of poultry. Avian Diseases 41: 695–701.

Gopal S, Manoharan P, Kathaperumal K, Chidambaram B and Divyaa K C. 2012. Differential detection of avian oncogenic viruses in poultry layer farms and turkeys by use of multiplex PCR. Journal of Clinical Microbiology 50: 2668–673.

Mescolini G, Lupini C, Felice V, Cecchinato M, Guerrini A and Catelli E. 2019. Molecular characterization of the meq gene of Marek’s disease viruses detected in unvaccinated backyard chickens reveals the circulation of low- and high- virulence strains. Poultry Science 98(8): 3130–137.

Payne L N. 1991. Developments in avian leukosis research. Leukemia 6: 150S–152S.

Prathibha Y, Sreedevi B, Kumar N V and Srilatha C H. 2018. Molecular characterization and phylogenetic analysis of oncogenes from virulent serotype-1 Marek’s disease virus in India. Acta Virologica 62(3): 277–86.

Puro K, Bhattacharjee U, Baruah S, Sen, Das S, Ghatak S, Doley S, Sanjukta R and Shakuntala I. 2018. Characterization of Marek’s disease virus and phylogenetic analyses of meq gene from an outbreak in poultry in Meghalaya of Northeast India. Virus Disease 29(2): 167–72.

Qin L T, Gao Y L, Pan W, Deng X Y, Sun F, Li K, Qi X, Gao H, Liu C and Wang X. 2010. Investigation of co-infection of ALV-J with REV, MDV, CAV in layer chicken ﬂocks in some regions of China. Chinese Journal of Preventive Veterinary Medicine 32(2): 90–93.

Raja A, Dhinakar Raj G, Bhuvaneswari P, Balachandran C and Kumanan K. 2009. Detection of virulent Marek’s disease virus in poultry in India. Acta Virologica 53(4): 255–60.

Silva R F, Fadly A M and Taylor S P. 2007. Development of a polymerase chain reaction to differentiate avian leukosis virus (ALV) subgroups: Detection of an ALV contaminant in commercial Marek’s disease vaccines. Avian Diseases 51: 663–67.

Smith E J and Fadly A M. 1988. Inﬂuence of congenital transmission of endogenous virus-21 on the immune response to avian leukosis virus infection and the incidence of tumors in chickens. Poultry Science 67: 1674–679.

Sun G R, Zhang Y P, Lv H C, Zhou L Y, Cui H Y, Gao Y L, Qi X L, Cui H Y, Wang Y Q and Gao L. 2017. Co-infection with Marek’s disease virus and Reticuloendotheliosis virus increases illness severity and reduces marek’s disease vaccine efficacy. Viruses 9(158): 1–15.

Suresh P, Johnson Rajeswar J, Sukumar K, Harikrishnan T J and Srinivasan P. 2015. Pathotyping of recent Indian field isolates of Marek’s disease virus serotype 1. Acta Virologica 59(2): 156–65.

Witter R L and Fadly A M. 2003. Reticuloendotheliosis, pp. 517–35. (Ed) Saif Y M. Diseases of Poultry, 11th ed Iowa State University Press, Ames, IA.

Zavala G and Cheng S. 2006. Detection and characterization of avian leukosis virus in Marek’s disease vaccines. Avian Diseases 50: 209–15.