Prevalence, extended-spectrum β-lactamase and biofilm production ability ofEscherichia coli isolated from buffalo mastitis

SARITA  YADAV; PARVINA DEVI; ASHOK  BOORA; NISHU; PRADEEP KUMAR; AMANDEEP; RAJESH KUMAR  LOHCHAB; ANIL KUMAR

doi:10.56093/ijans.v93i12.138397

Authors

SARITA YADAV ICAR-Central Institute for Research on Buffaloes, Hisar, Haryana 125 001 India
PARVINA DEVI Lovely Professional University, Punjab
ASHOK BOORA ICAR-Central Institute for Research on Buffaloes, Hisar, Haryana 125 001 India
NISHU ICAR-Central Institute for Research on Buffaloes, Hisar, Haryana 125 001 India
PRADEEP KUMAR ICAR-Central Institute for Research on Buffaloes, Hisar, Haryana 125 001 India
AMANDEEP ICAR-Central Institute for Research on Buffaloes, Hisar, Haryana 125 001 India
RAJESH KUMAR LOHCHAB Guru Jambheshwar University of Science and Technology, Hisar, Haryana
ANIL KUMAR Guru Jambheshwar University of Science and Technology, Hisar, Haryana.

https://doi.org/10.56093/ijans.v93i12.138397

Keywords:

Antimicrobial susceptibility test, Buffalo, Biofilm, E. coli, Farmer FIRST, Mastitis

Abstract

This study aimed to determine the prevalence, antibiotic resistance pattern, extended-spectrum, beta-lactamase production and biofilm forming ability of isolated E. coli strains from buffaloes mastitis milk. Out of 549 bacterial isolates from mastitis milk of buffaloes (n, animal level= 472) between 2019 and 2022, a total of 43 E. coli strains were isolated with an overall prevalence of 9.11% at animal level. Prevalence of E coli was high in unorganised buffalo herd (11.36%) from villages of Farmer FIRST project (ICAR-FFP) compared with organised buffalo farms (6.73%). The highest resistance was against Penicillin 43 (100%) followed by Ceftriaxone 18 (41.86%), Amoxycillin/Sulbactam 8 (18.60%) and Enrofloxacin 7 (16.27%). Additionally, all were sensitive to gentamycin 43 (100%) followed by Cefoperazone/Sulbactam 34 (79.06%). Cephalosporins are frequently used antibiotics to treat bovine mastitis. However, their therapeutic effectiveness is being compromised by bacterial resistant to β-lactams. In present study, a total of 32 (6.78% %) extended-spectrum β-lactamase (ESBL) producing E coli were isolated from mastitic buffalo milk (n=43/472). In total, 17 (39.5%) isolates were biofilm producers by microtiter-plate method. There was statistically non-significant relationship between biofilm production and antibiotic resistance as well as between ESBL production and biofilm formation in E coli strains. Present study demonstrated a high occurrence of ESBL and biofilm producing E. coli in buffalo mastitis milk, implementing a significant challenge to treat mastitis in buffaloes, necessitates judicious use of antimicrobials and to explore potential therapeutic agents as substitutes for
antibiotics to treat bovine mastitis effectively.

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