Prevalence, extended-spectrum β-lactamase and biofilm production ability ofEscherichia coli isolated from buffalo mastitis
DOI:
https://doi.org/10.56093/ijans.v93i12.138397Keywords:
Antimicrobial susceptibility test, Buffalo, Biofilm, E. coli, Farmer FIRST, MastitisAbstract
This study aimed to determine the prevalence, antibiotic resistance pattern, extended-spectrum, beta-lactamase production and biofilm forming ability of isolated E. coli strains from buffaloes mastitis milk. Out of 549 bacterial isolates from mastitis milk of buffaloes (n, animal level= 472) between 2019 and 2022, a total of 43 E. coli strains were isolated with an overall prevalence of 9.11% at animal level. Prevalence of E coli was high in unorganised buffalo herd (11.36%) from villages of Farmer FIRST project (ICAR-FFP) compared with organised buffalo farms (6.73%). The highest resistance was against Penicillin 43 (100%) followed by Ceftriaxone 18 (41.86%), Amoxycillin/Sulbactam 8 (18.60%) and Enrofloxacin 7 (16.27%). Additionally, all were sensitive to gentamycin 43 (100%) followed by Cefoperazone/Sulbactam 34 (79.06%). Cephalosporins are frequently used antibiotics to treat bovine mastitis. However, their therapeutic effectiveness is being compromised by bacterial resistant to β-lactams. In present study, a total of 32 (6.78% %) extended-spectrum β-lactamase (ESBL) producing E coli were isolated from mastitic buffalo milk (n=43/472). In total, 17 (39.5%) isolates were biofilm producers by microtiter-plate method. There was statistically non-significant relationship between biofilm production and antibiotic resistance as well as between ESBL production and biofilm formation in E coli strains. Present study demonstrated a high occurrence of ESBL and biofilm producing E. coli in buffalo mastitis milk, implementing a significant challenge to treat mastitis in buffaloes, necessitates judicious use of antimicrobials and to explore potential therapeutic agents as substitutes for
antibiotics to treat bovine mastitis effectively.
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References
Bandyopadhyay S, Banerjee J, Bhattacharyya D, Samanta I, Mahanti A, Dutta T K, Ghosh S, Nanda P K, Dandapat P and Bandyopadhyay S. 2018. Genomic identity of fluoroquinolone-resistant bla CTX-M-15-Type ESBL and pMAmpC β-lactamase producing Klebsiella pneumoniae from buffalo milk, India. Microbial Drug Resistance 24(9): 1345–53. DOI: https://doi.org/10.1089/mdr.2017.0368
Bhanot V, Chaudhri S, Bisla R and Singh H. 2012. Retrospective study on prevalence and antibiogram of mastitis in cows and buffaloes of eastern Haryana. Indian Journal of Animal Research 46(2): 160–63.
Bhat A M, Soodan J S, Singh R, Dhobi I A, Hussain T, Dar M Y and Mir M. 2017. Incidence of bovine clinical mastitis in Jammu region and antibiogram of isolated pathogens. Veterinary World 10(8): 984. DOI: https://doi.org/10.14202/vetworld.2017.984-989
Bisht K S, Mishra R, Pati P K, Patra R, Rath P K and Sahoo P R. 2020. Identification and characterization of Escherichia coli isolates in bovine mastitis milk from Bhubaneswar, Odisha, India. International Journal of Current Microbiology and Applied Sciences 9(9): 2088–95. DOI: https://doi.org/10.20546/ijcmas.2020.909.260
Charaya G, Sharma A, Kumar A, Singh M and Goel P. 2014. Pathogens isolated from clinical mastitis in Murrah buffaloes and their antibiogram. Veterinary World 7(11). DOI: https://doi.org/10.14202/vetworld.2014.980-985
CLSI. 2012. Performance standards for antimicrobial susceptibility testing; twenty-second informational supplement. CLSI document M100.
Das A, Guha C, Biswas U, Jana P S, Chatterjee A and Samanta I. 2017. Detection of emerging antibiotic resistance in bacteria isolated from subclinical mastitis in cattle in West Bengal. Veterinary World 10(5): 517. DOI: https://doi.org/10.14202/vetworld.2017.517-520
Das D, Chakrabarty P, Saha S, Pal N and Bhattacharya S. 2021. A cross-sectional study on association between antibiotic resistance pattern and biofilm production of E. coli in non-catheterised UTI patients at a tertiary care hospital in Kolkata. DOI: https://doi.org/10.18410/jebmh/2021/106
Di Domenico E G, Farulla I, Prignano G, Gallo M T, Vespaziani M, Cavallo I, Sperduti I, Pontone M, Bordignon V and Cilli L. 2017. Biofilm is a major virulence determinant in bacterial colonization of chronic skin ulcers independently from the multidrug resistant phenotype. International Journal of Molecular Sciences 18(5): 1077. DOI: https://doi.org/10.3390/ijms18051077
Farooq A, Inayat S, Akhtar M and Mushtaq M. 2008. Prevalence of mastitis and antibiotic sensitivity of bacterial isolates recovered from Nili-Ravi buffaloes. Journal of Animal and Plant Sciences 18: 2–3.
Heikkilä A-M, Liski E, Pyörälä S and Taponen S. 2018. Pathogen-specific production losses in bovine mastitis. Journal of Dairy Science 101(10): 9493–504. DOI: https://doi.org/10.3168/jds.2018-14824
Jarlier V, Nicolas M-H, Fournier G and Philippon A. 1988. Extended broad-spectrum β-lactamases conferring transferable resistance to newer β-lactam agents in Enterobacteriaceae: Hospital prevalence and susceptibility patterns. Clinical Infectious Diseases 10(4): 867–78. DOI: https://doi.org/10.1093/clinids/10.4.867
Kayastha K, Dhungel B, Karki S, Adhikari B, Banjara M R, Rijal K R and Ghimire P. 2020. Extended-spectrum β-lactamase-producing Escherichia coli and Klebsiella species in pediatric patients visiting International Friendship Children’s Hospital, Kathmandu, Nepal. Infectious Diseases: Research and Treatment 13: 1178633720909798. DOI: https://doi.org/10.1177/1178633720909798
Kuralayanapalya S P, Patil S S, Hamsapriya S, Shinduja R, Roy P and Amachawadi R G. 2019. Prevalence of extended-spectrum beta-lactamase producing bacteria from animal origin: A systematic review and meta-analysis report from India. PLoS One 14(9): e0221771. DOI: https://doi.org/10.1371/journal.pone.0221771
Madani A, Esfandiari Z, Shoaei P and Ataei B. 2022. Evaluation of Virulence Factors, Antibiotic Resistance, and Biofilm Formation of Escherichia coli Isolated from Milk and Dairy Products in Isfahan, Iran. Foods 11(7): 960. DOI: https://doi.org/10.3390/foods11070960
Méric G, Hitchings M D, Pascoe B and Sheppard S K. 2016. From Escherich to the Escherichia coli genome. The Lancet Infectious Diseases 16(6): 634–36. DOI: https://doi.org/10.1016/S1473-3099(16)30066-4
Neupane S, Pant N D, Khatiwada S, Chaudhary R and Banjara M R. 2016. Correlation between biofilm formation and resistance toward different commonly used antibiotics along with extended spectrum beta lactamase production in uropathogenic Escherichia coli isolated from the patients suspected of urinary tract infections visiting Shree Birendra Hospital, Chhauni, Kathmandu, Nepal. Antimicrobial Resistance and Infection Control 5(1): 5. DOI: https://doi.org/10.1186/s13756-016-0104-9
Pedersen R R, Krömker V, Bjarnsholt T, Dahl-Pedersen K, Buhl R and Jørgensen E. 2021. Biofilm research in bovine mastitis. Frontiers in Veterinary Science: 449. DOI: https://doi.org/10.3389/fvets.2021.656810
Quinn P J, Carter M E, Markey B and Carter G R. 1994. Clinical Veterinary Microbiology. Wolf/Mosby, London, England
Sharma A, Chhabra R, Singh M and Charaya G. 2018. Prevalence, etiology and antibiogram of bacterial isolates recovered from mastitis of buffaloes. Buffalo Bulletin 37(3): 313–20.
Singh A, Chhabra D, Sikrodia R, Shukla S, Sharda R and Audarya S. 2018. Isolation of E. coli from bovine mastitis and their antibiotic sensitivity pattern. International Journal of Current Microbiology and Applied Sciences 7(10): 11–18. DOI: https://doi.org/10.20546/ijcmas.2018.710.002
Stepanović S, Vuković D, Dakić I, Savić B and Švabić-Vlahović M. 2000. A modified microtiter-plate test for quantification of staphylococcal biofilm formation. Journal of microbiological methods 40(2): 175–79. DOI: https://doi.org/10.1016/S0167-7012(00)00122-6
Sumathi B, Veeregowda B and Amitha R G. 2008. Prevalence and antibiogram profile of bacterial isolates from clinical bovine mastitis. Veterinary World 1(8): 237–38.
Sweeney M T, Lubbers B V, Schwarz S and Watts J L. 2018. Applying definitions for multidrug resistance, extensive drug resistance and pandrug resistance to clinically significant livestock and companion animal bacterial pathogens. Journal of Antimicrobial Chemotherapy 73(6): 1460-63. DOI: https://doi.org/10.1093/jac/dky043
Wayne P. 2010. Clinical and Laboratory Standards Institute: Performance standards for antimicrobial susceptibility testing: 20th informational supplement. CLSI Document: M100-S20.
Yadav V, Joshi R, Joshi N and Singh S. 2022. Status of multidrug resistance among ESBL producing E. coli and Klebsiella spp. isolates of buffalo origin in eastern plain zone of Uttar Pradesh. Haryana Veterinarian 60(2): 208–12. DOI: https://doi.org/10.18805/IJAR.B-5036
Yang F, Zhang S, Shang X, Wang L, Li H and Wang X. 2018. Characteristics of quinolone-resistant Escherichia coli isolated from bovine mastitis in China. Journal of Dairy Science 101(7): 6244–52. DOI: https://doi.org/10.3168/jds.2017-14156
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