Isolation and molecular characterization of Lumpy skin disease virus fromcattle and the detection of anti-viral antibodies in buffaloes

SARITA  YADAV; ASHOK  BOORA; PARVINA  DEVI; NISHU; NISHA  VERMA; INDERJEET  SINGH; ANIL  KUMAR

doi:10.56093/ijans.v94.i1.138729

Authors

SARITA YADAV ICAR–Central Institute for Research on Buffaloes, Hisar, Haryana 125 001 India
ASHOK BOORA ICAR–Central Institute for Research on Buffaloes, Hisar, Haryana 125 001 India
PARVINA DEVI ICAR–Central Institute for Research on Buffaloes, Hisar, Haryana 125 001 India
NISHU ICAR–Central Institute for Research on Buffaloes, Hisar, Haryana 125 001 India
NISHA VERMA ICAR–Central Institute for Research on Buffaloes, Hisar, Haryana 125 001 India
INDERJEET SINGH Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab
ANIL KUMAR Indian Council of Agricultural Research, New Delhi

https://doi.org/10.56093/ijans.v94.i1.138729

Keywords:

Buffalo, Cattle, ICAR- Farmer FIRST, Lumpy skin disease

Abstract

In the present study, four LSDV field strains were isolated from cattle in Bhadra tehsil, Hanumangarh district, Rajasthan under ICAR-Farmer FIRST Project and Bathinda, Punjab during the period 2021 and 2022. Apart from the characteristic clinical signs of LSD, the viral etiology was confirmed by PCR targeting LSDV074, LSDV 117 and LSDV036 genes, in addition to characteristic clinical signs LSD in cattle. Though, buffalo is a susceptible host, only mild deep seated skin nodules suspected for LSD were reported from in-contact buffaloes under field condition in this study. None of the samples from buffalo were found positive for LSDV, BHV-1 and BHV-2 DNA by PCR. Buffaloes exhibiting mild clinical signs in the current study showed an antibody titre of 1:16 to 1:64 by virus neutralization assay. No information is available regarding the seroprevalence of LSD in buffalo from India, therefore, it is imperative to further investigate the susceptibility of buffalo to LSDV infection. Based on the phylogenetic analysis of the ORF036 gene sequence, LSDV/India/RAJ/ OP604055/2021 was in close relationship with LSDV from India, Kenya, Nepal, Bangladesh and Myanmar as they clustered together as a sub-cluster.

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