Prolactin gene (C576A) polymorphism is associated with milk production performance in crossbred Anglo-Nubian dairy goats
![](/public/icon/gor.png)
![](/public/icon/pdf.png)
Keywords:
Anglo-Nubian, Milk yield, PCR-RFLP, Polymorphism, ProlactinAbstract
Crossbred Anglo-Nubian goats (Anglo-Nubian × native goat breeds), are valued for exceptional milk production and adaptability. In-depth research on these goats is imperative to propel advancements in dairy goat breeds and enhance milk production efficiency within the region. The present study aims to assess polymorphism in prolactin (C576A) gene and determine the influence of different prolactin (PRL) genotypes to milk yield performance in crossbred Anglo-Nubian dairy goats raised from Awang, Opol, Misamis Oriental and Talay, Dumaguete City, Negros Oriental. Genomic DNA was extracted from hair follicles using commercial DNA extraction kit and PCR-RFLP was performed for the genotyping of the C576A polymorphism located in exon 5 of goats’ prolactin gene using Eco241 restriction enzyme. Genotypic frequencies of 0.56 for AA, 0.44 for AB, while allelic frequencies of 0.78 for A, and 0.22 for B were recorded. All populations followed the Hardy–Weinberg principle, except for dairy goats from Farm A located in Opol, Misamis Oriental. A two-way factorial (2 × 4) in a Randomized Complete Block Design was used to evaluate the relationship between genotypes and milk yield performance. AB genotype goats produced significantly higher milk yield traits (average daily milk yield and total milk production) than AA genotype, an indication that the polymorphism in the caprine PRL (C576A) gene influenced milk yield performance in the population of studied crossbred Anglo-Nubian goats. These results have to be validated in other dairy goat breeds.
Downloads
References
Abbas H M, Hassan F A M, El-Gawad M A M and Enab A K. 2014. Physicochemical characteristics of goat’s milk. Life Science Journal 11(1): 307–17.
Abdel-Aziem S H, Mahrous K F, El-Hafez M A and Mordy M A. 2018. Genetic variability of myostatin and prolactin genes in popular goat breeds in Egypt. Journal of Genetic Engineering and Biotechnology 16(1): 89–97.
Alipanah M, Kalashnikova L and Rodionov G. 2007. Association of prolactin gene variants with milk production traits in Russian Red Pied cattle. Iranian Journal of Biotechnology 5(3): 158–61.
Al-Samarai F R and Al-Kazaz A A. 2015. Applications of molecular markers in animal breeding. American Journal of Applied Scientific Research 1(1): 1–5.
Aravindakshan T V. 2005. Polymorphism at K-casein and prolactin loci in goats investigatyed by PCR-RFLP and DNA sequencing. Indian Veterinary Journal 82: 126–30.
Chung E R, Rhim T J and Han S K. 1996. Associations between PCR-RFLP markers of growth hormone and prolactin genes and production traits in dairy cattle. Korean Journal of Animal Science 38: 321–36.
Getahun D, Alemneh T, Akeberegn D, Getabalew M and Zewdie D. 2019. Importance of hybrid vigor or heterosis for animal breeding. Biochemestry Biotechnology Research 7: 1–4.
Ghasemi N, Zadehrahmani M, Rahimi G and Hafezian S H. 2009. Associations between prolactin gene polymorphism and milk production in Montebeliard cows. International Journal of Genetics and Molecular Biology 1(3): 048–051.
Gomez K A and Gomez A A. 1984. Statistical Procedures for Agricultural Research. John Wiley & Sons.
Hart G L, Bastiaansen J, Dentine M R and Kirkpatrick B W. 1993. Detection of a four allele single strand conformation polymorphism (SSCP) in the bovine prolactin gene 5 ‘flank. Animal Genetics 24(2): 149.
Idowu S T and Adewumi O O. 2017. Genetic and non-genetic factors affecting yield and milk composition in goats. Journal of Advance Dairy Research 5(2): 1–4.
Lan X Y, Pan C Y, Chen H, Lei C Z, Li F Y, Zhang H Y and Ni Y S. 2009. Novel SNP of the goat prolactin gene (PRL) associated with cashmere traits. Journal of Applied Genetics 50(1): 51–54.
Li J, Liang A, Li Z, Du C, Hua G, Salzano A, Campanile G, Gasparrini B and Yang L. 2017. An association analysis between PRL genotype and milk production traits in Italian Mediterranean river buffalo. Journal of Dairy Research 84(4): 430–33.
National Dairy Authority. 2017. Philippine Dairy Update Jan. – Dec. 2017. http://www.nda.da.gov.ph/images/2017/data/Phil%20%20DAiry%20Update%202017.pdf
Pizarro Inostroza M G, Landi V, Navas González F J, León Jurado J M, Delgado Bermejo J V, Fernández Álvarez J and Martínez Martínez M D A. 2020. Integrating casein complex SNPs additive, dominance and epistatic effects on genetic parameters and breeding values estimation for murciano- granadina goat milk yield and components. Genes 11(3): 309.
Shamsalddini S, Mohammadabadi M R and Esmailizadeh A K. 2016. Polymorphism of the prolactin gene and its effect on fiber traits in goat. Russian Journal of Genetics 52(4): 405–08.
Singh P, Singh M K, Rout P K and Dige M S. 2018. Association of the CSN1S1 gene polymorphism with milk composition traits in Jamunapari goat. Indian Journal of Animal Research 52(8).
Turkington R W, Frantz W I and Majumder G C.1973. Effector- receptor relations in the action of prolactin. Human prolactin, Excepta Medica Amsterdam, 24 p.
USDA, United States Department of Agriculture. 2020. Foreign Agricultural Service. Annual Dairy and Products Report. October 14, 2020.
Vellend M and Geber M A. 2005. Connections between species diversity and genetic diversity. Ecology Letters 8(7): 767–81.
Wallis M. 1988. Mechanism of action of growth hormone. New Comprehensive Biochemistry 18: 265–94.
Zhang H, Denise S K and Ax R L. 1994. Rapid communication: Diallelic single-stranded conformational polymorphism detected in the bovine prolactin gene. Journal of Animal Science 72(1): 256–56.
Downloads
Submitted
Published
Issue
Section
License
Copyright (c) 2024 The Indian Journal of Animal Sciences
![Creative Commons License](http://i.creativecommons.org/l/by-nc-sa/4.0/88x31.png)
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
The copyright of the articles published in The Indian Journal of Animal Sciences is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.