Nationwide cross-sectional study on brucellosis sero-prevalence in buffaloes using competitive ELISA

RAJESWARI  SHOME; SHARANGOUDA  PATIL; SAMER  SHAMSHAD; SOMY  SKARIAH; MUNEERA MOHAMED  SAHIB; G  SHANMUGAM; NAGENDRA NATH  BARMAN; DURLAV PRASAD  BORA; ARIJIT  SHOME

doi:10.56093/ijans.v94i12.142684

Authors

RAJESWARI SHOME ICAR-National Institute of Veterinary Epidemiology and Disease Informatics, Yelahanka, Bengaluru, Karnataka 560 064 India
SHARANGOUDA PATIL ICAR-National Institute of Veterinary Epidemiology and Disease Informatics, Yelahanka, Bengaluru, Karnataka 560 064 India
SAMER SHAMSHAD ICAR-National Institute of Veterinary Epidemiology and Disease Informatics, Yelahanka, Bengaluru, Karnataka 560 064 India
SOMY SKARIAH ICAR-National Institute of Veterinary Epidemiology and Disease Informatics, Yelahanka, Bengaluru, Karnataka 560 064 India
MUNEERA MOHAMED SAHIB ICAR-National Institute of Veterinary Epidemiology and Disease Informatics, Yelahanka, Bengaluru, Karnataka 560 064 India
G SHANMUGAM ICAR-National Institute of Veterinary Epidemiology and Disease Informatics, Yelahanka, Bengaluru, Karnataka 560 064 India
NAGENDRA NATH BARMAN College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, Assam
DURLAV PRASAD BORA College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, Assam
ARIJIT SHOME College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, Assam

https://doi.org/10.56093/ijans.v94i12.142684

Keywords:

Bubalus bubalis, Brucellosis, cELISA, India, Sero-prevalence

Abstract

Brucellosis is a bacterial disease caused by various Brucella species, which mainly infect bovines (cattle, buffalo), small ruminants (sheep, goat), swine, and dogs and humans. Earlier studies have reported brucellosis sero-prevalence only in cattle or both in cattle and buffaloes (bovine brucellosis) and actual disease burden in buffaloes was not available. The current study aimed to record countrywide brucellosis sero-prevalence in Indian buffaloes using competitive ELISA (cELISA). For the study, 1086 female buffalo (Bubalus bubalis) serum samples were collected from the top 10 states of India having the highest buffalo population. Overall, samples were drawn from 62 districts, 104 blocks and 242 epiunits from five regions of the country ({Punjab-100, Haryana-109, Uttar Pradesh-101, Rajasthan-100, Gujarat-156, Andhra Pradesh-100, Madhya Pradesh-110, Maharashtra-108, Karnataka-102, Andhra Pradesh-100 and Tamil Nadu-100)}. Samples were tested for anti-brucella antibodies using in-house developed monoclonal-based competitive ELISA (cELISA). Overall, apparent prevalence (AP) of 15.38% (CI-95%;13.35-17.64) and true prevalence (TP) of 15.85% (CI-95%; 13.77-18.19) were recorded. Sero-prevalence was highest in Punjab state (AP:57%; 47.22-66.27) in the Northern region and lowest in Madhya Pradesh (AP:0.90; 0.16-4.97) state of Central region with some of the districts displaying up to 90% and 70%, seropositivity in the Punjab state. Low brucellosis prevalence was noted in young (2.1 to 5 years) and older age groups of animals (11.1 to 13 years) and significantly high in the age group between 8 to 11 years (32.23%). The highest brucellosis seropositivity was observed in buffalo breeds such as Nagpuri and Murrah (28.79% and 20.67%), respectively and significant association was noted among four breeds. In conclusion, India has the highest buffalo population with very high-yielding buffalo breeds (109.85 million). Periodical surveillance is essential to detect and control brucellosis in buffaloes.-the pride species of the country.

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References

Aulakh H K, Patil P K, Sharma S, Kumar H, Mahajan V and Sandhu K S. 2008. A study on the epidemiology of bovine brucellosis in Punjab (India) using milk-ELISA. Acta Veterinaria Bruno 77(3): 393–99. DOI: https://doi.org/10.2754/avb200877030393

Dadar M, Shahali Y and Whatmore A M. 2019. Human brucellosis caused by raw dairy products: A review on the occurrence, major risk factors and prevention. International Journal of Food Microbiology 292(3): 39–47. DOI: https://doi.org/10.1016/j.ijfoodmicro.2018.12.009

Das V M, Paranjape V L and Corbel M J. 1990. Investigation of brucellosis-associated abortion in dairy buffaloes and cows in Bombay. The Indian Journal of Animal Sciences 60(10): 119–94.

Dhand N K, Gumber S, Singh B B, Aradhana, Bali M S, Kumar H, Sharma D R, Singh J and Sandhu K S. 2005. A study on the epidemiology of brucellosis in Punjab (India) using survey toolbox. Revue scientifique et technique (International Office of Epizootics) 24(3): 879–85. DOI: https://doi.org/10.20506/rst.24.3.1614

Holt H R, Bedi J S, Kaur P, Mangtani P, Sharma N S, Gill J P S, Singh Y, Kumar R, Kaur M, McGiven J and Guitian J. 2021. Epidemiology of brucellosis in cattle and dairy farmers of rural Ludhiana, Punjab. PLoS Neglected Tropical Diseases 15(3): e0009102. DOI: https://doi.org/10.1371/journal.pntd.0009102

Jagapur R V, Rathore R, Karthik K and Somavanshi R. 2013. Seroprevalence studies of bovine brucellosis using indirect- enzyme-linked immunosorbent assay (i-ELISA) at organized and unorganized farms in three different states of India. Veterinary World 6(8): 550–53. DOI: https://doi.org/10.5455/vetworld.2013.550-553

Jain U, Bist B and Dwivedi K. 2013. Outbreak of brucellosis in buffaloes aborted in village Mahuan, district Mainpuri, U.P., India- A case report. Veterinary World 6(1): 51–52. DOI: https://doi.org/10.5455/vetworld.2013.51-52

Jin L Z, Wang F and Wan C Y. 2017. Discussion on the prevention and control of brucellosis among livestock in Songyuan City. Friends Farmers Rich 61: 249–50.

Jindal P, Singh B B, Kaur P and Gill J P S. 2017. Sero-prevalence and molecular detection of Brucella species in slaughter pigs (Sus scrofa) of Punjab. India. Journal of Animal Research 7(3): 495–99. DOI: https://doi.org/10.5958/2277-940X.2017.00073.0

Jonsson E. 2013. Seroprevalence and risk factors for bovine brucellosis, salmonellosis and bovine viral diarrhea in urban and periurban areas of Kampala, Uganda. Swedish University of Agricultural Sciences, Uppsala, Sweden. http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-s-2002 (Accessed 31 January 2020).

Kalleshamurthy T, Skariah S, Rathore Y, Ramanjinappa K D, Nagaraj C, Shome B R, Rahman H, Barman N N and Shome R. 2020. Comparative evaluation of fluorescence polarization assay and competitive ELISA for the diagnosis of bovine brucellosis vis-a-vis sero-monitoring. Journal of Microbiological Methods 170(1): 105858–63. DOI: https://doi.org/10.1016/j.mimet.2020.105858

Kant N, Kulshreshtha P, Singh R, Mal A, Dwivedi A, Ahuja R, Mehra R, Tehlan M, Ahmed P, Kaushik S, Shipra, Kumar S, Mohammad A, Shukla S, Singh D and Bhatnagar R. 2018. A study to identify the practices of the buffalo keepers which inadvertently lead to the spread of brucellosis in Delhi. BMC Veterinary Research 14(1): 329–37. DOI: https://doi.org/10.1186/s12917-018-1670-2

Khurana S K, Srivastava S K and Krishnamsetty P. 2012. Seroprevalence of bovine brucellosis in Haryana by avidin- biotin serum ELISA and its comparison with RBPT and SAT. The Indian Journal of Animal Sciences 82(5): 448–0.

Kumar R V S, Lakshmi N D, Veena P, Sankar P and Yasotha P. 2010. Surgical management of cervical esophageal obstruction in a buffalo: A case report. Buffalo Bulletin 29(2): 71–72.

Kumar V and Gupta J. 2018. Prevailing practices in the use of antibiotics by dairy farmers in Eastern Haryana region of India. Veterinary World 11(3): 274–80. DOI: https://doi.org/10.14202/vetworld.2018.274-280

Malik R, Gursimran F and Mohinder P G. 2018. Seroprevalence of brucellosis in buffaloes by indirect enzyme linked immune- sorbent assay in Punjab, India. International Journal of Livestock Research 8(6): 244–50. DOI: https://doi.org/10.5455/ijlr.20170915054130

Mehra K N, Dhanesar N S and Chaturvedi V K. 2000. Sero– prevalence of brucellosis in bovines of Madhya Pradesh. Indian Veterinary Journal 77(7): 571–73.

Megersa B, Biffa D, Abunna F, Regassa A, Godfroid J and Skjerve E. 2011. Seroprevalence of brucellosis and its contribution to abortion in cattle, camel, and goat kept under pastoral management in Borana, Ethiopia. Tropical Animal Health and Production 43(3): 651–56. DOI: https://doi.org/10.1007/s11250-010-9748-2

Moreno E. 2014. Retrospective and prospective perspectives on zoonotic brucellosis. Frontiers in Microbiology 5(5): 213–30. Naveenkumar V, Bharathi V, Kannan P and Muthukrishnan S. 2017. Serological studies on bovine brucellosis of Tamil Nadu. Indian Veterinary Journal 94(9): 68–70.

Pappas G. 2010. The changing Brucella ecology: Novel reservoirs, new threats. International Journal of Antimicrobial Agents 36(11): S8–S11. DOI: https://doi.org/10.1016/j.ijantimicag.2010.06.013

Polding J B. 1942. Brucellosis in India. The Indian Journal of Veterinary Science 13: 27–34.

Priyanka S B N, Patel K B, Chauhan H, Chandel B S and Kashyap S K. 2018. Molecular epidemiology of Brucella abortus among buffaloes in Western Rajasthan. Journal of Animal Research 8(8):1065–69. DOI: https://doi.org/10.30954/2277-940X.12.2018.19

Pushpa R N and Kumari B P. 2005. Serosurveillance of brucellosis in bovine and ovine. Indian Veterinary Journal 82(6): 672–73.

Radostits O M, Gay C C, Blood D C and Hinchcliff K W. 2000. Veterinary Medicine: A Textbook of the Diseases of Cattle, Sheep, Pigs, Goats and Horses. 9th edn, pp.1877. (Ed) Saunders W B. London.

Renukaradhya G J, Isloor S and Rajasekhar M. 2002. Epidemiology, zoonotic aspects, vaccination and control/ eradication of brucellosis in India. Veterinary Microbiology 90(1-4): 183–95.Sargent C, Lastella M, Romyn G, Versey N, Miller D J and Roach G D. 2018. How well does a commercially available wearable device measure sleep in young athletes? Chronobiology International 35(6): 754–58. DOI: https://doi.org/10.1080/07420528.2018.1466800

Seventer V J M and Hochberg N S. 2017. Principles of infectious diseases: Transmission, diagnosis, prevention, and control. International Encyclopedia of Public Health 6(2): 22–39. DOI: https://doi.org/10.1016/B978-0-12-803678-5.00516-6

Shome R, Padmashree B S, Krithiga N, Triveni K, Sahay S, Shome B R, Singh P and Rahman H. 2014. Bovine brucellosis in organized farms of India - An assessment of diagnostic assays and risk factors. Advances in Animal and Veterinary Sciences 2(10): 557–64. DOI: https://doi.org/10.14737/journal.aavs/2014/2.10.557.564

Shome R, Kalleshamurthy T, Rathore Y, Ramanjinappa K D, Skariah S, Nagaraj C, Mohandoss N, Sahay S, Shome B R, Kuralayanapalya P S, Roy P and Hemadri D. 2021. Spatial sero-prevalence of brucellosis in small ruminants of India: Nationwide cross-sectional study for the year 2017-2018. Transboundary Emerging Diseases 68(4): 2199–208. DOI: https://doi.org/10.1111/tbed.13871

Shome R, Triveni K, Swati S, Ranjitha S, Krithiga N, Shome B R, Nagalingam M, Rahman H and Barbuddhe S B. 2019. Spatial seroprevalence of bovine brucellosis in India-A large random sampling survey. Comparative Immunology, Microbiology and Infectious Diseases 65(5): 124–27. DOI: https://doi.org/10.1016/j.cimid.2019.05.002

Smits H L and Kadri S M. 2005. Brucellosis in India: A deceptive infectious disease. The Indian Journal of Medical Research 122(5): 375–84.

Thiruvenkadan A K, Ramanujam R and Dharan M. 2013. Buffalo genetic resources of India and their conservation. Buffalo Bulletin 32(1): 227–35.

Trangadia B J and Patel R M. 2016. Sero-prevalence of brucellosis in buffaloes in Gujarat: An on-farm case study. Buffalo Bulletin 35(1): 121–24.

Verma S, Jatav G, Shukla S, Jayraw A, Chauhan H and Shrivastava N. 2019. Seroprevalence of brucellosis in buffaloes of Malwa region of Madhya Pradesh. International Journal of Livestock Research 9(3): 256–62. DOI: https://doi.org/10.5455/ijlr.20181114062927

Walker R L. 1999. Brucella in Veterinary Microbiology. pp. 196-203. (Eds.) Dwight C H and Yuang C Z. Blackwell Science, Cambridge, Mass, USA.