Effect of lyophilized heterologous seminal plasma supplementation on Marwari stallion epididymal spermatozoa during 24 hours cool storage

VACHNA  RAM; DINESH  JHAMB; MITESH  GAUR; NAVEEN  KUMAR; THIRUMALA RAO  TALLURI; OMPRAKASH  SOLANKI; ROHIT  JUNEJA; KALPESH KUMAR  PARGI; DEVKI  NANDAN; SHARAD  ARORA; HIMANSHU  VYAS

doi:10.56093/ijans.v94i9.145538

Authors

VACHNA RAM Veterinary Hospital, Pali, Rajasthan
DINESH JHAMB Veterinary Hospital, Pali, Rajasthan
MITESH GAUR Department of Veterinary Gynaecology and Obstetrics, CVAS, Navania, Vallabhnagar, Udaipur, Rajasthan
NAVEEN KUMAR Department of Veterinary Gynaecology and Obstetrics, CVAS, Navania, Vallabhnagar, Udaipur, Rajasthan
THIRUMALA RAO TALLURI Equine Production Campus, ICAR-National Research Centre on Equines, Bikaner, Rajasthan
OMPRAKASH SOLANKI Department of Veterinary Gynaecology and Obstetrics, CVAS, Navania, Vallabhnagar, Udaipur, Rajasthan
ROHIT JUNEJA Department of Veterinary Gynaecology and Obstetrics, CVAS, Navania, Vallabhnagar, Udaipur, Rajasthan
KALPESH KUMAR PARGI Department of Veterinary Gynaecology and Obstetrics, CVAS, Navania, Vallabhnagar, Udaipur, Rajasthan
DEVKI NANDAN Department of Veterinary Gynaecology and Obstetrics, CVAS, Navania, Vallabhnagar, Udaipur, Rajasthan
SHARAD ARORA Veterinary Polyclinic, Udaipur, Rajasthan
HIMANSHU VYAS Veterinary Polyclinic, Udaipur, Rajasthan

https://doi.org/10.56093/ijans.v94i9.145538

Keywords:

Castration, Cool sperm, Equine, Epididymal sperm, HOST, Seminal plasma

Abstract

A study was carried out to investigate the effect of storage of testes-epididymis complex at 5^oC for 24 h and supplementation of lyophilized heterologous seminal plasma (LHSP) at different concentrations on the sperm quality during cool storage. Six pairs of testicles were collected immediately after orchiectomy from six stallions. One testes-epididymis (F1) was immediately processed for sperm retrieval and second testes-epididymis (F2) complex of each stallion was stored at 5℃ for 24 h. Spermatozoa were harvested from the epididymis by flotation technique and evaluated. Highly significant difference was observed among mean values of progressive motility (%) of epididymal sperm after 0 and 24 h of testicular-epididymal complex storage at 5°C, while no significant difference was observed among mean values of live sperm, membrane integrity and acrosomal integrity (%). Both epididymal sperm suspensions were supplemented with skim milk glucose semen extender fortified with different concentrations (0, 2, 5 and 10 mg/ml) of LHSP and cooled for 24 h. Sperm suspension evaluation at 0, 12 and 24 h of cool storage revealed that addition of 2 mg/ml LHSP has beneficial effect as compared to control on progressive motility and HOST in both epididymal sperm suspension at 12 h and 24 h of cool storage at 5℃. From the current study, it was inferred that the sperm quality was not deteriorated even after the sperm retrieval after 24 h from the epididymis on storage at 5℃. Further, LHSP supplemented @2 mg/ml originated from good fertility stallion is the suitable concentration for cool-storage of equine epididymal sperm suspension.

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