Effect of feeding rice bran crude lecithin containing total mixed ration on rumen fermentation and blood biochemical profile in Vrindavani crossbred cattle
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Keywords:
Blood metabolites, Crossbred, Microbial enzymes, Rice bran crude lecithin, Rumen fermentationAbstract
The effects of dietary supplementation with rice bran crude lecithin (RBCL) on rumen fermentation and blood biochemical parameters in crossbred cattle were investigated in the present study. For the study, 18 male crossbred Vrindavani cattle (BW 315.66±19.95 kg and 30-36 months of age) were selected and randomly divided into three groups, namely, RBCL-0, RBCL-4, and RBCL-8, containing six animals per group based on a randomized block design (RBD) and offered a total mixed ration (TMR) supplemented with 0, 4, and 8% RBCL in their concentrate mixture, respectively. The rumen pH was unaffected by RBCL supplementation, whereas the ammonia nitrogen (NH3-N) concentration was increased in the RBCL-supplemented groups. The molar concentrations of acetate, butyrate, and TVFA were unaffected, whereas the propionate concentration increased and the A/P ratio decreased in the RBCL-supplemented groups. The activity of rumen microbial enzymes (carboxy methyl cellulase, xylanase, and avicelase) was not influenced by RBCL supplementation. The inclusion of RBCL in crossbred cattle diet did not significantly alter Hb, haematocrit, total protein, albumin, globulin, A/G ratio, BUN, ALT, AST, and total cholesterol. It can be concluded that supplementation of RBCL by replacing an equal amount of corn did not show any adverse effect on rumen fermentation and blood-biochemical profile.
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References
Adhikari S and Adhikari J. 1986. Indian ricebran lecithin. Journal of the American Oil Chemists’ Society 63: 1367–69. DOI: https://doi.org/10.1007/BF02679604
Agarwal N, Kamra D N, Chaudhary L C, Agarwal I, Sahoo A and Pathak N N. 2002. Microbial status and rumen enzyme profile of crossbred calves fed on different microbial feed additives. Letters in Applied Microbiology 34(5): 329–36. DOI: https://doi.org/10.1046/j.1472-765X.2002.01092.x
AOAC. 2000. Official Methods of Analysis, 17th ed. AOAC International: Arlington, VA, USA.
Chen G J, Zhang R, Wu J H, Shang Y S, Li X D, Qiong M, Wang P C, Li S G, Gao Y H and Xiong X Q. 2020. Effects of soybean lecithin supplementation on growth performance, serum metabolites, ruminal fermentation and microbial flora of beef steers. Livestock Science 240: 104121. DOI: https://doi.org/10.1016/j.livsci.2020.104121
Cho S, Kim D H, Hwang I H and Choi N J. 2013. Investigation of dietary lysophospholipid (Lipidol TM) to improve nutrients availability of diet with in vitro rumen microbial fermentation test. Journal of the Korean Society of Grassland and Forage Science 33(3): 206–12. DOI: https://doi.org/10.5333/KGFS.2013.33.3.206
Cottyn B G and Boucque C V. 1968. Rapid method for the gas- chromatographic determination of volatile fatty acids in rumen fluid. Journal of Agricultural and Food Chemistry 16(1): 105–07. DOI: https://doi.org/10.1021/jf60155a002
Garton G A, Lough A K and Vioque E. 1961. Glyceride hydrolysis and glycerol fermentation by sheep rumen contents. Microbiology 25(2): 215–25. DOI: https://doi.org/10.1099/00221287-25-2-215
Huo Q, Li B, Cheng L, Wu T, You P, Shen S, Li Y, He Y, Tian W, Li R and Li C. 2019. Dietary supplementation of lysophospholipids affects feed digestion in lambs. Animals 9(10): 805. DOI: https://doi.org/10.3390/ani9100805
Hwang I H, Lee C H, Kim S W, Sung H G, Lee S Y, Lee S S, Hong H, Kwak Y C and Ha J K. 2008. Effects of mixtures of Tween80 and cellulolytic enzymes on nutrient digestion and cellulolytic bacterial adhesion. Asian-Australasian Journal of Animal Sciences 21(11): 1604–09. DOI: https://doi.org/10.5713/ajas.2008.80333
ICAR. 2013. Nutrient’s Requirements of Cattle and Buffalo. Indian Council of Agricultural Research, New Delhi.
Immig I, Wirth S J, Wolf G A and Abel H. 1991. Quantifizierung der cellulaseaktivität und nachweis von fettsäure‐coating‐ effekten im pansen von schafen. Journal of Animal Physiology and Animal Nutrition 66: 45–52. DOI: https://doi.org/10.1111/j.1439-0396.1991.tb00276.x
Jenkins T C. 1990. Nutrient digestion, ruminal fermentation, and plasma lipids in steers fed combinations of hydrogenated fat and lecithin. Journal of Dairy Science 73: 2934–39. DOI: https://doi.org/10.3168/jds.S0022-0302(90)78982-5
Jenkins T C. 1993. Lipid metabolism in the rumen. Journal of Dairy Science 76: 3851–63. DOI: https://doi.org/10.3168/jds.S0022-0302(93)77727-9
Jenkins T C and Fotouhi N. 1990. Effects of lecithin and corn oil on site of digestion, ruminal fermentation and microbial protein synthesis in sheep. Journal of Animal Science 68: 460–66. DOI: https://doi.org/10.2527/1990.682460x
Jenkins T C, Gimenez T and Cross D L. 1989. Influence of phospholipids on ruminal fermentation in vitro and on nutrient digestion and serum lipids in sheep. Journal of Animal Science 67: 529–37. DOI: https://doi.org/10.2527/jas1989.672529x
Kim H, Kim B, Cho S, Kwon I and Seo J. 2020. Dietary lysophospholipids supplementation inhibited the activity of lipolytic bacteria in forage with high oil diet: An in-vitro study. Asian-Australasian Journal of Animal Science 33: 1590. DOI: https://doi.org/10.5713/ajas.19.0850
Lee C, Morris D L, Copelin J E, Hettick J M and Kwon I H. 2019. Effects of lysophospholipids on short-term production, nitrogen utilization, and rumen fermentation and bacterial population in lactating dairy cows. Journal of Dairy Science 102(4): 3110–20. DOI: https://doi.org/10.3168/jds.2018-15777
Lee S S, Ahn B H, Kim H S, Kim C H, Cheng K J and Ha J K. 2003. Effects of nonionic surfactants on enzyme distributions of rumen contents, anaerobic growth of rumen microbes, rumen fermentation characteristics and performances of lactating cows. Asian-Australasian Journal of Animal Sciences 16(1): 104–15. DOI: https://doi.org/10.5713/ajas.2003.104
Li X Z, Park B K, Hong B C, Ahn J S and Shin J S. 2017. Effect of soy lecithin on total cholesterol content, fatty acid composition and carcass characteristics in the Longissimus dorsi of Hanwoo steers (Korean native cattle). Animal Science Journal 88(6): 847–53. DOI: https://doi.org/10.1111/asj.12660
Liu H, Liu T, Fan H, Gou M, Li G, Ren H, Wang D and Cheng Z. 2018. Corn lecithin for injection from deoiled corn germ: Extraction, composition, and emulsifying properties. European Journal of Lipid Science and Technology 120(3): 1700288. DOI: https://doi.org/10.1002/ejlt.201700288
Livestock Census. 2019. Department of Animal Husbandry and Dairying, Ministry of Agriculture, Government of India, New Delhi.
Maia M R, Chaudhary L C, Figueres L and Wallace R J. 2007. Metabolism of polyunsaturated fatty acids and their toxicity to the microflora of the rumen. Antonie Van Leeuwenhoek 91: 303–14. DOI: https://doi.org/10.1007/s10482-006-9118-2
Maia M R, Chaudhary L C, Bestwick C S, Richardson A J, McKain N, Larson T R, Graham I A and Wallace R J. 2010. Toxicity of unsaturated fatty acids to the biohydrogenating ruminal bacterium, Butyrivibrio fibrisolvens. BMC Microbiology 10: 1–10. DOI: https://doi.org/10.1186/1471-2180-10-52
Miller G L. 1959. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analytical Chemistry 31(3): 426–28. DOI: https://doi.org/10.1021/ac60147a030
Movagharnezhad M, Chashnidel Y, Teimouri Yansari A and Gholizadeh M. 2023. The effects of lysophospholipid on performance, ruminal bacteria and some blood parameters in lactating holstein dairy cows. Iranian Journal of Applied Animal Science 13(3): 457–65.
Paul B N, Srivastava A, Walli T K, Prasad T and Sharma D D. 2000. In vivo rumen fermentation as affected by type of dietary lipids. Indian Journal of Animal Nutrition 17(2): 132–36.
Ravi Kiran G, Suresh K P, Sampath K T, Giridhar K and Anandan S. 2012. Modeling and Forecasting Livestock and Fish Feed Resources: Requirements and Availability in India. National Institute of Animal Nutrition and Physiology, Bengaluru.
Reddy J R C, Rao B V S K, Chakrabarti P P, Karuna M S L, Prabhavathi Devi B L A and Prasad R B N. 2008. Characterization of rice bran lyso-lecithin and evaluation of its polar lipids for surfactant properties. Lipid Science and Technology 40: 10–15.
Reddy P B, Chaturvedi V B, Chaudhary L C, Kala A and Thamizhan P. 2023. Effect of rice bran crude lecithin supplementation on feed intake, nutrient utilization, and methane emission in crossbred cattle. Indian Journal of Animal Nutrition 40(3): 313–22. DOI: https://doi.org/10.5958/2231-6744.2023.00039.7
Reis M E, Toledo A F D, da Silva A P, Poczynek M, Fioruci E A, Cantor M C, Greco L and Bittar C M M. 2021. Supplementation of lysolecithin in milk replacer for Holstein dairy calves: Effects on growth performance, health, and metabolites. Journal of Dairy Science 104(5): 5457–66. DOI: https://doi.org/10.3168/jds.2020-19406
Sontakke U B, Kaur H, Tyagi A K, Kumar M and Hossain S A. 2014. Effect of feeding rice bran lyso-phospholipids and rumen protected fat on feed intake, nutrient utilization and milk yield in crossbred cows. The Indian Journal Animal Sciences 84: 998–1003. DOI: https://doi.org/10.56093/ijans.v84i9.43680
Talapatra S K, Ray S C and Sen K C. 1940. The analysis of mineral constituents in biological materials. 1. Estimation of phosphorus, chlorine, calcium, magnesium, sodium and potassium in food-stuffs. Indian Journal of Veterinary Science 10: 243–58.
Tewari D, Chaturvedi V B and Dutta N. 2022a. Lactation performance of cows fed ration containing corn substituted rice bran crude lecithin. Animal Nutrition and Feed Technology 22(3): 619–26. DOI: https://doi.org/10.5958/0974-181X.2022.00049.X
Tewari D, Chaturvedi V B, Chaudhary L C, Verma A K and Chaudhary S K. 2022b. Effect of dietary supplementation of rice bran crude lecithin on nutrient metabolism, methanogenesis and metabolic profile of crossbred calves. The Indian Journal of Animal Sciences 92(5): 585–91. DOI: https://doi.org/10.56093/ijans.v92i5.124407
Tewari D, Chaturvedi V B, Dutta N and Chaudhary S K. 2023. Effect of rice bran crude lecithin blended diet on rumen ecology, metabolic profile, and milk fat indices affecting human health. European Journal of Lipid Science and Technology 125(8): e2200150. DOI: https://doi.org/10.1002/ejlt.202200150
Ullah A, Sarwar I, Suheryani I, Ahmad S, Andlib S, Buzdar J A, Kakar M U and Arain M A. 2024. Role of dietary lecithin as an emulsifying agent in poultry nutrition: Efficacy and feasibility. World’s Poultry Science Journal 80(1): 187–206. DOI: https://doi.org/10.1080/00439339.2023.2268584
Vandana V, Karuna M S L, Reddy J R C, Vijeeta T, Rao B V S K, Prabhavathi D B L A and Prasad R B N. 2003. Bleaching and enrichment of phospholipid content in commercial rice bran lecithin. Journal of Technologists Association of India 35: 145–50.
Van Soest P V, Robertson J B and Lewis B A. 1991. Methods for dietary fibre, neutral detergent fibre, and nonstarch polysaccharides in relation to animal nutrition. Journal of Dairy Science 74(10): 3583–97. DOI: https://doi.org/10.3168/jds.S0022-0302(91)78551-2
Wang Y, McAllister T A, Baah J, Wilde R, Beauchemin K A, Rode L M, Shelford J A, Kamande G M and Cheng K J. 2003. Effects of Tween 80 on in vitro fermentation of silages and interactive effects of Tween 80, monensin and exogenous fibrolytic enzymes on growth performance by feedlot cattle. Asian-Australasian Journal of Animal Sciences 16(7): 968–78. DOI: https://doi.org/10.5713/ajas.2003.968
Weatherburn M W. 1967. Phenol-hypochlorite reaction for determination of ammonia. Analytical Chemistry 39(8): 971–74. DOI: https://doi.org/10.1021/ac60252a045
Wettstein H R, Machmüller A and Kreuzer M. 2000. Effects of raw and modified canola lecithins compared to canola oil, canola seed and soy lecithin on ruminal fermentation measured with rumen simulation technique. Animal Feed Science Technology 85: 153–69. DOI: https://doi.org/10.1016/S0377-8401(00)00149-8
Zeleny J and Kroupova A. 1988. Productive and metabolic verification of lecithin as component of diets for fattening bulls. Veterinarstvi 38: 168.
Zhang M, Bai H, Zhao Y, Wang R, Li G, Zhang G and Zhang Y. 2022a. Effects of dietary lysophospholipid inclusion on the growth performance, nutrient digestibility, nitrogen utilization, and blood metabolites of finishing beef cattle. Antioxidants 11(8): 1486. DOI: https://doi.org/10.3390/antiox11081486
Zhang M, Bai H, Zhao Y, Wang R, Li G, Zhang Y and Jiao P. 2022b. Effects of supplementation with lysophospholipids on performance, nutrient digestibility, and bacterial communities of beef cattle. Frontiers in Veterinary Science 9: 927369. DOI: https://doi.org/10.3389/fvets.2022.927369
Zinn R A. 1989. Influence of level and source of dietary fat on its comparative feeding value in finishing diets for feedlot steers: Metabolism. Journal of Animal Science 67(4): 1038–49. DOI: https://doi.org/10.2527/jas1989.6741038x
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