Impact of varying doses of Moringa leaf extract supplementation in the cryopreservation media on sperm quality, antioxidant capacity and antimicrobial activity of frozen-thawed buck spermatozoa

CHETNA  GANGWAR; ASHOK  KUMAR; K  GURURAJ; A K  MISHRA; RAVI  RANJAN; MANISH  KUMAR; ALOK  RAI; NIKITA  MITTAL

doi:10.56093/ijans.v94i4.146821

Authors

CHETNA GANGWAR ICAR-Central Institute for Research on Goats, Farah, Mathura, Uttar Pradesh 281 122 India
ASHOK KUMAR ICAR-Central Institute for Research on Goats, Farah, Mathura, Uttar Pradesh 281 122 India
K GURURAJ ICAR-Central Institute for Research on Goats, Farah, Mathura, Uttar Pradesh 281 122 India
A K MISHRA ICAR-Central Institute for Research on Goats, Farah, Mathura, Uttar Pradesh 281 122 India
RAVI RANJAN ICAR-Central Institute for Research on Goats, Farah, Mathura, Uttar Pradesh 281 122 India
MANISH KUMAR ICAR-Central Institute for Research on Goats, Farah, Mathura, Uttar Pradesh 281 122 India
ALOK RAI Animal Husbandry and Dairying, GLA University, Mathura, Uttar Pradesh
NIKITA MITTAL ICAR-Central Institute for Research on Goats, Farah, Mathura, Uttar Pradesh 281 122 India

https://doi.org/10.56093/ijans.v94i4.146821

Keywords:

Antimicrobial potential, Antioxidant, Buck semen, Fertility, Moringa oleifera

Abstract

The current investigation was planned to evaluate the effect of Moringa leaves also called Moringa oleifera (M. oleifera) aqueous extract on buck semen quality during cryopreservation, and its antimicrobial potential against the Escherichia coli (E. coli) isolated from the semen samples. Semen was collected from 8 Jakhrana bucks, and from each buck, 8 ejaculates were collected. Semen samples were subjected to bacteriological studies and pathogenic coli were isolated from semen samples. Then, various concentrations of M. oleifera extract were evaluated for its antimicrobial potential. Good quality semen samples were pooled during each collection. Pooled semen samples were then divided into 4 equal parts, and diluted in TRIS buffer containing different concentration of M. oleifera leaf aqueous extract (Different groups, i.e. T₁-50 mg, T₂-100 mg, T₃-200 mg, C-0 mg of M. oleifera aqueous extract in 100 ml TRIS Buffer). Semen samples were evaluated for various sperm characteristics after cryopreservation. oleifera aqueous extract supplemented groups showed significant enhancement in sperm viability, sperm motility, acrosomal integrity and plasma membrane integrity. The treatment significantly reduced the lipid peroxidation in supplemented groups and M. oleifera extract shows the potent antimicrobial activity against the E. coli isolated from buck semen.

Downloads

Download data is not yet available.

References

Abalaka M E, Daniyan S Y, Oyeleke S B and Adeyemo S O. 2012. The antibacterial evaluation of Moringa oleifera leaf extracts on selected bacterial pathogens. Journal of Microbiology Research 2(2): 1–4.

Allai L, Druart X, Öztürk M, BenMoula A, Nasser B and El Amiri B. 2016. Protective effects of Opuntia ficus-indica extract on ram sperm quality, lipid peroxidation and DNA fragmentation during liquid storage. Animal Reproduction Science 1(175): 1–9.

Bajia N P, Burdak S, Yadav S P and Kumar A. 2022. Effect of giloy (Tinospora cordifolia) ethanolic extract supplementation to ram semen extender on sperm abnormality in chilled semen. Journal of Pharmaceutical Innovation (7): 3361–5.

Carrera-Chávez J M, Jiménez-Aguilar E E, Acosta-Pérez T P, Núñez-Gastélum J A, Quezada-Casasola A, Escárcega-Ávila A M, Itza-Ortiz M F and Orozco-Lucero E. 2020. Effect of Moringa oleifera seed extract on antioxidant activity and sperm characteristics in cryopreserved ram semen. Journal of Applied Animal Research 48(1): 114–20.

Dapawole R R and Sirappa I P. 2021. Effect concentration of moringa (Moringa oleifera Lam) leaf extract in citrate-egg yolk in maintaining motility and viability of spermatozoa of Kacang goat. Jurnal Sain Peternakan Indonesia 16(4): 340–6.

El-Seadawy I E, Kotp M S, El-Maaty A M, Fadl A M, El-Sherbiny H R and Abdelnaby E A. 2022. The impact of varying doses of moringa leaf methanolic extract supplementation in the cryopreservation media on sperm quality, oxidants, and antioxidant capacity of frozen-thawed ram sperm. Tropical Animal Health and Production 54(6): 344.

El-Sheshtawy R I and El-Nattat W S. 2020. Effect of addition of Moringa oleifera extract to tris extender on the preservability of cattle bull semen. International Journal of Veterinary Science 9(3): 417–20.

Enwuru C A, Iwalokun B, Enwuru N V and Ezechi O. 2020. Infertile semen bacterial isolates and their local multiple antibacterial resistance pattern in Lagos, Nigeria. Journal of Clinical and Diagnostic Research 14(7).

Forson A O, Tsidi W B, Nana-Adjei D, Quarchie M N and Obeng-Nkrumah N. 2018. Escherichia coli bacteriuria in pregnant women in Ghana: Antibiotic resistance patterns and virulence factors. BMC Research Notes 11(1): 1–7.

Gangwar C, Kharche S D, Mishra A K, Saraswat S, Kumar N and Sikarwar A K. 2020. Effect of diluent sugars on capacitation status and acrosome reaction of spermatozoa in buck semen at refrigerated temperature. Tropical Animal Health and Production 3409–15.

Gangwar C, Kumar R, Kharche S D, Jindal S K, Chaudhary U B and Mishra A K. 2019. Effect of azolla supplementation in feed on semen freezability in bucks. Indian Journal of Animal Sciences 89(4): 398–401.

Gangwar C, Kumaresan G, Mishra A K, Kumar A, Pourouchottamane R and Rai B. 2023. Herbal remedies for male infertility and spermatogenic activity in animals: A review. Indian Journal of Animal Sciences 93: 843–52.

Gangwar C, Mishra A K, Gururaj K, Kumar A, Kharche S D, Saraswat S, Kumar R and Ramachandran N. 2021. Semen quality and total microbial load: An association study in important Indian Goat breeds during different seasons. Andrologia 53(4): e13995.

Gangwar C, Ranjan R, Satish K, Kharche S D, Goel A K, Ramachandran N and Jindal S K. 2014. Use of chelating agent for optimum post thaw quality of buck semen. Indian Journal of Animal Sciences 84(8): 839–41.

Guedea Betancourt J J, Quezada Casasola A, Núñez Gastélum J A, Orozco Lucero E, Escárcega Ávila A M, Soler Valls A J and Carrera Chávez J M. 2022. Effect of Moringa oleifera seed extract on antimicrobial activity and in vitro fertilization ability of cryopreserved ram semen. Reproduction in Domestic Animals 57(12): 1564–571.

Gunzburg S T, Tornieporth N G and Riley L W. 1995. Identification of enteropathogenic Escherichia coli by PCR- based detection of the bundle-forming pilus gene. Journal of Clinical Microbiology 33(5): 1375–377.

Hammad M E, Wafa W M, Gabr A A and Elkishky A Y. 2019. Different types and levels of Moringa oleifera leaf extract as a source of antibiotics in Friesian bull semen extender. Journal of Animal and Poultry Production 10(3): 67–71.

Hussein H S. 2007. Prevalence and pathogenicity of Shiga toxin- producing Escherichia coli in beef cattle and their products. Journal of Animal Science 85(suppl_13): E63–72.

Jabbar A D. 2013. Phenotypic and genotypic detection of extended-spectrum β-Lactamases (ESBL) among Escherichia coli isolated from symptomatic female’s genital tract infection. Journal of Wasit for Science and Medicine 6(1): 1–8.

Jayawardana B C, Liyanage R, Lalantha N, Iddamalgoda S and Weththasinghe P. 2015. Antioxidant and antimicrobial activity of drumstick (Moringa oleifera) leaves in herbal chicken sausages. LWT-Food Science and Technology 64(2): 1204–208.

Kalaiyan G, Suresh S, Prabu K M, Thambidurai S, Kandasamy M, Pugazhenthiran N, Kumar S K and Muneeswaran T. 2021. Bactericidal activity of Moringa oleifera leaf extract assisted green synthesis of hierarchical copper oxide microspheres against pathogenic bacterial strains. Journal of Environmental Chemical Engineering 9(1): 104847.

Kumar A, Mishra A K, Kharche S D, Swaroop K, Pourouchttmane R, Goel R and Singh S. 2022. Asparagus racemosus aqueous extract improves seminal antioxidant status and sperm characteristics in buck semen at refrigeration temperature. Research Square (Pre-print)

Kumar A, Singh S, Mahour K, Vihan V S and Gururaj K. 2011. Phytochemical analysis of some indigenous plants potent against ectoparasite. Asian Journal of Experimental Biological Sciences 2(3): 506–9.

Kumaresan G, Gangwar C, Mishra A K, Kumar A, Kharche S D, Singh N P and Pachoori A. 2022. Occurrence, molecular characterization and antimicrobial-resistance pattern of Staphylococcus species isolates from buck semen. Archives of Microbiology 204(2): 135.

López-Saucedo C, Cerna J F, Villegas-Sepulveda N, Thompson R, Velazquez F R, Torres J, Tarr P I and Estrada-Garcia T. 2003. Single multiplex polymerase chain reaction to detect diverse loci associated with diarrheagenic Escherichia coli. Emerging infectious diseases 9(1): 127.

Mezhoud H, Boyen F, Touazi L H, Garmyn A, Moula N, Smet A, Haesbrouck F, Martel A, Iguer-Ouada M and Touati A. 2015. Extended spectrum β-lactamase producing Escherichia coli in broiler breeding roosters: Presence in the reproductive tract and effect on sperm motility. Animal Reproduction Science 159: 205–11.

Modak R, Das Mitra S, Krishnamoorthy P, Bhat A, Banerjee A, Gowsica B R, Bhuvana M, Dhanikachalam V, Natesan K, Shome R and Shome B R. 2012. Histone H3K14 and H4K8 hyperacetylation is associated with Escherichia coli-induced mastitis in mice. Epigenetics 7(5): 492–501.

Moyo B, Oyedemi S, Masika P J and Muchenje V. 2012. Polyphenolic content and antioxidant properties of Moringa oleifera leaf extracts and enzymatic activity of liver from goats supplemented with Moringa oleifera leaves/sunflower seed cake. Meat Science 91(4): 441–7.

Ojiako E N. 2014. Phytochemical analysis and antimicrobial screening of Moringa oleifera leaves extract. The International Journal of Engineering and Science 3(3): 32–5.

Paton A W and Paton J C. 1998. Detection and characterization of Shiga toxigenic Escherichia coli by using multiplex PCR assays for stx 1, stx 2, eaeA, enterohemorrhagic E. coli hlyA, rfb O111, and rfb O157. Journal of Clinical Microbiology 36(2): 598–602.

Ray R and Singh P. 2022. Prevalence and implications of shiga toxin-producing E. coli in farm and wild ruminants. Pathogens 11(11): 1332.

Rusz A, Pilatz A, Wagenlehner F, Linn T, Diemer T, Schuppe H C, Lohmeyer J, Hossain H and Weidner W. 2012. Influence of urogenital infections and inflammation on semen quality and male fertility. World Journal of Urology 30: 23–30.

Sokunbi O, Ajani O, Lawanson A and Amao E. 2015. Antibiotic potential of Moringa leaf (Moringa oleifera Lam.) crude extract in bull semen extender. European Journal of Medicinal Plants 9(2): 1–8.

Stacy-Phipps S, Mecca J J, Weiss J B. 1995. Multiplex PCR assay and simple preparation method for stool specimens detect enterotoxigenic Escherichia coli DNA during course of infection. Journal of Clinical Microbiology 33(5): 1054–59.

Susilowati S, Sardjito T, Mustofa I, Widodo O S and Kurnijasanti R. 2021. Effect of green tea extract in extender of Simmental bull semen on pregnancy rate of recipients. Animal Bioscience 34(2): 198.

Swidan K, E L Sherbiny G, Mansour A, El-Haw M, Askar A and El-Badry M. 2020. Antibacterial evaluation of Punica granatum as therapeutic agents against multi drug resistance bacteria isolated from infertile male’s semen. Al-Azhar International Medical Journal 1(5): 125–32.

Wahjuningsih S, Ciptadi G, Ihsan M N, Isnaini N and Rahayu S. 2019. Supplementation of Moringa oleifera leaves’ extract in Tris-egg yolk extender on the quality and fertility of cryopreserved Senduro goat sperm. Livestock Research for Rural Development 31(12):1–9.

Wen F, Li Y, Feng T, Du Y, Ren F, Zhang L, Han N, Ma S, Li F, Wang P and Hu J. 2019. Grape seed procyanidin extract (GSPE) improves goat sperm quality when preserved at 4°C. Animals 9(10): 810.