Genotypic profiling of A1 and A2 β-casein alleles in Punganur cattle using PCR-RFLP

AVINASH  P; ASWANI KUMAR  K; PUNYA KUMARI  B

doi:10.56093/ijans.v94i8.148106

Authors

AVINASH P School of Veterinary and Animal Sciences, Centurion University of Technology and Management, Paralakhemundi, Gajapati, Odisha
ASWANI KUMAR K N.T.R. College of Veterinary Science, Sri Venkateswara Veterinary University, Gannavaram, Andhra Pradesh
PUNYA KUMARI B 3College of Veterinary Science, Sri Venkateswara Veterinary University, Tirupati, Andhra Pradesh

https://doi.org/10.56093/ijans.v94i8.148106

Keywords:

Allelic frequency, A1 and A2 alleles, β-casein gene, Dde I, Genetic frequency, Punganur cattle, PCR-RFLP

Abstract

Milk is regarded as nature’s perfect food due to the presence of essential nutrients required for the growth and development of mammals. The consumption of milk with a specific β casein variant either A1 or A2 had a relationship with the risk of diseases has been identified. Considering the importance of β casein variants with the health significance, the present study was undertaken in Punganur cattle (N=175) by using PCR-RFLP with Dde I restriction enzyme that showed the distribution of A1A2 (121 bp, 86 bp, and 35 bp) in 5 animals, A2A2 (86 bp and 35 bp) in 170 animals with no evidence of A1A1 genotype. The genotypic frequency of the A1A2 heterozygous β-casein variant of exon7 of the CSN2 gene was 0.029, whereas, for the A2A2 homozygous β-casein variant was 0.971 and the A1A1 homozygous β-casein variant is absent in Punganur cattle. The A1 allelic frequency of exon7 of the CSN2 gene was 0.014, whereas, the A2 gene frequency is 0.986 in Punganur cattle. The study indicates that there is a minimum genetic variability in the tested population of the Punganur cattle breed suggesting less or lower level of crossbreeding activities with exotic animals.

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References

Boltstein D R L, White M, Skolnik R W and Davis. 1980. Construction of a genetic linkage map in man using restriction fragment length polymorphism. The American Journal of Human Genetics 32: 314–31.

Crow J F and Kimura M. 1970. An Introduction to Population Genetics Theory. Burgess Publishing, Minneapolis.

Eigel W N, Butler J E, Ernstrom C A, Farrell H M, Halwarker V R, Jenness R and Whitney R M. 1984. Nomenclature of proteins of cow’s milk: Fifth revision. Journal of Dairy Science 67: 1599–631.

Elliot R B, Harris D P, HilL J P, Bibby N J and Wasmuth H E. 1999. Type I (insulin dependent) diabetes mellitus and cow milk: Casein variant consumption. Diabetologia 42: 292–96.

Farrel H M, Jimenez-Flores R, Bleck G T, Brown E M, Butler J E and Creamer L K. 2004. Nomenclature of the proteins of cows’ milk—sixth revision. Journal of Dairy Science 87(6):1641–674.

Hanusova E, Huba J, Oravcova M, Polak P and Vrtkova I. 2010. Genetic variants of β-casein in Holstein dairy cattle in Slovakia. Slovak Journal of Animal Science 43(2): 63–66.

Kamiñski S, Ruoea A and Cieoeliñska A. 2006. A note on frequency of A1 and A2 variants of bovine beta-casein locus in Polish Holstein bulls. Journal of Animal and Feed Sciences 15: 195–98.

Kumar S, Singh R V and Chauhan A. 2018. Molecular characterization of A1/A2 beta–casein alleles in Vrindavani crossbred and Sahiwal cattle. Indian Journal of Animal Research 53(2): 151–55.

Laugesen M and Elliott R. 2003. Ischaemic heart disease, Type 1 diabetes, and cow milk A1 beta-casein. New Zealand Medical Journal 116 (1168): U295.

Malarmathi M, Senthil Kumar T M A, Parthiban M, Muthuramalingam T and Palanisammi A. 2014. Analysis of â- casein gene of A1 and A2 genotype using allele specific PCR in Kangeyam and HF crossbred cattle in Tamil Nadu. Indian Journal of Veterinary and Animal Sciences Research 43: 310–15.

McLachlan C N. 2001. β-casomorphins and related peptides: Recent developments. Peptides 22(6): 995–1005.

Mclachlan C N S. 2001. Beta-casein ischemic heart diseases mortality and other illnesses. Medical Hypotheses 56: 262–72.

Miluchova M, Vasicek D, Bauerova M and Gabor M. 2014. β-casein gene polymorphism in Slovak spotted cattle by PCR-RFLP method. Animal Biotechnology Genetic Resources 57: 35–40.

Mishra B P, Manishi M, Prakash B, Monika S, Kapila R, Kishore A and Kataria R R. 2009. Status of milk protein, β -casein variants among Indian milch animals. Indian Journal of Animal Sciences 79(7): 722–25.

Nei M. 1973. Analysis of gene diversity in subdivided populations. Journal of the National Academy of Sciences 70: 3321–323.

Ostersen S, Foldager J and Hermansen J E. 1997. Effects of stage of lactation, milk protein genotype and body condition at calving on protein composition and renneting properties of bovine milk. Journal of Dairy Research 64: 207–19

Pal S, Woodford K and Kukuljan S. 2015. Milk intolerance, beta-casein and lactose. Nutrients 7(9): 7285–297.

Ramesha K P, Kumar A A, Kumar B P and Kushtagi V. 2015. β-casein polymorphism in Indian Bos indicus cattle using PCR-RFLP. Indian Journal of Veterinary Sciences and Biotechnology 11(1): 45–49.

Roginsky H. 2003. Encyclopedia of Dairy Sciences. Academic Press, London.

Sambrook J and Russell D. 2001. Molecular Cloning. A Laboratory Manual 3rd edition. Cold Spring Harbor Press, Cold Spring Harbour, N Y Sodhi, M Mukesh, M Mishra B P, Kishore A, Prakash B, Kapila R and Mclachlan C N S. 2001. CNS â-casein A1, ischaemic heart disease mortality, and other illnesses. Medical Hypoth 56: 262–72.

Sambrook J, Fritsch E F and Maniatis T.1989. Molecular Cloning: A laboratory manual. Harbor Laboratory Press, Cold Spring Harbor, New York. pp 21–26.

Shende S S, Waskar V S and Chaudhari C F. Molecular characterization of A1/A2 β-casein alleles in HF crossbred cattle. Indian Journal of Dairy Science 70(4): 478–81.

Snedecor G W and Cochran W G. 1976. Statistical methods. 6th ed. The Iowa State University Press.

Srinivas B. 2018. ‘Genotyping and protein profiling of milk beta-casein variants (A1 and A2) in Indian breeds of milch cattle.’ MSc thesis, Sri Venkateswara Veterinary University, Tirupati. Sun Z, Zhang Z, Wang X, Cade R, Elmer Z and Fregly M. 2003. Relation of beta-casomorphin to apnea in sudden infant death syndrome. Peptides 24: 937–43.

Tailford K A, Berry C L and Thomas A C. 2003. Beta- casomorphins and related peptides: Importance in the assessment of milk intolerance in the pediatric population. The Journal of Pediatrics 142(2): 142–44.