Effect of in-ovo injection of lavender oil and dietary supplementation of lavender powder on growth, intestine histomorphology, and serum parameters in broilers

SOLEEN MUSTAFA  SALMAN; SHAWKAT A  M’SADEQ; ABDULLAH  CAN

doi:10.56093/ijans.v94i12.149204

Authors

SOLEEN MUSTAFA SALMAN University of Duhok, College of Agriculture Engineering Sciences, Department of Animal Production, Iraq
SHAWKAT A M’SADEQ Agriculture Office, Duhok, Iraq
ABDULLAH CAN Harran University, Animal Science Department, Turkey

https://doi.org/10.56093/ijans.v94i12.149204

Keywords:

Blood biochemistry, Histomorphology, Lavender, Meat chicken

Abstract

The research was conducted at Animal Production Department, College of Agricultural Engineering Sciences, University of Duhok, Iraq. A total of 400 Ross 308 eggs were incubated, and 348 chicks were hatched. From the hatched chicks, 240 were randomly divided into 5 treatment groups, each with 4 replicates and 12 birds per pen. The treatments included a control group (no additive or injection), eggs injected with 0.05 mL of Lavender essential oil (T₁), eggs injected with 0.1 mL of lavender essential oil (T₂), birds fed with 0.5% lavender powder (T₃), and birds fed with 1% lavender powder (T₄). Results indicated that during days 0-10, birds injected with lavender essential oil (T₁) exhibited significantly higher body weight gain as compared to T₃ and T₄. By day 24, the body weight of birds in T₂ was significantly increased compared to control and T₄. Treatments T₁, and T₃ showed significantly higher body weight gain compared to T₄. Additionally, T₁ and T₂ reported significantly higher body weight and increased feed intake with no significant differences in feed conversion ratio among treatments by day 35. Villi height, crypt depth, tip, basal, and villi/crypt ratio was increased significantly in birds of T₁ group compared to those fed a control diet. All treatments showed a higher villi height/crypt depth ratio over the control. The concentration of AST serum in control group was higher than overall treatments. It was concluded that the in-ovo injection and dietary supplementation of lavender essential oil or powder had a positive effect on broiler performance and gut health.

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References

Abd El-Hack M E, AboElMaati M F, Abusudah W F, Awlya O F, Almohmadi N H, Fouad W, Mohamed H S, Youssef I M, Al-Gabri N A and Othman S I. 2024. Consequences of dietary cinnamon and ginger oils supplementation on blood biochemical parameters, oxidative status, and tissue histomorphology of growing Japanese quails. Poultry Science 103(2): 103314. DOI: https://doi.org/10.1016/j.psj.2023.103314

Adaszyńska‐Skwirzyńska M and Szczerbińska D. 2018. The antimicrobial activity of lavender essential oil (Lavandula angustifolia) and its influence on the production performance of broiler chickens. Journal of Animal Physiology and Animal Nutrition 102(4): 1020–25. DOI: https://doi.org/10.1111/jpn.12907

Alagawany M, Elnesr S S, Farag M R, Abd El-Hack M E, Barkat R A, Gabr AA, Foda M A, Noreldin A E, Khafaga AF and El-Sabrout K. 2021. Potential role of important nutraceuticals in poultry performance and health-A comprehensive review. Research in Veterinary Science 137(10): 9–29. DOI: https://doi.org/10.1016/j.rvsc.2021.04.009

Amad AA, Wendler K and Zentek J. 2013. Effects of a phytogenic feed additive on growth performance, selected blood criteria and jejunal morphology in broiler chickens. Emirates Journal of Food and Agriculture 25(7): 549–54. DOI: https://doi.org/10.9755/ejfa.v25i7.12364

Aviagen. 2012. Ross 308 Broiler Performance Objectives. In- house publication, global. Aviagen Ltd.,Newbridge, UK.

Barbarestani S Y, Jazi V, Mohebodini H, Ashayerizadeh A, Shabani A and Toghyani M. 2020. Effects of dietary lavender essential oil on growth performance, intestinal function, and antioxidant status of broiler chickens. Livestock Science 233: 103958. DOI: https://doi.org/10.1016/j.livsci.2020.103958

Barry S, Nakamura T, Matsuoka Y, Straub C, Horch H W and Extavour C G. 2019. Injecting Gryllus bimaculatus eggs. Journal of Visualized Experiments: JoVE (150): 10.3791/59726.

Beski S, M’Sadeq S, Hussein S and Mahmod A. 2021. Performance and physiological responses of broiler chickens to dietary combination of yeast and medical plants. Iraqi Journal of Agricultural Sciences 52(5): 1117–25. DOI: https://doi.org/10.36103/ijas.v52i5.1450

Bölükbaşı Ş C, Erhan M K and Kaynar Ö. 2008. The effect of feeding thyme, sage and rosemary oil on laying hen performance, cholesterol and some proteins ratio of egg yolk and Escherichia coli count in feces. Arch fur Geflugelkd 72: 231–37.

Brenes A and Roura E. 2010. Essential oils in poultry nutrition: Main effects and modes of action. Animal Feed Science and Technology 158(1-2): 1–14. DOI: https://doi.org/10.1016/j.anifeedsci.2010.03.007

Guidi L and Landi M. 2014. Aromatic Plants: use and nutraceutical properties. Novel Plant Bioresources: Applications in Food, Medicine and Cosmetics: 303–45. DOI: https://doi.org/10.1002/9781118460566.ch23

Hamid S A. 2023. Nanoencapsulated plant based bioactive compounds as novel substitute to antibiotic growth promoters in broiler chicken. University of Agricultural Sciences and Technology of Kashmir.

Hammer K A, Carson C F and Riley T V. 1999. Antimicrobial activity of essential oils and other plant extracts. Journal of Applied Microbiology 86(6): 985–90. DOI: https://doi.org/10.1046/j.1365-2672.1999.00780.x

Hasan H and M’Sadeq S. 2020. Effect of peppermint supplementation as powder or extract on broiler performance, serum biochemical content and gut health under e coli challenge. The Iraqi Journal of Agricultural Science 51(1): 299–310. DOI: https://doi.org/10.36103/ijas.v51i1.928

Hossain M S, Small B C, Kumar V and Hardy R. 2023. Utilization of functional feed additives to produce cost‐effective, ecofriendly aquafeeds high in plant‐based ingredients. Reviews in Aquaculture. DOI: https://doi.org/10.1111/raq.12824

Hussein S M, M’Sadeq S A, Beski S S M, Mahmood A L and Frankel T L. 2021. Different combinations of peppermint, chamomile and a yeast prebiotic have different impacts on production and severity of intestinal and bursal abnormalities of broilers challenged with coccidiosis. Italian Journal of Animal Science 20(1): 1924–34. DOI: https://doi.org/10.1080/1828051X.2021.1983479

Khishtan A, M’Sadeq S, Beski S and Omar S A. 2024. Effect of adding chlorogenic acid on broiler performance, some blood biochemical parameters and gut health. Iraqi Journal of Agricultural Sciences 55(2): 652–58. DOI: https://doi.org/10.36103/trrq9j63

Kiyma Z, Küçükyilmaz K, Çetinkaya M, Ateş A, Atalay H, Akdağ A, Bozkurt M and Gürsel F. 2017. Effect of lavender (Lavandula stoechas) essential oil on growth performance, carcass characteristics, meat quality and antioxidant status of broilers. South African Journal of Animal Science 47(2): 178–86. DOI: https://doi.org/10.4314/sajas.v47i2.9

M’sadeq S. 2019. Effect of dietary supplementation of miaclost on performance and gut morphology in broiler chickens challenged with escherichia coli. Iraqi Journal of Agricultural Sciences 2(50): 506–14.

M’Sadeq S A. 2023. Ameliorative effect of yeast cell walls on broiler chickens’ performance and gut health under coccidiosis challenge. Czech Journal of Animal Science 68(8): 346–55. DOI: https://doi.org/10.17221/24/2023-CJAS

Mathlouthi N, Bouzaienne T, Oueslati I, Recoquillay F, Hamdi M, Urdaci M and Bergaoui R. 2012. Use of rosemary, oregano, and a commercial blend of essential oils in broiler chickens: in vitro antimicrobial activities and effects on growth performance. Journal of Animal Science 90(3): 813–23. DOI: https://doi.org/10.2527/jas.2010-3646

Mokhtari K, Dimitrov D M and Reichard C A. 2018. Revising the “Metacognitive Awareness of Reading Strategies Inventory”(MARSI) and testing for factorial invariance. Studies in Second Language Learning and Teaching 8(2): 219–46. DOI: https://doi.org/10.14746/ssllt.2018.8.2.3

Murugesan G R, Syed B, Haldar S and Pender C. 2015. Phytogenic feed additives as an alternative to antibiotic growth promoters in broiler chickens. Frontiers in veterinary science 2(21): 1–21. DOI: https://doi.org/10.3389/fvets.2015.00021

Nasiri-Moghaddam H, Hassanabadi A and Bidar N. 2012. Effects of increasing levels of lavender essential oil (Lavandula angustifolia) on performance and hematological traits of broilers. Iranian Journal of Animal Science Research 4: 115–21.

Oladokun S, MacIsaac J, Rathgeber B and Adewole D. 2021. Essential oil delivery route: Effect on broiler chicken’s growth performance, blood biochemistry, intestinal morphology, immune, and antioxidant status. Animals 11(12): 3386. DOI: https://doi.org/10.3390/ani11123386

Salajegheh A, Salarmoini M, Afsharmanesh M and Salajegheh M. 2018. Growth performance, intestinal microflora, and meat quality of broiler chickens fed lavender (Lavandula angustifolia) powder. Journal of Livestock Science and Technologies 6(1): 31–38.

Salarmoini M, Salajegheh A, Salajegheh M and Afsharmanesh M. 2019. The effect of lavender (Lavandula angustifolia) extract in comparison to antibiotic on growth performance, intestinal morphology, ileal microflora, antioxidant status and meat quality of broilers. Iranian Journal of Applied Animal Science 9(4): 717–25.

Sarker M S, Mannan M S, Ali M Y, Bayzid M, Ahad A and Bupasha Z B. 2019. Antibiotic resistance of Escherichia coli isolated from broilers sold at live bird markets in Chattogram, Bangladesh. Journal of Advanced Veterinary and Animal Research 6(3): 272. DOI: https://doi.org/10.5455/javar.2019.f344

Torki M, Mohebbifar A and Mohammadi H. 2021. Effects of supplementing hen diet with Lavandula angustifolia and/ or Mentha spicata essential oils on production performance, egg quality and blood variables of laying hens. Veterinary Medicine and Science 7(1): 184–93. DOI: https://doi.org/10.1002/vms3.343