Molecular detection of major coagulase positive Staphylococcus isolates from the canine pyoderma cases

KHAMBAM  SHOBHA; GHODASARA  S N; BARAD  D B; JAVIA  B B; FEFAR  D T; POSHIYA  P J

doi:10.56093/ijans.v94i6.149258

Authors

KHAMBAM SHOBHA College of Veterinary Science and Animal Husbandry, Kamdhenu University, Junagadh, Gujarat 362 001 India
GHODASARA S N College of Veterinary Science and Animal Husbandry, Kamdhenu University, Junagadh, Gujarat 362 001 India
BARAD D B College of Veterinary Science and Animal Husbandry, Kamdhenu University, Junagadh, Gujarat 362 001 India
JAVIA B B College of Veterinary Science and Animal Husbandry, Kamdhenu University, Junagadh, Gujarat 362 001 India
FEFAR D T College of Veterinary Science and Animal Husbandry, Kamdhenu University, Junagadh, Gujarat 362 001 India
POSHIYA P J College of Veterinary Science and Animal Husbandry, Kamdhenu University, Junagadh, Gujarat 362 001 India

https://doi.org/10.56093/ijans.v94i6.149258

Keywords:

Canine pyoderma, Staphylococcus aureus, Staphylococcus pseudintermedius, Staphylococcus schleiferi subsp. coagulans

Abstract

Pyoderma is a one of the most common dermatological condition frequently observed in small animal practice. The genus Staphylococcus is responsible for approximately 90% of pyoderma cases in dogs, caused by bacterial infection. The purpose of the present study was to investigate the prevalence of major coagulase positive Staphylococcus bacteria causing different types of canine pyoderma in dog breeds. The samples were collected from the clinical cases of canine pyoderma with varying grade from the different breeds of dogs for the molecular based detection of major coagulase-positive Staphylococcus species. Out of total bacterial isolates, majority of them were molecularly identified as Staphylococcus spp. by 16S rRNA gene. Of these Staphylococcus spp., most common isolated organism was S. pseudintermedius (80.82%) followed by S. schleiferi subsp. coagulans (8.22%) and aureus (2.74%), using Pse-nuc, Sch-nuc, and Au-nuc genes, respectively.

Downloads

Download data is not yet available.

References

Abusleme F, Galarce N, Quezada-Aguiluz M, Iragüen D and Gonzalez-Rocha G. 2022. Characterization and antimicrobial susceptibility of coagulase-positive Staphylococcus isolated in a veterinary teaching hospital in Chile. Revista Argentina de Microbiologia 54: 192–202.

Chaudhary A K, Kumar A and Shrivastva M. 2019. Study on prevalence and resistance patterns of bacterial pathogens isolated from canine pyoderma. International Journal of Current Microbiology and Applied Science 8(1): 2305–11.

Chaudhary S S, Chauhan H. C, Sharma K K, Patel S S, Mohapatra S K, Patel A C, Shrimali M D and Chandel B S. 2021. Bacteriological and molecular identification and characterization of Staphylococcus aureus from different affections of canines. Indian Journal of Animal Research 55(4): 474–78.

DeBoer D J and Marsella R. 2001. The ACVD task force on canine atopic dermatitis (XII): The relationship of cutaneous infections to the pathogenesis and clinical course of canine atopic dermatitis. Veterinary Immunology and Immunopathology 81(3-4): 239–49.

Dziva F, Wint C, Auguste T, Heeraman C, Dacon C, Yu P and Koma L M. 2015. First identification of methicillin-resistant Staphylococcus pseudintermedius strains among coagulase- positive staphylococci isolated from dogs with otitis externa in Trinidad, West Indies. Infection Ecology and Epidemiology 5: 29170.

Fadhil H and Mohammed B J. 2022. Molecular and classical identification of Staphylococcus aureus, isolated from Iraqi patients with recurrent tonsillitis. Medico-Legal Update 22(1): 23.

Gonzalez-Dominguez M S, Carvajal H D, Calle-Echeverri D A and Chinchilla-Cardenas D. 2020. Molecular detection and characterization of the mecA and nuc genes from Staphylococcus species (S. aureus, S. pseudintermedius, and S. schleiferi) isolated from dogs suffering superficial pyoderma and their antimicrobial resistance profiles. Frontiers in Veterinary Science 7: 376.

Hill P B, Lo A, Eden C A, Huntley S, Morey V, Ramsey S, Richardson C, Smith D J, Sutton C, Taylor M D, Thorpe E, Tidmarsh R and Williams V. 2006. Survey of the prevalence, diagnosis and treatment of dermatological conditions in small animals in general practice. The Veterinary Record 158(16): 533–39.

Hritcu O M, Schmidt V M, Salem S E, Maciuca I E, Moraru R F, Lipovan I, Mareş M, Solcan G and Timofte D. 2020. Geographical variations in virulence factors and antimicrobial resistance amongst staphylococci isolated from dogs from the United Kingdom and Romania. Frontiers in Veterinary Science 7: 414.

Igbinosa E O, Beshiru A, Akporehe L U and Ogofure A G. 2016. Detection of methicillin-resistant staphylococci isolated from food producing animals: A public health implication. Veterinary Science 3(3): 14.

Jane E S, Terry M N and Stephen D W. 2014. Canine and feline infectious diseases: Pyoderma, otitis externa, and otitis media,

pp. 800–13. Canine and Feline Infectious Diseases. (Ed) Jane E S. W. B. Saunders.

Kadhim H B and Abdullah A H. 2022. Molecular detection of Staphylococcus pseudintermedius isolated from otitis in dogs. Annals of Forest Research 65(1): 10884–0900.

Kawakami T, Shibata S, Murayama N, Nagata M, Nishifuji K, Iwasaki T and Fukata T. 2010. Antimicrobial susceptibility and methicillin resistance in Staphylococcus pseudintermedius and Staphylococcus schleiferi subsp. coagulans isolated from dogs with pyoderma in Japan. Journal of Veterinary Medical Science 72(12): 1615–19.

Khoshnegah J, Movassaghi A R and Rad M. 2013. Survey of dermatological conditions in a population of domestic dogs in Mashhad, northeast of Iran (2007-2011). Veterinary Research Forum 4(2): 99–103.

Lautz S, Kanbar T, Alber J, Lammler C, Weiss R, Prenger- Berninghoff E and Zschock M. 2006. Dissemination of the gene encoding exfoliative toxin of Staphylococcus intermedius among strains isolated from dogs during routine microbiological diagnostics. Journal of Veterinary Medicine. B, Infectious Disease and Veterinary Public Health 53(9): 434–38.

Loeffler A and Lloyd D H. 2018. What has changed in canine pyoderma? A narrative review. The Veterinary Journal 235: 73–82.

Lynch S A and Helbig K J. 2021. The complex diseases of Staphylococcus pseudintermedius in canines: Where to next? The Veterinary Journal 8(1): 11.

Martineau F, Picard F J, Ke D, Paradis S, Roy P H, Ouellette M and Bergeron M G. 2001. Development of a PCR assay for identification of staphylococci at genus and species levels. Journal of Clinical Microbiology 39(7): 2541–47.

Mathapati B S, Nimavat V, Javia B, Barad D, Jivani H and Maharana B. 2016. Isolation and characterisation of multi- drug resistant coagulase negative S. schleiferi subsp. schleferi from a case of recurrent pyoderma in canine. Journal of Immunology and Immunopathology 18: 39–46.

Nakaminami H, Okamura Y, Tanaka S, Wajima T, Murayama N and Noguchi N. 2021. Prevalence of antimicrobial-resistant staphylococci in nares and affected sites of pet dogs with superficial pyoderma. The Journal of Veterinary Medical Science 83(2): 214–19.

Nocera F P, Ambrosio M, Fiorito F, Cortese L and De Martino L. 2021. On Gram-Positive and Gram-Negative bacteria- associated canine and feline skin infections: A 4-year retrospective study of the university veterinary microbiology diagnostic laboratory of naples, Italy. Animals 11(6): 1603.

Quinn P J, Markey B K, Leonard F C, Hartigan P, Fanning S and Fitzpatrick E. 2011. Veterinary Microbiology and Microbial Disease. John Wiley and Sons.

Rana E A, Islam M Z, Das T, Dutta A, Ahad A, Biswas P K and Barua H. 2022. Prevalence of coagulase-positive methicillin- resistant Staphylococcus aureus and Staphylococcus pseudintermedius in dogs in Bangladesh. Veterinary Medicine and Science 8(2): 498–508.

Sambrook J and Russell D W. 2001. Molecular Cloning: A Laboratory Manual. 2 (3rd Ed.). Cold Spring Harbor Laboratory Press, New York.

Sasaki T, Tsubakishita S, Tanaka Y, Sakusabe A, Ohtsuka M, Hirotaki S, Kawakami T, Fukata T and Hiramatsu K. 2010. Multiplex-PCR method for species identification of coagulase- positive staphylococci. Journal of Clinical Microbiology 48(3): 765–69.

Schmidt V M, Williams N J, Pinchbeck G, Corless C E, Shaw S, McEwan N, Dawson S and Nuttall T. 2014. Antimicrobial resistance and characterisation of staphylococci isolated from healthy Labrador retrievers in the United Kingdom. BMC Veterinary Research 10: 17.

Senapati S K, Patra R C and Panda H K. 2014. Prevalence and antibiogram of bacterial pathogens isolated from canine pyoderma. The Indian Journal of Veterinary Science and Biotechnology 9(3): 41–45.

Shah B, Mathakiya R, Rao N and Nauriyal D S. 2017. Organisms recovered from cases of canine pyoderma and their antibiogram pattern. Journal of Animal Research 7(6): 1067–73.

Soedarmanto I, Kanbar T, Ulbegi-Mohyla H, Hijazin M, Alber J, Lammler C, Akineden O, Weiss R, Moritz A and Zschock M. 2011. Genetic relatedness of methicillin-resistant Staphylococcus pseudintermedius (MRSP) isolated from a dog and the dog owner. Research in Veterinary Science 91(3): e25–e27.

Somayaji R, Priyantha M A, Rubin J E and Church D. 2016. Human infections due to Staphylococcus pseudintermedius, an emerging zoonosis of canine origin: Report of 24 cases. Diagnostic Microbiology and Infectious Disease 85(4): 471–76.

Tamakan H and Gocmen H. 2022. Genetic characterization of methicillin resistant Staphylococcus pseudintermedius in dogs and cats in Cyprus: Comparison of MRSP and MRSA results. Pakistan Journal of Zoology 54(4): 1511.

Viegas F M, Santana J A, Silva B A, Xavier R G C, Bonisson C T, Câmara J L S, Renno M C, Cunha J L R, Figueiredo H C P, Lobato F C F and Silva R O S. 2022. Occurrence and characterization of methicillin-resistant Staphylococcus spp. in diseased dogs in Brazil. PloS One 17(6): 1–17.