Buffalo- calf gut origin Pediococcus shows superior probiotic potential than Lactobacillus under in vitro system

ANJU  KALA; KAVIPRIYA  JAYSWAL; L C  CHAUDHARY; PAYAL  AGARWAL; NEETA  AGARWAL; ZB  DUBAL

doi:10.56093/ijans.v95i10.150307

Authors

ANJU KALA ICAR-Indian Veterinary Research Institute, Izatnagar 243 122 (UP), India
KAVIPRIYA JAYSWAL ICAR-Indian Veterinary Research Institute, Izatnagar 243 122 (UP), India
L C CHAUDHARY ICAR-Indian Veterinary Research Institute, Izatnagar 243 122 (UP), India
PAYAL AGARWAL ICAR-Indian Veterinary Research Institute, Izatnagar 243 122 (UP), India
NEETA AGARWAL ICAR-Indian Veterinary Research Institute, Izatnagar 243 122 (UP), India
ZB DUBAL ICAR-Indian Veterinary Research Institute, Izatnagar 243 122 (UP), India

https://doi.org/10.56093/ijans.v95i10.150307

Keywords:

Lactobacillus, Murrah Buffalo calf, Pediococcus, Probiotic

Abstract

Autochthonous probiotics are preferred over allochthonous probiotics. Therefore, this research was aimed to isolate and characterize lactic acid bacteria (LAB) from buffalo-calves faeces and evaluate their probiotic potential to develop an autochthonous probiotic for buffalo. Thirty two LABs were isolated from Murrah buffalo-calves faeces out of which eight isolates exhibiting maximum hydrophobicity and minimum auto-aggregation, were subjected to further characterization. Out of 8 two isolates, RM119 and RM122, exhibited highest cell surface hydrophobicity, minimum aggregation time, highest lactic acid production, resistance to various temperatures low pH and bile salts. However, antagonism against Escherichia coli ATCC 25922, Klebsiella pneumoniae ATCC 70063, and Staphylococcus aureus was higher with RM119 as compared to RM122. Phylogenetic analysis revealed that the isolates RM103, RM104, RM106, RM107 and RM113 (NCBI Genbank accession no MN880194, MT180734, MN880195, MN880196 and MT907286, respectively), belonged to Lactiplantibacillus fermentum, isolate RM 102 belonged to Lactobacillus plantarum and RM119 and RM122 belonged to Pediococcus pentosaceus. The results revealed that the two strains of P. pentosaceus (RM112 and RM119) were superior over Lactobacillus isolates in terms of probiotic potential.

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