Occurrence and characterization of multidrug-resistant extended spectrum β-lactamase producing Escherichia coli from bovine mastitis of Haryana

JINU  MANOJ; SARIN  KAMBOJ; RAJESH  CHHABRA; MAHAVIR  SINGH

doi:10.56093/ijans.v94i6.150486

Authors

JINU MANOJ Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana 125 001 India
SARIN KAMBOJ Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana 125 001 India
RAJESH CHHABRA Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana 125 001 India
MAHAVIR SINGH Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana 125 001 India

https://doi.org/10.56093/ijans.v94i6.150486

Keywords:

Antibiotic resistance, DDST, ESBL, Gram negative, MAR index

Abstract

A study was carried out to analyze 500 bovine milk samples collected from Haryana, India and 37% samples were found to be associated with E. coli as a causative agent for mastitis. Out of it, 53 (10.6%) isolates were found to be positive for ESBL production by Double-Disk Synergy test and Epsilon test. However, the ESBL-encoding gene blaCTX-M was detected in the 51 (10.2%) isolates. The antibiotic susceptibility test revealed 13.72% of the isolates were multidrug resistant, with MAR index varying from 0.06 to 0.46. The maximum sensitivity was observed towards gentamicin (90.19%) followed by chloramphenicol (78.4%). Fluoroquinolones antibiotics were found to be resistant between 84.31% to 76.47%. The resistance profile underscores the importance of antibiotic stewardship and the need for ongoing surveillance using one health approach to guide therapy and manage the spread of resistant bacteria.

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