Characterization of isolates of Staphylococcus aureus for biofilm formation in goats of Aizawl, Mizoram

C M S  CHOWPU; A  KUMAR; P  BEHERA; L  RALTE; T K  DUTTA; J  LALMUANPUIA

doi:10.56093/ijans.v95i3.151048

Authors

C M S CHOWPU College of Veterinary Sciences and Animal Husbandry, Central Agricultural University (Imphal), Selesih, Aizawl, Mizoram- 796015
A KUMAR College of Veterinary Sciences and Animal Husbandry, Central Agricultural University (Imphal), Selesih, Aizawl, Mizoram- 796015
P BEHERA College of Veterinary Sciences and Animal Husbandry, Central Agricultural University (Imphal), Selesih, Aizawl, Mizoram- 796015
L RALTE College of Veterinary Sciences and Animal Husbandry, Central Agricultural University (Imphal), Selesih, Aizawl, Mizoram- 796015
T K DUTTA College of Veterinary Sciences and Animal Husbandry, Central Agricultural University (Imphal), Selesih, Aizawl, Mizoram- 796015
J LALMUANPUIA College of Veterinary Sciences and Animal Husbandry, Central Agricultural University (Imphal), Selesih, Aizawl, Mizoram- 796015

https://doi.org/10.56093/ijans.v95i3.151048

Keywords:

icaA, icaD, Goat, Mizoram, S. aureus

Abstract

Staphylococcus aureus are the inhabitants of intricate biofilms holding multiple layers of bacteria embedded inside its extracellular matrix. The development of biofilms is a substantial factor that contributes to the failure of treatments. It is recommended to employ a combination of phenotypic and genotypic approaches in the study of biofilm formation in S. aureus. Among the 43 S. aureus isolates obtained from goats, both culturally and genotypically characterized, it was shown that 14 isolates (32.55%) exhibited the presence of the biofilm-producing gene icaD, while none of the isolates tested positive for the presence of icaA. However, out of the total 32 isolates, which accounts for approximately 74.41% of the sample, biofilm formation was seen using the crystal violet tube adherence method. The findings of this study demonstrate a notable prevalence of the ica genes among Staphylococcus aureus isolates derived from goats. Furthermore, it is seen that the presence of these genes does not consistently correlate with the in vitro development of biofilm. The capacity to produce biofilms can result in treatment inefficacy, posing a significant risk to public health and animal welfare, while also causing financial losses for farmers.

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References

Andrade N C, Laranjo M, Costa M M and Queiroga M C. 2021. Virulence Factors in Staphylococcus Associated with Small Ruminant Mastitis: Biofilm Production and Antimicrobial Resistance Genes. Antibiotics (Basel)10(6): 633.

Brakstad OG, Aasbakk K and Maelamd JA. 1992. Detection of Staphylococcus aureus by polymerase chain reaction amplification of nuc gene. Journal of Clinical Microbiology 30(7): 1654-60.

Cramton S E, Gerke C, Schnell N F, Nichols W W and Götz F. 1999. The intercellular adhesion (ica) locus is present in Staphylococcus aureus and is required for biofilm formation. Infection and immunity 67(10): 5427-33.

Dumaru R, Baral R and Shrestha L B. 2019. Study of biofilm formation and antibiotic resistance pattern of gram-negative Bacilli among the clinical isolates at BPKIHS, Dharan. BMC Research Notes 12(1): 38. doi: 10.1186/s13104-019-4084-8.

Flemming H C, Wingender J, Szewzyk U, Steinberg P, Rice S A, and Kjelleberg S. 2016. Biofilms: an emergent form of bacterial life. Nature Reviews Microbiology 14(9): 563-75.

Liberto MC, Matera G, Quirino A, Lamberti AG, Capicotto R, Puccio R, Barreca GS, Focà E, Cascio A and Focà A. 2009. Phenotypic and genotypic evaluation of slime production by conventional and molecular microbiological techniques. Microbiolgical Research 64(5): 522-8. doi: 10.1016/j.micres.2007.04. 004.

Lira M C, Givisiez P E, De Sousa F G, Magnani M, De Souza E L, Spricigo D A, Gebreyes W A and De Oliveira C J. 2016. Biofilm-forming and antimicrobial resistance traits of staphylococci isolated from goat dairy plants. The Journal of infection in Developing Countries 10(9): 932-8.

Monecke S, Gavier-Widen D, Hotzel H, Peters M, Guenther S, Lazaris A, Loncaric I, Muller E, Reissig A, Ruppelt-Lorz A, Shore AC, Walter B, Coleman D C and Ehricht R. 2016. Diversity of Staphylococcus aureus isolates in European wildlife. PLoS One 11(12): e168433.

Salimena A P, Lange C C, Camussone C, Signorini M, Calvinho L F, Brito M A, Borges C A, Guimarães A S, Ribeiro J B, Mendonça L C and Piccoli R H. 2016. Genotypic and phenotypic detection of capsular polysaccharide and biofilm formation in Staphylococcus aureus isolated from bovine milk collected from Brazilian dairy farms. Veterinary Research Communication 40(3-4):9 7-106.

Satorres S E and Alcaráz L E. 2007. Prevalence of icaA and icaD genes in Staphylococcus aureus and Staphylococcus epidermidis strains isolated from patients and hospital staff. Central European Journal of Public Health 15(2): 87-90.

Simojoki H, Hyvönen P, Plumed Ferrer C, Taponen S and Pyörälä S. 2012. Is the biofilm formation and slime producing ability of coagulase-negative staphylococci associated with the persistence and severity of intramammary infection? Veterinary Microbiology 158(3-4): 344-52.

Smith T C. 2015. Livestock-Associated Staphylococcus aureus: The United States Experience. PLOS Pathology 11(2): e1004564.doi: 10.1371/journal.ppat.1004564

Tormo M Á, Knecht E, Götz F, Lasa I and Penadés J R. 2005. Bap-dependent biofilm formation by pathogenic species of Staphylococcus: evidence of horizontal gene transfer? Microbiology (Reading) 151(Pt 7): 2465-75. doi: 10.1099/ mic.0.27865-0. PMID: 16000737.

Vasudevan P, Nair M K, Annamalai T and Venkitanarayanan K S. 2003. Phenotypic and genotypic characterization of bovine mastitis isolates of Staphylococcus aureus for biofilm formation. Veterinary Microbiology 92(1-2): 179-85.

Xu J, Tan X, Zhang X, Xia X and Sun H. 2015. The diversities of staphylococcal species, virulence and antibiotic resistance genes in the subclinical mastitis milk from a single Chinese cow herd. Microbiology Pathology 88: 29-38.