Molecular detection of Brucella melitensis in sheep from district Budgam, Jammu and Kashmir

KHALID  BASHIR; TUFAIL  HUSSAIN; NOUSHEEN  QADRI; JAVAID AHMAD  BABA; IMRAN HAMID  SHAH; MUBASHIR ALI  RATHER

doi:10.56093/ijans.v94i8.151333

Authors

KHALID BASHIR Department of Sheep Husbandry, Srinagar, Jammu and Kashmir
TUFAIL HUSSAIN Department of Sheep Husbandry, Srinagar, Jammu and Kashmir
NOUSHEEN QADRI Disease Investigation Laboratory, Nowshera, Srinagar, Jammu and Kashmir
JAVAID AHMAD BABA Disease Investigation Laboratory, Nowshera, Srinagar, Jammu and Kashmir
IMRAN HAMID SHAH Disease Investigation Laboratory, Nowshera, Srinagar, Jammu and Kashmir
MUBASHIR ALI RATHER Disease Investigation Laboratory, Nowshera, Srinagar, Jammu and Kashmir

https://doi.org/10.56093/ijans.v94i8.151333

Keywords:

Abortion, Brucella melitensis, PCR, Sheep, Zoonotic

Abstract

Farmers reported abortions and stillbirths in 600 sheep including 471 pregnant ewes. A complete investigation including physical examination of livestock, postmortem examination of stillbirths, serological examination of ewes and molecular study of abomasal contents, peritoneal fluid, liver and kidney tissue collected from still born lambs was done to ascertain the cause of mortality at Disease Investigation Laboratory, Nowshera, Srinagar. Also, on spot heat fixed tissue smears were prepared for Gram’s staining and accordingly observed for presence of organism. Extraction of bacterial genomic DNA from samples from still born lambs was performed and obtained DNA extracts were checked by agarose gel electrophoresis and stored at -20°C till further use. The PCR conducted on the abomasal, peritoneal contents and liver and kidney tissues revealed 731 bp band after the gel electrophoresis, confirming Brucella melitensis as cause of stillbirths in the flock. The investigation revealed that Brucella melitensis was cause of stillbirths and abortions in sheep flocks.

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