Characterizing morphological heterogeneity in porcine hepatocytes

KIRPAL  SINGH; VARINDER  UPPAL; NEELAM  BANSAL; ANURADHA  GUPTA; DEVENDRA  PATHAK

doi:10.56093/ijans.v95i2.151817

Authors

KIRPAL SINGH Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, 141004- Punjab
VARINDER UPPAL Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, 141004- Punjab
NEELAM BANSAL Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, 141004- Punjab
ANURADHA GUPTA Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, 141004- Punjab
DEVENDRA PATHAK Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, 141004- Punjab

https://doi.org/10.56093/ijans.v95i2.151817

Keywords:

Hepatocytes, Heterogeneity, Morphology, Pig

Abstract

The present study was conducted on liver of eight pigs collected from local slaughter house, for gross anatomical, histological, histochemical, immunohistochemical and histoenzymic studies. The paraffin sections of 3 µm were cut and stained with different histomorphochemical stains. The cryostat sections of fresh frozen tissues of 10 µm thickness were cut at -20˚C and incubated in different substrates to localize phosphatases and Non-specific esterases. The left lateral lobe was significantly larger and quadrate lobe was shortest. The size of hepatocytes in periportal area was significantly more than size of hepatocytes in centrilobular area. The number of mononuclear hepatocytes was significantly more in periportal area than centrilobular area whereas number of binucleated hepatocytes didn’t vary significantly between periportal and centrilobular areas. The average no. of Kupffer cells in periportal region was significantly more than centrilobular area. The reaction for neutral mucopolysaccharides and glycogen was more in centrilobular hepatocytes, proteins were distributed homogeneously and reaction for lipids was more in centrilobular hepatocytes. Periportal hepatocytes exhibited a strong phosphatases activity whereas non-specific esterases activity was more in centrilobular hepatocytes. This work will contribute to the corpus of knowledge regarding the heterogeneity of hepatocytes, which enables the liver to carry out a variety of functions and enhances its complexity and adaptability in preserving the body’s homeostasis.

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