Preparation, characterization and utilization of a novel feed supplement in poultry

SRIDHAR  S; MUTHUSAMY  N; KATHIRVELAN  C; KUMARESAN  G; VASANTHAKUMAR  P; SENTHILKUMAR  R P

doi:10.56093/ijans.v94i9.152121

Authors

SRIDHAR S Veterinary College and Research Institute, Namakkal, Tamil Nadu
MUTHUSAMY N Krishi Vigyan Kendra, Veterinary College and Research Institute Campus, Namakkal, Tamil Nadu
KATHIRVELAN C Veterinary College and Research Institute, Udumalpet, Tamil Nadu
KUMARESAN G Veterinary College and Research Institute, Namakkal, Tamil Nadu
VASANTHAKUMAR P Veterinary College and Research Institute, Namakkal, Tamil Nadu
SENTHILKUMAR R P Veterinary College and Research Institute, Namakkal, Tamil Nadu

https://doi.org/10.56093/ijans.v94i9.152121

Keywords:

FTIR, SEM-EDAX, Zinc alginate complex, Zinc retention, XRD

Abstract

This study aimed to prepare and characterize the zinc alginate complexes using SEM-EDAX, XRD, FTIR and evaluate their zinc retention percentage in cockerels. Three complexes were prepared using zinc sulfate (Zn-Alg- CM₁), zinc carbonate (Zn-Alg-CM₂), and zinc nitrate (Zn-Alg-CM₃). Zn-Alg-CM₃ was amorphous with smooth surfaces and showed a shifted absorption spectrum, indicating strong zinc binding compared to Zn-Alg-CM₁ and Zn- Alg-CM₂. Although Zn-Alg-CM₂ had higher zinc composition, Zn-Alg-CM₃ at 45 ppm significantly improved the digestibility of crude protein, crude fibre and zinc retention compared to Zn-Alg-CM₁, Zn-Alg-CM₂, and the control with zinc oxide in cockerels. Zn-Alg-CM₃ exhibited antibacterial activity against E. coli, Salmonella and S. aureus. Therefore, Zn-Alg-CM₃ showed promising potential as a novel feed supplement in cockerels and further research is essential to fully understand its impact on growth performance, antioxidant activity, and intestinal health in poultry.

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References

AAFCO. 2000. Feed terms and ingredient definitions. Association of American Feed Control Officials.

Abou O H, Amadine O, Essamlali Y, Dânoun K and Zahouily M. 2018. Supercritical CO2 drying of alginate/zinc hydrogels: A green and facile route to prepare ZnO foam structures and ZnO nanoparticles. RSC Advances 8(37): 20737–747.

Alkhayer G, Khudr H and Koudsi Y. 2020. Enantioselective release behaviour of ketoprofen enantiomers from alginate- metal complexes, monitored by chiral HPLC. Analytical and Bioanalytical Chemistry Research 7(1): 61–76.

AOAC. 2000. Official Methods of Analysis (17th Ed). Association of Official Analytical Chemists. Washington D.C., USA.

Basha S K, Lakshmi K V and Kumari V S. 2016. Ammonia sensor and antibacterial activities of green zinc oxide nanoparticles. Sensing and Bio-Sensing Research 10: 34–40.

BIS. 2007. Bureau of Indian Standards (5th Ed). Indian Standards for Poultry Feeds - Specifications, New Delhi, India.

Choudhury N, Meghwal M and Das K. 2021. Microencapsulation: An overview on concepts, methods, properties and applications in foods. Food Frontiers 2(4): 426–42.

Clinical and Laboratory Standards Institute. 2012. Performance Standards for Antimicrobial Disk Susceptibility Tests (11th Ed), Approved Standard-M02-A11, vol. 32, no. 1 Replaces M02-A10, vol. 29, no. 1, CLSI, Wayne, Pa, USA.

Fang M, Chen J H, Xu X L, Yang P H and Hildebrand H F. 2006. Antibacterial activities of inorganic agents on six bacteria associated with oral infections by two susceptibility tests. International Journal of Antimicrobial Agents 27(6): 513–17.

Hamouda R A, Alharbi A A, Al-Tuwaijri M M and Makharita R R. 2023. The antibacterial activities and characterizations of biosynthesized zinc oxide nanoparticles, and their coated with alginate derived from Fucus vesiculosus. Polymers 15(10): 2335.

Hassan R M. 1993. Alginate polyelectrolyte ionotropic gels. XIII geometrical aspects for chelation in metal alginate complexes related to their physico‐chemical properties. Polymer International 31(1): 81–86.

Jarosz Ł S, Michalak K, Marek A, Hejdysz M, Ciszewski A, Kaczmarek S, Kwiecień M and Grądzki Z. 2022. The effect of feed supplementation with zinc glycine chelate and zinc sulphate on hepatic proteome profiles in chickens. Livestock Science 262: 104983.

Makarova A O, Derkach S R, Khair T, Kazantseva M A, Zuev Y F and Zueva O S. 2023. Ion-induced polysaccharide gelation: Peculiarities of alginate egg-box association with different divalent cations. Polymers 15(5): 1243.

Mozhiarasi V, Karunakaran R, Raja P and Radhakrishnan L. 2024. Effects of zinc oxide nanoparticles supplementation on growth performance, meat quality and serum biochemical parameters in broiler chicks. Biological Trace Element Research 202(4): 1683–98.

Nandiyanto A B D, Oktiani R and Ragadhita R. 2019. How to read and interpret FTIR spectroscope of organic material. Indonesian Journal of Science and Technology 4(1): 97–118.

Papageorgiou S K, Kouvelos E P, Favvas E P, Sapalidis A A, Romanos G E and Katsaros F K. 2010. Metal–carboxylate interactions in metal–alginate complexes studied with FTIR spectroscopy. Carbohydrate Research 345(4): 469–73.

Pereira R, Tojeira A, Vaz D C, Mendes A and Bártolo P. 2011. Preparation and characterization of films based on alginate and aloe vera. International Journal of Polymer Analysis and Characterization 16(7): 449–64.

Ramirez S, Lu W B, Davin R, van der Broek H and Liu Y G. 2022. Effect of encapsulated trace minerals premix in comparison with inorganic and organic microminerals on growth performance and mineral excretion of broiler. Journal of Food Science and Nutrition Research 5(1): 341–50.

Rao S B N, Elangovan A V, Madiajagan B, Rajendran D, Franklin M E E, Gopi M, Pal D, Parthipan S, Nalina M, Dey D K and Manjunatha Reddy G B. 2023. Production and evaluation of encapsulated zinc oxide on performance, ileal digestibility and zinc transporter gene expression in broiler chicken. Biological Trace Element Research 201(12): 5774–85.

Said A A and Hassan R M. 1993. Thermal decomposition of some divalent metal alginate gel compounds. Polymer Degradation and Stability 39(3): 393–97.

Siddiqui V U, Ansari A, Ansari M T, Akram M K and Siddiqi W A. 2022. Fabrication of a zinc oxide/alginate (ZnO/Alg) bionanocomposite for enhanced dye degradation and its optimization study. RSC Advances 12(12): 7210–28.

Snedecor G W and Cochran W G. 1989. Statistical Methods, (8th Ed), Iowa State University Press, Ames, USA.

Straccia M C, d’Ayala G G, Romano I and Laurienzo P. 2015. Novel zinc alginate hydrogels prepared by the internal setting method with intrinsic antibacterial activity. Carbohydrate Polymers 125: 103–12.

Suttle N. 2022. Mineral Nutrition of Livestock, (5th Ed). Pp. 608. GB: Cabi.

Thomas D, Latha M S and Thomas K K. 2018. Zinc-alginate beads for the controlled release of Rifampicin. Oriental Journal of Chemistry 34(1).

Urbanova M, Pavelkova M, Czernek J, Kubova K, Vyslouzil J, Pechova A, Molinkova D, Vyslouzil J, Vetchy D and Brus J. 2019. Interaction pathways and structure–chemical transformations of alginate gels in physiological environments. Biomacromolecules 20(11): 4158–70.

Yu D G, Li J J, Williams G R and Zhao M. 2018. Electrospun amorphous solid dispersions of poorly water-soluble drugs: A review. Journal of Control Release 292: 91–110.

Yusof H M, Mohamad R, Zaidan U H, Arshad M A and Samsudin A A. 2023. Effects of dietary zinc oxide nanoparticles supplementation on broiler growth performance, zinc retention, liver health status, and gastrointestinal microbial load. Journal of Trace Elements and Minerals 4: 100072.