In-vitro assessment of carbaryl-induced immunotoxicity in chicken lymphocytes

Abstract

In present study, an insecticide, carbaryl was evaluated for in-vitro assessment of immunotoxicity in chicken lymphocytes. The splenic lymphocytes were collected and treated with NOEL/10², NOEL/10³, NOEL/10⁴, NOEL/10⁵, NOEL/10⁶ and NOEL/10⁷ dose of carbaryl for 30, 60 and 90 min and a group of cells were kept control. The Concanvalin A (Con-A) and lipopolysaccharide (LPS) stimulated proliferation of T and B lymphocytes were assessed using MTT dye method. The proliferation of T- and B-lymphocytes responses to Con-A and LPS were markedly reduced in treated group in comparison to control indicating the down regulation of not only the number of the variable lymphocytes but also their blastogenic activity required to mount immune response in chicken. It can be concluded from the present study that the carbaryl, even in very low doses can reduce the proliferation of chicken lymphocytes leading to state of immunosuppression.