Evaluation of tropical feedstuffs used in ruminant animals for carbohydrate and protein fractions by CNCP system
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Keywords:
Agro industrial by products, cereal grains, CNCPS, in vitro digestibility, methane production.Abstract
This research was carried out to assess the nutritional content of frequently used feedstuffs/agro industrial by products as per CNCPS along with in vitro digestibility, gas and methane production. Study was conducted on twenty one frequently used animal feed ingredients relating 8 cereal grains and 13 agro industrial by-products (AIBP). It was observed that lignin (% NDF) was lowest in white maize (5.28) and highest in gram khanda (30.93). NPN (%SP) content was highest in gram churi (73.32) and lowest in wheat grains (10.85). Among grains, values (%) for protein fraction PA, PB1, PB2, PB3 and fraction PC were found to be highest for oat (10.58), wheat (26.85), pearl millet (72.08),barley (17.63) and pearl millet (7.09), respectively while for AIBP, maximum values were observed for gram churi (32.67), beet pulp (56.98), gram khanda (82.61), paddy straw (43.39) and in gram husk (25.07), respectively. For AIBP, Carbohydrate fractions, CB1 content was highest (42.71) in wheat bran and CA was highest (61.74) in DDGS. It was observed that AIBP had more unavailable carbohydrate fraction (CC) in comparison to grains. Average values of IVDMD and IVOMD for grains were observed to be 89.78% and 90.80%, and for AIBP 75.19% and 75.23%, respectively. Average gas production (ml/200 mg) was found to be 57.85% for grains and 33.60% for AIBP. It was found that average methane generation (g/kg IVDMD) was comparable for grains and agro industrial by-products. Therefore, the study's findings may help to clarify more about the digestibility and gas production of agro industrial by products and cereal grains for more precise and environment friendly feed formulation for dairy animals.
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Aboagye I A, Christine L, Rosser V S, Baron and Karen A. B. 2021.In Vitro Assessment of Enteric Methane Emission Potential of Whole-Plant Barley, Oat, Triticale and Wheat.Animals (Basel).11(2): 450. doi: 10.3390/ani11020450
Ahmed E,Kholif O,Matloup, H, Morsy T, Abdo, MM, Abu Elella, AA, Uchenna Y,Anele, Kendall and Swanson C. 2017. Rosemary and lemongrass herbs as phytogenic feed additives to improve efficient feed utilization, manipulate rumen fermentation and elevate milk production of Damascus goats. Livestock Science204:39-46
AOAC. 1995. Official methods of analysis 16th Ed. Association of official analytical chemists. Washington DC, USA
AOAC. 1997. Association of Official Analytical Chemists International Official Methods of Analysis. 16th Edition, AOAC, Arlington.
Bose B K S, Kundu S S, Tho N T B, Sharma V K and Sontakke UB. 2014. Residual feed intake as a feed efficiency selectiontool and its relationship with feed intake, performance and nutrient utilization in Murrah buffalo calves. Tropical Animal Health and Production 46: 615–21
Das LK, Kundu SS, Kumar D and Datt C. 2014.The evaluation of metabolizable protein content of some indigenousfeedstuffs used in ruminant nutrition.Veterinary World 4:257-261.
Datt C, Sharma VK, Dudi K, Baban BN, Sharma Ph, Negesse T, Kundu SS, Dutta MM, Gupta R, and Singh D. 2017. Residual feed intake as a tool for selecting more efficient animals: A Review. Indian Journal of Animal Nutrition 34(3): 238-255.
Dixit S and Kundu SS. 2022.Carbohydrate and Protein Fractionations of commonly used forages and agro-industrial byproducts as per Cornell Net Carbohydrate and Protein system (CNCPS).Journal of Livestock Science 13(3):182-187. DOI:10.33259/JLivestSci.2022.182-187.
Eisler MC, LeeMRF,Tarlton JF,MartinGB,Beddington J,DungaitJAJ, Greathead H,LiuJ, Mathew S andMillerH. 2014.Steps to sustainable livestock.Nature507:32–34. doi:10.1038/507032a
Garg MR, Sherasia PL, Phondba BT, Shelke SK and Patel CT. 2013.Effect of Feeding Balanced Ration on Milk Production, Enteric Methane Emission and Metabolic Profile in Crossbred Cows under Field Conditions.Indian Journal of Dairy Science66(2): 113-119
Goering HK and Van Soest, PJ. 1970. Forage fiber analyses (apparatus, reagents, procedures, and some applications) (No. 379). US Agricultural Research Service.
Gupta A, Singh S, Kundu SS and Jha N. 2011.Evaluation of tropical feedstuffs for carbohydrate and protein fractions by CNCP system.Indian Journal of Animal Sciences81(11): 1154–1160.
Kaithwas M, Singh S, Prusty S, Mondal G and Kundu SS. 2020.Evaluation of legume and cereal fodders for carbohydrate and protein fractions, nutrient digestibility, energy and forage quality.Range Management and Agroforestry41(1): 126-132.
Kamble AB, Kundu SS, Shelke SK, Datt C and Jha N. 2010. Evaluation of concentrate feedstuffs for carbohydrate and protein fractions and in vitro methane production, Indian Journal of Animal Nutrition27: 109-115.
Kannan A, Bhar R, Raotra S and Mal G. 2017. Nutrient composition, in vitro true digestibility and methane production potential of feed resources of North Western Himalayan region. Indian Journal of Animal Sciences 87(10):1243-1250. DOI:10.56093/ijans.v87i10.75292.
Kour D, Sharma VK, Sharma RK, Pathak AK and Rastogi A. 2023. Evaluation of native medicinal plants as feed additives in the sheep ration. Indian Journal of Animal Science 93 (11): 1091–1097.
Lanzas C, Sniffen CJ, Seo S, Tedeschi LO and Fox DG. 2007. A revised CNCPS feed carbohydrate fractionation scheme for formulating rations for ruminant. Animal Feed Science and Technology136(3&4): 167-190.
Makkar HPS, Blümmel M and Becker K. 1995.Formation of complexes between polyvinyl pyrrolidones or polyethylene glycols and tannins, and their implication in gas production and true digestibility in in vitro techniques.British Journal of Nutrition73(6): 897-913
Menke KH and Steingass H. 1988. Estimation of the Energetic Feed Value Obtained from Chemical Analysis and in Vitro Gas Production Using Rumen Fluid. Animal Research and Development28: 7-55.
NIANP. 2013. Vision 2025. Icar-National Institute of Animal Nutrition and Physiology, Adugodi, Bengaluru 560030, India.
Patra AK. 2017. Prediction of enteric methane emission from cattle using linear and non-linear statistical models in tropical production systems. Mitigation and Adaptive Strategies for Global Change 22, 629–650. https://doi.org/10.1007/s11027-015-9691-7.
Prusty S, Kundu SS, Kumar V and Datt C. 2013.Dry Matter and Neutral Detergent Fiber Degradation Kinetics of Roughages in Relation to Carbohydrate and Protein Fractions.Indian Journal of Animal Nutrition 30(4): 374-380.
Reddy VS, Nagalakshmi D, VenkateswarluM and Rathod S. 2021.Rumen Degradability and In Vitro Fermentation Characteristicsof Various Cereal Grains.Indian Journal of Animal. Nutrition 38 (4): 392-399. doi: 10.5958/2231-6744.2021.00056.6.
Santoso B, Mwenya B, SarC and Takahashi J. 2007. Methane production and energy partition in sheep fed timothysilage- or hay-based diets. Indonesian Journal of Animal and Veterinary Science 12(1): 27-33.
SAS. 2002. Statistical Analysis System (SAS) Institute. SAS/STAT User’s Guide.Version 8, 6th Edition, SAS Institute, Cary, 112.
Sastry VRB, Kamra DN and Pathak NN. 1991. Laboratory Manual of Animal Nutrition. Centreof Advance Studies, Indian Veterinary Research Institute, Izatnagar, India, pp. 116-117.
Sharma N, Kundu SS and Tariq H. 2018. Chemical composition and CNCPS protein fractions of some feed ingredients used in livestock. International Journal of Chemical Studies6(2): 3722-3724.
Sharma Ph, Datt C, Baban BN, Kundu SS, Tyagi N, Sharma VK. 2016. Effect of Inclusion of Different Levels of Culled Potatoes in Replacement of Maize Grain in the Concentrate Mixture on Feed Intake, Nutrient Utilization and Growth in Sahiwal Calves. Indian Journal of Animal Nutrition 33(1):17-21.
Sharma VK, Tomar SK, Kundu SS, Jain P, Jha P, Kumar M and Singh Y. 2013. Effect of feeding different levels of sugarcane tops with concentrate mix/mustard cake on growth, nutrient intake and digestibility in buffalo calves. Indian Journal of Animal Nutrition 66(5): 407-411.
Sniffen CJ, O'ConnorJD,Van SoestPJ, Fox DG and Russell J B. 1992. A Net Carbohydrate and Protein System for evaluating cattle diets: II. Carbohydrate and protein availability.Journal of Animal Science70(11):3562-77. DOI:10.2527/1992.70113562x
Takahashi J. 2001. Nutritional manipulation of methanogenesis inruminants.Asian-Australasian Journal of Animal Science14 (special Issue):131-135
Theodorou MK, Williams BA, Dhanoa MS, McAllen AB and France J. 1994.A simple gas production method using a pressure transducer to determine the fermentation kinetics of ruminant feeds.Animal Feed Science and Technology48:185-197. https://doi.org/10.1016/0377-8401(94)90171-6
Van Soest PJ. 1994. Nutritional Ecology of Ruminants. 2nd Edition, Cornell University Press, Ithaca, London, 476.https://doi.org/10.7591/9781501732355.
Van Soest, PJ, Robertson JB and Lewis BA. 1991. Methods of Dietary Fiber, Neutral Detergent Fiber and Non-Starch Polysaccharides in Relation to Animal Nutrition. Journal of Dairy Science, 74, 3583-3597.http://dx.doi.org/10.3168/jds.S0022-0302(91)78551-2
Widiawati Y and Thalib A. 2007. Comparison fermentation kinetics (in vitro) of grass and shrub legume leaves: The pattern of VFA concentration, estimated CH4 and microbial biomass production. Journal of Veterinary and Animal Science12:96–104.
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