Characterization of membrane-associated non-genomic progesterone receptors in cattle bull spermatozoa

VIKAS  SACHAN; SOUMEN  CHOUDHURY; DILIP K  SWAIN; ANUJ  KUMAR; ATUL  SAXENA; PRATISHTHA  SHARMA; AVANEESH KUMAR  SINGH

doi:10.56093/ijans.v94i12.153327

Authors

VIKAS SACHAN COVAS, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut, Uttar Pradesh
SOUMEN CHOUDHURY COVSc & AH, UP Pandit Deen Dayal Upadhaya Pashuchikitsa Vigyan Vishwavidyalaya Evam Go-Anusandhan Sansthan, Mathura, Uttar Pradesh
DILIP K SWAIN COVSc & AH, UP Pandit Deen Dayal Upadhaya Pashuchikitsa Vigyan Vishwavidyalaya Evam Go-Anusandhan Sansthan, Mathura, Uttar Pradesh
ANUJ KUMAR COVSc & AH, UP Pandit Deen Dayal Upadhaya Pashuchikitsa Vigyan Vishwavidyalaya Evam Go-Anusandhan Sansthan, Mathura, Uttar Pradesh
ATUL SAXENA COVSc & AH, UP Pandit Deen Dayal Upadhaya Pashuchikitsa Vigyan Vishwavidyalaya Evam Go-Anusandhan Sansthan, Mathura, Uttar Pradesh
PRATISHTHA SHARMA Lighthouse Community Charter School, Hegenberger Rd, Oakland 94621, California, USA
AVANEESH KUMAR SINGH International Institute of Veterinary Education and Research, Rohtak, Haryana

https://doi.org/10.56093/ijans.v94i12.153327

Keywords:

Cattle, Immunolocalization, Non-genomic receptors, Progesterone, Spermatozoa

Abstract

The present study aimed to examine the molecular existence of the non-genomic progesterone receptors (P4) and their location in Hariana bull spermatozoa. The investigation involved the collection and processing of 64 semen ejaculates from four Hariana bulls. Following spermatozoa protein isolation, immunoblotting revealed the presence of 56 kDa protein corresponding to progesterone (P4) membrane receptors, and the results were validated using RT-PCR, which indicated an amplicon of 466 bp. Immuno-fluorescence technique was used to confirm the location of P4-membrane receptors in spermatozoa. The present study revealed the presence of 56 kDa protein representing progesterone receptors at the acrosome as well as post-acrosomal region of Hariana cattle bull spermatozoa.

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