A comprehensive serosurveillance of foot-and-mouth disease in pig population of Mizoram state in eastern Himalayan agro-climatic region in India

MANORANJAN  ROUT; ESTHER LALZOLIANI  RALTE; CHHAKCHHUAK  NEIHTHANGPUII; JAJATI KESHARI  MOHAPATRA; SARAVANAN  SUBRAMANIAM; RABINDRA PRASAD  SINGH

doi:10.56093/ijans.v95i12.154381

Authors

MANORANJAN ROUT ICAR-National Institute on Foot and Mouth Disease, Bhubaneswar – 752 050, India
ESTHER LALZOLIANI RALTE AICRP on Foot and Mouth Disease, Directorate of Animal Husbandry and Veterinary Services, Khatla – 796 001, Aizawl, Mizoram, India
CHHAKCHHUAK NEIHTHANGPUII AICRP on Foot and Mouth Disease, Directorate of Animal Husbandry and Veterinary Services, Khatla – 796 001, Aizawl, Mizoram, India
JAJATI KESHARI MOHAPATRA ICAR-National Institute on Foot and Mouth Disease, Bhubaneswar – 752 050, India
SARAVANAN SUBRAMANIAM ICAR-National Institute on Foot and Mouth Disease, Bhubaneswar – 752 050, India
RABINDRA PRASAD SINGH ICAR-National Institute on Foot and Mouth Disease, Bhubaneswar – 752 050, India

https://doi.org/10.56093/ijans.v95i12.154381

Keywords:

3AB3 NSP ELISA, Foot-and-mouth disease, India, LPB ELISA, Mizoram, Pig

Abstract

The primary objective of this study was to estimate the seroprevalence of foot-and-mouth disease virus (FMDV) 3AB3 nonstructural protein (NSP) antibodies in pig population of Mizoram. The 3AB3 NSP indirect ELISA, used for bovines, was modified using anti-porcine IgGhorse radish peroxidase (HRP) conjugate and successfully applied for serosurveillance of FMD in pig population of Mizoram. In total, 2996 randomly collected pig serum samples were tested using 3AB3 NSP ELISA, wherein 373 samples (12.44%) were found positive for NSP antibodies indicating past exposure to FMDV. Further, a set of representative serum samples was subjected to liquid phase blocking (LPB) ELISA to assess the level of protective structural antibody against FMDV serotypes O, A and Asia 1, but none of the pigs were found to have a protective log10 antibody titre of ≥1.8 against any of the three FMDV serotypes, indicating a ‘no vaccination’ situation in pigs in the region. FMDV-infected pigs being the amplifier hosts, excrete enormous quantity of virus and may pose a severe threat to other domestic livestock. Hence, pigs need to be included in ongoing surveillance and control measures, including prophylactic vaccination coupled with zoosanitary measures.

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