Genetic characterization and genotyping of β -lactamase producing Salmonella enterica serovars Enteritidis and Typhimurium isolated from livestock and poultry samples

C BINDU  KIRANMAYI; N  SUBHASHINI; T SRINIVASA  RAO; B  SURESH

doi:10.56093/ijans.v95i2.154836

Authors

C BINDU KIRANMAYI NTR College of Veterinary Science, Gannavaram, Krishna District – 521102, Andhra Pradesh, India
N SUBHASHINI NTR College of Veterinary Science, Gannavaram, Krishna District – 521102, Andhra Pradesh, India
T SRINIVASA RAO NTR College of Veterinary Science, Gannavaram, Krishna District – 521102, Andhra Pradesh, India
B SURESH NTR College of Veterinary Science, Gannavaram, Krishna District – 521102, Andhra Pradesh, India

https://doi.org/10.56093/ijans.v95i2.154836

Keywords:

AmpC, β-lactamases, Carbapenamases, Enteritidis, ESBL, Typhimurium

Abstract

The present study was designed to detect the presence of β-lactamase producing Salmonella enterica Serovars Enteritidis and Typhimurium from faecal (60 cattle rectal, 108 sheep rectal, 163 pig rectal and 186 poultry cloacal swabs) and meat (55 beef, 98 mutton, 120 pork and 126 chicken) samples of different livestock and poultry. The isolates were detected by cultural isolation and further confirmed by PCR. Further these isolates were subjected to phenotypic detection and genotypic confirmation of different β-lactamases by PST and PCR, respectively. Out of 916 samples analysed, 18 Enteritidis and 14 Typhimurium were isolated, out of which β-lactamase producing S. Enteritidis and S. Typhimurium were detected in 14 (10 poultry cloacal, 2 chicken, 1 pork and 1 pig rectal) and 12 (10 chicken, 1 pork and 1 mutton) samples, respectively. Of 14 β-lactamase positive S. Enteritidis, 7 showed presence of TEM, 3 OXA, 2 SHV, one each for CTXM-1 and CTXM-9. Out of 12 β-lactamase positive S. Typhimurium, 8 showed presence of TEM, 2 for CTXM-9 and one each for OXA and CTXM-2. Genotyping of these Salmonella isolates by ERIC & REP-PCR has differentiated all the isolates.

Downloads

Download data is not yet available.

References

Adak G K, Long S M and O’Brien S J. 2002. Trends in indigenous foodborne disease and deaths, England and Wales, 1992 to 2000. Gut 51(6):832–41.

Afshari A, Baratpour A, Khanzade S and Jamshidi, A. 2018. Salmonella Enteritidis and Salmonella Typhimurium identification in poultry carcasses. Iran Journal of Microbiology 10(1): 45-50.

Bindu Kiranmayi Ch, Subhashini N, Srinivasa Rao T, Suresh B, Venkata Chaitanya P and Bhavana B. 2021. Detection of β-lactamase-producing Proteus mirabilis strains of animal origin in Andhra Pradesh, India and their genetic diversity. Journal of Food Protection 84(8): 1374-1379. doi: 10.4315/ JFP-20-399.

Bush K and Jacoby G A. 2010. Updated functional classification of β-lactamases. Antimicrobial Agents Chemotherapy 54(3):969– 76.

Dallene CA, Da Costa D, Decre C, Favier and Arlet G. 2010. Development of a set of multiplex PCR assays for the detection of genes encoding important beta-lactamases in Enterobacteriaceae. The Journal of Antimicrobial Chemotherapy 65(3): 490–95. https://doi.org/10.1093/jac/ dkp498.

Diarra S, Delaquis P, Rempel H, Bach S, Harlton C, Aslam M, Pritchard J and Topp E. 2014. Antibiotic Resistance and Diversity of Salmonella enterica Serovars Associated with Broiler Chickens. Journal of Food Protection 77(1): 40-49. https://doi.org/10.4315/0362-028.JFP-13-251.

Drieux L, Brossier F, Sougakoff W and Jarlier V. 2008. Phenotypic detection of extended spectrum beta-lactamase production in Enterobacteriaceae: review and bench guide. Clinical Microbiology and Infections 14: 90-103.

EFSA, European Food Safety Authority. 2022. The European Union One Health 2022 Zoonoses Report. https://doi. org/10.2903/j.efsa.2023.8442.

Finstad S, O’Bryan C A, Marcy J A, Crandall P G and Ricke S C. 2012. Salmonella and broiler processing in the United States: relationship to foodborne salmonellosis. Food Research International 45: 789-94.

Hunter P R and Gaston M A. 1988. Numerical index of the discriminatory ability of typing systems: an application of Simpson’s index of diversity. Journal of Clinical Microbiology 26: 2465-6.

Jung HR, Lee Y J, Hong S, Yoon S, Lim S K and Lee Y J. 2023. Current status of β-lactam antibiotic use and characterization of β-lactam-resistant Escherichia coli from commercial farms by integrated broiler chicken operations in Korea. Poultry Science 102(12):1-7. https://doi.org/10.1016/j.psj.2023.103091.

Kirkwood M, Vohra P, Bawn M, Thilliez G, Pye H, Tanner J, Chintoan-Uta C, Branchu P, Petrovska L, Dallman T, Hall N, Stevens MP and Kingsley RA. 2021. Ecological niche adaptation of Salmonella Typhimurium U288 is associated with altered pathogenicity and reduced zoonotic potential. Communications Biology 4: 498. https://doi. org/10.1038/s42003-021-02013-4.

Manoharan A, Sugumar M, Kumar A, Jose H, Mathai D and ICMR-ESBL study group. 2012. Phenotypic and molecular characterization of AmpC β-lactamases among E. coli, Klebsiella spp. and Enterobacter spp. form five Indian medical centres. Indian Journal of Medical Research 135(3): 359.

Mehta A, Mehta YR and Rosato YB. 2002. ERIC- and REP-PCR amplify non-repetitive fragments from the genome of Drechslera avenae and Stemphylium solani. FEMS Microbiology Letters 211: 51-5. https://doi. org/10.1111/j.1574-6968.2002.tb11202.x.

Mohapatra BR, Broersma K and Mazumder A. 2007. Fecal Comparison of five rep‐PCR genomic fingerprinting methods for differentiation of Escherichia coli from humans, poultry and wild birds. FEMS Microbiology Letters 277(1): 98-106. doi: 10.1111/j.1574-6968.2007.00948.x.

Parry CM. 2003. Antimicrobial drug resistance in Salmonella enterica. Current Opinion in Infectious Diseases 16: 467-72.

Soumet C, Ermel G, Rose V, Rose N, Drouin P, Salvat G and Colin P. 1999. Identification by a multiplex PCR-based assay of Salmonella Typhimurium and Salmonella Enteritidis stains from environmental swabs of poultry houses. Letters in Applied Microbiology 29:1-6.

Suresh Y, Bindu Kiranmayi CH, Srinivasa Rao T, Srivani M, Subhashini N, Chaitanya G, Swathi Vimala B and Suresh B. 2019. Multidrug resistance and ESBL profile of Salmonella serovars isolated from poultry birds and foods of animal origin. The Pharma Innovation Journal 8(4): 277-82.

Syamily S, Ramesh KS and Revathi S. 2023. Salmonella infection in poultry: A review on the pathogen and control strategies. Microorganisms 11(11): 2814. https://doi.org/10.3390/ microorganisms11112814.

USDA. 1996. Tracking food borne pathogens from farm to table: data needs to evaluate control points. Conference proceedings. 9-10.

Van Denn Bogaard AE and Stobberingh E.E. 1999. Antibiotic usage in animals: impact on bacterial resistance and public health. Drugs 58: 589-607.

Wang X, Jordan IK and Mayer LW. 2015. A Phylogenetic Perspective on molecular epidemiology (Ch. 29). Molecular Medical Microbiology (Second Edition). Vol I: 517-536. https://doi.org/10.1016/B978-0-12-397169-2.00029-9.