Effect of nickel supplementation on the antioxidantand immune status of growing lambs

A  GANGWAR; DS  SAHU; G  CHANDRA; SP  YADAV; DEBASHIS  ROY; RK  SINGH; AHMAD  FAHIM; DK  YADAV; PS  MAURYA; PK  MAURYA

doi:10.56093/ijans.v95i12.155135

Authors

A GANGWAR Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut Uttar Pradesh 250 110 India
DS SAHU Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut Uttar Pradesh 250 110 India
G CHANDRA Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut Uttar Pradesh 250 110 India
SP YADAV Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut Uttar Pradesh 250 110 India
DEBASHIS ROY Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut Uttar Pradesh 250 110 India
RK SINGH Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut Uttar Pradesh 250 110 India
AHMAD FAHIM Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut Uttar Pradesh 250 110 India
DK YADAV Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut Uttar Pradesh 250 110 India
PS MAURYA Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut Uttar Pradesh 250 110 India
PK MAURYA Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut Uttar Pradesh 250 110 India

https://doi.org/10.56093/ijans.v95i12.155135

Keywords:

Antioxidant status, Gmmune status, Growing lambs, Nickel

Abstract

The present investigation aimed to explore the impact of nickel (Ni) supplementation on the antioxidant and immune status of growing Muzaffarnagari lambs. For the study, 18 healthy Muzaffarnagari lambs (30.45±3.67 kg) body weight and age (10.33±0.67 months) were randomly divided into 3 groups of 6 each. The composition of basal diet was similar across all three groups, except for Ni supplementation at dose level of 0.0 (control), 1.5 (Ni1.5), and 3.0 (Ni3.0) mg/kg DM, administered to the respective groups over a 90-day study period. Blood samples were collected by jugular puncture on days 0, 15, 30, 45, 60, 75, and 90 of the study in the morning, before feeding and watering. The superoxide dismutase (SOD), total antioxidant activity (TAA), and thiobarbituric acid reactive substance (TBARS) did not vary statistically among the groups, control, Ni1.5, and Ni3.0, whereas, catalase activity declined significantly (P<0.05) in groups that received 1.5 or 3.0 mg Ni/kg DM as compared to the control group. Nickel supplementation did not influence the concentration of haemoglobin (Hb), total leucocyte count (TLC), and neutrophils. Lymphocyte concentration was statistically (P<0.05) higher in lambs who received Ni at a dose of 3.0 mg/kg DM as compared to other animals. Plasma levels of total immunoglobulin (TIG) were higher in treatment groups of lambs fed either 1.5 or 3.0 mg Ni/kg DM than in the control group. The findings of the present study indicated that nickel supplementation at both doses (1.5 and 3.0 mg/kg DM) improved immune function; however, it did not significantly affect the antioxidant status of growing lambs.

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