Effect of glucosamine supplementation on performance and egg quality of laying chicken
362 / 259
Keywords:
Age at first egg, Egg quality, Estrogen, Glucosamine hydrochloride, LeptinAbstract
The experiment was conducted to explore the effect of supplemental glucosamine on egg production and quality performance of Vanaraja Chickens. The chickens of same age (8 week) were divided into three equal groups i.e. Control (C), Group 10, and Group 20. Birds in group G10 and G20 were supplemented with glucosamine hydrochloride @ doses of 10 mg/kg and 20 mg/kg of body weight/day respectively, via drinking water individually in a plastic container kept in cages. The amount of the supplemented glucosamine was based on their body weight taken fortnightly. The effect of glucosamine on laying performance of the treated groups was compared with control group. For hormonal estimation, blood samples were collected at 15 weeks and 22 weeks of age. Data were analysed using ANOVA (Analysis of Variance) and paired t-test. It was found that the age at first egg laying (AFE) was lesser in G10 and G20 as compared to control group. Egg production was highest in the G10 group based on initial six month records. There was no significant difference of glucosamine supplementation in the important quality parameters including egg weight, shell weight, shell thickness, yolk weight and albumin weight. There was significant increase in the plasma estrogen and leptin level between 15 weeks and 22 weeks of age in all the three groups indicating their role in attaining puberty. Our results indicate that glucosamine hydrochloride may have role in egg laying performance in poultry.
Downloads
References
Ahima R S, Dushay J, Flier S N, Prabakaran D and Flier J S. 1997. Leptin accelerates the onset of puberty in normal female mice. The Journal of Clinical Investigation 99(3): 391–95.
Considine R V, Cooksey R C, Williams L B, Fawcett R L, Zhang P, Ambrosius W T, Whitfield R M, Jones R, Inman M, Huse J and McClain D A. 2000. Hexosamines regulate leptin production in human subcutaneous adipocytes. The Journal of Clinical Endocrinology and Metabolism 85(10): 3551–56.
Feng C, Yuan T, Wang S, Liu T, Tao S, Han D and Wang J. 2020. Glucosamine supplementation in premating drinking water improves within-litter birth weight uniformity of rats partly through modulating hormone metabolism and genes involved in implantation. BioMed Research International 2020(1): 1630890.
Garcia M R, Amstalden M, Williams S W, Stanko R L, Morrison C D, Keisler, D H, Nizielski S E and Williams G L. 2002. Serum leptin and its adipose gene expression during pubertal development, the estrous cycle, and different seasons in cattle. Journal of Animal Science 80(8): 2158–67.
Gündüz B Ü. 2023. Reproductive development of Japanese Quail (Coturnix coturnix japonica) in males and females subjected to leptin injections. Acta Veterinaria Eurasia 49(3): 149–54.
Ha Y W, Son M J, Yun K S and Kim Y S. 2007. Relationship between eggshell strength and keratan sulfate of eggshell membranes. Comparative Biochemistry and Physiology Part A: Molecular and Integrative Physiology 147(4): 1109–15.
Hanlon C, Takeshima K and Bédécarrats G Y. 2022. Changes in the control of the hypothalamic-pituitary gonadal axis across three differentially selected strains of laying hens (Gallus gallus domesticus). Frontiers in Physiology 12: 651491.
Kamal M, Youssef, I M, Khalil H A, Ayoub M A. and Hashem, N M. 2022. Multifunctional role of chitosan in farm animals: a comprehensive review. Annals of Animal Science 23(1): 69–86.
Li X, Ye H, Su T, Hu C, Huang Y, Fu X, Zhong Z, Du X and Zheng Y. 2023. Immunity and reproduction protective effects of Chitosan Oligosaccharides in Cyclophosphamide/Busulfan-induced premature ovarian failure model mice. Frontiers in Immunology 14: 1185921.
Li Y, Zhang Q, Feng Y, Yan S, Shi B, Guo X, Zhao Y and Guo Y. 2022. Dietary chitosan supplementation improved egg production and antioxidative function in laying breeders. Animals 12(10): 1225.
Liu X T, Lin X, Mi Y L, Zeng W D and Zhang C Q. 2018. Age-related changes of yolk precursor formation in the liver of laying hens. Journal of Zhejiang University Science B 19(5): 390.
Liu Z, Sun X, Cai C, He W, Zhang F and Linhardt R J. 2016. Characteristics of glycosaminoglycans in chicken eggshells and the influence of disaccharide composition on eggshell properties. Poultry Science 95(12): 2879–88
Ma´cˇajova´ M, Lamosˇova´ D and Zeman M. 2002. Role of leptin in Japanese quail development. Acta Veterinaria Brno 71(4): 473–79.
Martins J M D S, Santos Neto L D D, Gomides L P S, Fernandes E D S, Sgavioli S, Stringhini J H, Leandro N S M and Café M B. 2020. Performance, nutrient digestibility, and intestinal histomorphometry of broilers fed diet supplemented with chondroitin and glucosamine sulfates. Revista Brasileira de Zootecnia 49.
Mehlhorn J, Höhne A, Baulain U, Schrader L, Weigend S and Petow S. 2022. Estradiol-17ß is Influenced by age, housing system, and laying performance in genetically divergent laying hens (Gallus gallus). Frontiers in Physiology 13: 954399.
Muñoz-Gutiérrez M, Blache D, Martin G B and Scaramuzzi R J. 2002. Folliculogenesis and ovarian expression of mRNA encoding aromatase in anoestrous sheep after 5 days of glucose or glucosamine infusion or supplementary lupin feeding. Reproduction-Cambridge 124(5): 721–731.
Ni Y, Lv J, Wang S and Zhao R. 2013. Sexual maturation in hens is not associated with increases in serum leptin and the expression of leptin receptor mRNA in hypothalamus. Journal of Animal Science and Biotechnology 4(1): 1–7.
Paczoska-Eliasiewicz H E, Proszkowiec-Weglarz M, Proudman J, Jacek T, Mika M, Sechman A, Rzasa J and Gertler A. 2006. Exogenous leptin advances puberty in domestic hen. Domestic Animal Endocrinology 31(3): 211–26.
Panigrahy K K, Kumaresh B, Gupta S K and Sasmita, P. 2016. Effect of serum leptin concentration on cognitive ability of male and female Vanaraja chickens. Asian Journal of Biological Sciences 11(2): 309–312.
Pizzolatti A LA, Gaudig F, Seitz D, Roesler C R and Salmoria, G V. 2018. Glucosamine Hydrochloride and N-Acetylglucosamine Influence the Response of Bovine Chondrocytes to TGF-β3 and IGF in Monolayer and Three-Dimensional Tissue Culture. Tissue Engineering and Regenerative Medicine 15(6): 781–791
Ryczko M C, Pawling J, Chen R, Anas M, Rahman A, Yau K, Copeland J K, Zhang C, Surendra A, Guttman D S, Figeys D and Dennis J W. 2016. Metabolic Reprogramming by Hexosamine Biosynthetic and Golgi N-Glycan Branching Pathways. Scientific Reports 6(1): 23043.
Tan Y G, Xu L, Cao Y, Zhou W and Yin Z. 2021. Effect of age at first egg on reproduction performance and characterization of the hypothalamo–pituitary–gonadal axis in chickens. Poultry Science 100(9): 101325.
Van der Klein S A S, Hadinia S H, Robinson F E, Bédécarrats G Y and Zuidhof M J. 2019. A model of pre-pubertal broiler breeder estradiol-17β levels predicts advanced sexual maturation for birds with high body weight or short juvenile day-length exposure. Poultry Science 98(10): 5137–45.
Wang J, Liu R, Hawkins M, Barzilai M and, Rossetti L. 1998. A nutrient-sensing pathway regulates leptin gene expression in muscle and fat. Nature 393(6686): 684-88.
Wang Y, Huang Y, Zhou P, Lu S, Lin J, Wen G, Shi X and Guo Y. 2024. Effects of dietary glucosamine sulfate sodium on early laying performance and eggshell quality of laying hens. Poultry Science 103(9): 82.
Xu Q, Azzam M M M, Zou X and Dong X. 2020. Effects of chitooligosaccharide supplementation on laying performance, egg quality, blood biochemistry, antioxidant capacity and immunity of laying hens during the late laying period. Italian Journal of Animal Science 19(1): 1180–87.
Zhu H J, Li S J, Pan H, Li N, Zhang D, Wang L J, Yang H B, Wu Q and Gong F Y. 2016. The changes of serum leptin and kisspeptin levels in chinese children and adolescents in different pubertal stages. The Journal of Endocrinology 2016(1): 6790794.
Downloads
Submitted
Published
Issue
Section
License
Copyright (c) 2025 The Indian Journal of Animal Sciences

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
The copyright of the articles published in The Indian Journal of Animal Sciences is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.