A cross-sectional study to understand the correlation among Salmonella enterica infection and Lactobacillus reuteri concentration in fecal samples of post weaning piglets and their association in causation of diarrhea

NOVIK YANA  VIKTOROVNA; SITA PRASAD  TIWARI; BALESHWARI  DIXIT; MIRONOVA TATYANA  EVGENIEVNA; CHEREPUSHKINA VICTORIA  SERGEEVNA; AFONYUSHKINVASILY  NIKOLAEVICH; KORNEVA MARINA  VYACHESLAVOVNA; DAVYDOVA NATALIA  VICTOROVNA; YURCHENKO ELIZAVETA  EVGENIEVNA

doi:10.56093/ijans.v95i4.155217

Authors

NOVIK YANA VIKTOROVNA Federal State Educational Institution of Higher Education, Novosibirsk State Agrarian University, Novosibirsk, Russia
SITA PRASAD TIWARI Nanaji Deshmukh Veterinary Science University, Jabalpur, 482001, M.P., India
BALESHWARI DIXIT Siberian Federal Scientific Center of Agrobiotechnologies RAS, Novosibirsk Region, Novosibirsk District, S. Krasnoobsk, Russia
MIRONOVA TATYANA EVGENIEVNA Federal State Educational Institution of Higher Education, Novosibirsk State Agrarian University, Novosibirsk, Russia
CHEREPUSHKINA VICTORIA SERGEEVNA Siberian Federal Scientific Center of Agrobiotechnologies RAS, Novosibirsk Region, Novosibirsk District, S. Krasnoobsk, Russia
AFONYUSHKINVASILY NIKOLAEVICH Siberian Federal Scientific Center of Agrobiotechnologies RAS, Novosibirsk Region, Novosibirsk District, S. Krasnoobsk, Russia
KORNEVA MARINA VYACHESLAVOVNA Federal State Educational Institution of Higher Education, Novosibirsk State Agrarian University, Novosibirsk, Russia
DAVYDOVA NATALIA VICTOROVNA Siberian Federal Scientific Center of Agrobiotechnologies RAS, Novosibirsk Region, Novosibirsk District, S. Krasnoobsk, Russia
YURCHENKO ELIZAVETA EVGENIEVNA Federal State Educational Institution of Higher Education, Novosibirsk State Agrarian University, Novosibirsk, Russia

https://doi.org/10.56093/ijans.v95i4.155217

Keywords:

Diarrheal syndrome, Lactobacilli reuteri, Post-weaning, Piglets, Salmonella

Abstract

Interaction among host gut microbiome and disease-causing organisms play an important role in the establishment of infection. Considering it, the relationship between the concentration of Lactobacillus reuteri and the level of Salmonella infection was assessed in the feces of post weaning piglets, in reference to diarrheic (sick) and healthy piglets. Eighty fecal samples from piglets (40-50 days of age) of eight different pig farms were examined. Piglets were classified as sick (diarrhea) and healthy (norm) by Indikal indicator paper method. Fecal samples were processed for the extraction of genomic DNA of Lactobacilli and Salmonella and subjected to real-time PCR to detect concentration of microorganisms in the sample. The results indicated that Salmonella infection did not directly affect the development of post weaning diarrheal syndrome in piglets. Positive correlation between the presence of reuteri and the PCR prevalence of salmonellosis in piglets was puzzling, since this contradicts a lot of literature data. The relationship between opportunistic intestinal microbiota and Lactobacilli of normal microflora is more complex under conditions of long-term coevolution. The probiotics based on L. reuteri may be more effective, provided that there was no long-term adaptation of Salmonella to Lactobacilli of this species.

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