Goat specific synthesized antimicrobial peptide β-defensin improves post-thaw sperm fertility

MANISH  KUMAR; RAVI  RANJAN; ALOK  BHARADWAJ; K  GURURAJ

doi:10.56093/ijans.v95i4.155592

Authors

MANISH KUMAR GLA University, Mathura, Uttar Pradesh, India
RAVI RANJAN ICAR-Central Institute for Research on Goats, Makhdoom, Farah, Mathura, Uttar Pradesh-281122, India
ALOK BHARADWAJ GLA University, Mathura, Uttar Pradesh, India
K GURURAJ ICAR-Central Institute for Research on Goats, Makhdoom, Farah, Mathura, Uttar Pradesh-281122, India

https://doi.org/10.56093/ijans.v95i4.155592

Keywords:

Antioxidant, Bacterial load, β-defensin, Cryopreservation

Abstract

Cryoprotectant selection and their formulation for semen extenders are critical factors that significantly influence sperm survivability and fertility after cryopreservation. Antimicrobial peptides offer a promising alternative to antibiotics in semen cryopreservation. The objective of this study was to improve the post thaw sperm survivability and fertility by supplementation of synthesized β-defensin antimicrobial peptide (βdef-AMP). βdef-AMP was designed and synthesized from outsource supplemented in ejaculates (control, 10 µM, 15 µM and 20 µM). βdef-AMP exhibited significant efficacy in reducing the bacterial load in all tested concentrations (10, 15, and 20 µM). The post- thaw sperm motility, live sperm, sperm membrane and acrosome integrity were significantly higher (p<0.05) in 15 µM fortified diluent. Malondialdehyde production was significantly lower (p<0.05) in 15 µM fortified diluent showing low lipid peroxidation. So, the supplementation of 15 µM βdef-AMP is advantageous for the cryopreservation of Barbari buck semen to improve sperm fertility and to reduce microbial load in semen.

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